Production and characterization of cell-penetrating recombinant botulinum neurotoxin type A

We recently reported that fusion of cell-penetrating peptides (CPPs) to botulinum neurotoxin type A (BoNTA) proteins could improve the efficiency of cellular uptake. Here, we describe steps to produce and evaluate CPP-BoNTA fusion proteins. We present procedures for the expression and purification o...

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Detalles Bibliográficos
Autores principales: Wei, Xuan, Hu, Jiage, Jiang, Liling, Li, Lu, Liu, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860155/
https://www.ncbi.nlm.nih.gov/pubmed/36853731
http://dx.doi.org/10.1016/j.xpro.2022.102026

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860155/
https://www.ncbi.nlm.nih.gov/pubmed/36853731
http://dx.doi.org/10.1016/j.xpro.2022.102026

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