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The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein

Nod-Like Receptor Pyrin domain-containing protein 6 (NLRP6), a member of the Nucleotide-oligomerization domain-Like Receptor (NLR) family of proteins, assembles together with the ASC protein to form an inflammasome upon stimulation by bacterial lipoteichoic acid and double-stranded DNA. Besides its...

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Autores principales: Mound, Abdallah, Goormachtigh, Gautier, Bray, Fabrice, Flament, Stéphanie, Rolando, Christian, Ruez, Richard, Martin, Nathalie, Decourcelle, Amélie, Dehennaut, Vanessa, Saliou, Jean-Michel, Chamaillard, Mathias, Abbadie, Corinne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858803/
https://www.ncbi.nlm.nih.gov/pubmed/36662875
http://dx.doi.org/10.1371/journal.pone.0279028
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author Mound, Abdallah
Goormachtigh, Gautier
Bray, Fabrice
Flament, Stéphanie
Rolando, Christian
Ruez, Richard
Martin, Nathalie
Decourcelle, Amélie
Dehennaut, Vanessa
Saliou, Jean-Michel
Chamaillard, Mathias
Abbadie, Corinne
author_facet Mound, Abdallah
Goormachtigh, Gautier
Bray, Fabrice
Flament, Stéphanie
Rolando, Christian
Ruez, Richard
Martin, Nathalie
Decourcelle, Amélie
Dehennaut, Vanessa
Saliou, Jean-Michel
Chamaillard, Mathias
Abbadie, Corinne
author_sort Mound, Abdallah
collection PubMed
description Nod-Like Receptor Pyrin domain-containing protein 6 (NLRP6), a member of the Nucleotide-oligomerization domain-Like Receptor (NLR) family of proteins, assembles together with the ASC protein to form an inflammasome upon stimulation by bacterial lipoteichoic acid and double-stranded DNA. Besides its expression in myeloid cells, NLRP6 is also expressed in intestinal epithelial cells where it may contribute to the maintenance of gut homeostasis and negatively controls colorectal tumorigenesis. Here, we report that NLRP6 is very faintly expressed in several colon cancer cell lines, detected only in cytoplasmic small dots were it colocalizes with ASC. Consequently, it is very hardly detected by standard western-blotting techniques by several presently available commercial antibodies which, in contrast, highly cross-react with a protein of 90kDa that we demonstrate to be unrelated to NLRP6. We report here these results to caution the community not to confuse the 90kDa protein with the endogenous human NLRP6.
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spelling pubmed-98588032023-01-21 The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein Mound, Abdallah Goormachtigh, Gautier Bray, Fabrice Flament, Stéphanie Rolando, Christian Ruez, Richard Martin, Nathalie Decourcelle, Amélie Dehennaut, Vanessa Saliou, Jean-Michel Chamaillard, Mathias Abbadie, Corinne PLoS One Research Article Nod-Like Receptor Pyrin domain-containing protein 6 (NLRP6), a member of the Nucleotide-oligomerization domain-Like Receptor (NLR) family of proteins, assembles together with the ASC protein to form an inflammasome upon stimulation by bacterial lipoteichoic acid and double-stranded DNA. Besides its expression in myeloid cells, NLRP6 is also expressed in intestinal epithelial cells where it may contribute to the maintenance of gut homeostasis and negatively controls colorectal tumorigenesis. Here, we report that NLRP6 is very faintly expressed in several colon cancer cell lines, detected only in cytoplasmic small dots were it colocalizes with ASC. Consequently, it is very hardly detected by standard western-blotting techniques by several presently available commercial antibodies which, in contrast, highly cross-react with a protein of 90kDa that we demonstrate to be unrelated to NLRP6. We report here these results to caution the community not to confuse the 90kDa protein with the endogenous human NLRP6. Public Library of Science 2023-01-20 /pmc/articles/PMC9858803/ /pubmed/36662875 http://dx.doi.org/10.1371/journal.pone.0279028 Text en © 2023 Mound et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mound, Abdallah
Goormachtigh, Gautier
Bray, Fabrice
Flament, Stéphanie
Rolando, Christian
Ruez, Richard
Martin, Nathalie
Decourcelle, Amélie
Dehennaut, Vanessa
Saliou, Jean-Michel
Chamaillard, Mathias
Abbadie, Corinne
The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
title The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
title_full The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
title_fullStr The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
title_full_unstemmed The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
title_short The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
title_sort nlrp6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858803/
https://www.ncbi.nlm.nih.gov/pubmed/36662875
http://dx.doi.org/10.1371/journal.pone.0279028
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