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Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor
The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additiona...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research Network of Computational and Structural Biotechnology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860152/ https://www.ncbi.nlm.nih.gov/pubmed/36698979 http://dx.doi.org/10.1016/j.csbj.2022.12.026 |
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author | Veverka, Pavel Brom, Tomáš Janovič, Tomáš Stojaspal, Martin Pinkas, Matyáš Nováček, Jiří Hofr, Ctirad |
author_facet | Veverka, Pavel Brom, Tomáš Janovič, Tomáš Stojaspal, Martin Pinkas, Matyáš Nováček, Jiří Hofr, Ctirad |
author_sort | Veverka, Pavel |
collection | PubMed |
description | The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases. |
format | Online Article Text |
id | pubmed-9860152 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Research Network of Computational and Structural Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-98601522023-01-24 Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor Veverka, Pavel Brom, Tomáš Janovič, Tomáš Stojaspal, Martin Pinkas, Matyáš Nováček, Jiří Hofr, Ctirad Comput Struct Biotechnol J Research Article The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases. Research Network of Computational and Structural Biotechnology 2022-12-19 /pmc/articles/PMC9860152/ /pubmed/36698979 http://dx.doi.org/10.1016/j.csbj.2022.12.026 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Veverka, Pavel Brom, Tomáš Janovič, Tomáš Stojaspal, Martin Pinkas, Matyáš Nováček, Jiří Hofr, Ctirad Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor |
title | Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor |
title_full | Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor |
title_fullStr | Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor |
title_full_unstemmed | Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor |
title_short | Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor |
title_sort | electron microscopy reveals toroidal shape of master neuronal cell differentiator rest – re1-silencing transcription factor |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860152/ https://www.ncbi.nlm.nih.gov/pubmed/36698979 http://dx.doi.org/10.1016/j.csbj.2022.12.026 |
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