Viral competition assay to assess the role of HIV-1 proteins in immune evasion

CD8+ T lymphocytes can recognize and eliminate cells infected by viruses. However, the human immunodeficiency virus (HIV-1) has developed mechanisms to evade CD8+ T-cell-mediated clearance. Here, we describe a protocol to assess the role of the HIV-1 protein Nef in immune evasion. The viral competit...

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Detalles Bibliográficos
Autores principales: Duette, Gabriel, Cronin, Samantha, Kelleher, Anthony D., Palmer, Sarah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860156/
https://www.ncbi.nlm.nih.gov/pubmed/36853860
http://dx.doi.org/10.1016/j.xpro.2022.102025
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author Duette, Gabriel
Cronin, Samantha
Kelleher, Anthony D.
Palmer, Sarah
author_facet Duette, Gabriel
Cronin, Samantha
Kelleher, Anthony D.
Palmer, Sarah
author_sort Duette, Gabriel
collection PubMed
description CD8+ T lymphocytes can recognize and eliminate cells infected by viruses. However, the human immunodeficiency virus (HIV-1) has developed mechanisms to evade CD8+ T-cell-mediated clearance. Here, we describe a protocol to assess the role of the HIV-1 protein Nef in immune evasion. The viral competition assay reveals the preferential killing of HIV-1-infected cells unable to express Nef. This methodology can be extended to study HIV-1 proteins involved in immune evasion and viral variants encoding cytotoxic T lymphocyte escape mutations. For complete details on the use and execution of this protocol, please refer to Duette et al. (2022).(1)
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spelling pubmed-98601562023-01-22 Viral competition assay to assess the role of HIV-1 proteins in immune evasion Duette, Gabriel Cronin, Samantha Kelleher, Anthony D. Palmer, Sarah STAR Protoc Protocol CD8+ T lymphocytes can recognize and eliminate cells infected by viruses. However, the human immunodeficiency virus (HIV-1) has developed mechanisms to evade CD8+ T-cell-mediated clearance. Here, we describe a protocol to assess the role of the HIV-1 protein Nef in immune evasion. The viral competition assay reveals the preferential killing of HIV-1-infected cells unable to express Nef. This methodology can be extended to study HIV-1 proteins involved in immune evasion and viral variants encoding cytotoxic T lymphocyte escape mutations. For complete details on the use and execution of this protocol, please refer to Duette et al. (2022).(1) Elsevier 2023-01-14 /pmc/articles/PMC9860156/ /pubmed/36853860 http://dx.doi.org/10.1016/j.xpro.2022.102025 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Duette, Gabriel
Cronin, Samantha
Kelleher, Anthony D.
Palmer, Sarah
Viral competition assay to assess the role of HIV-1 proteins in immune evasion
title Viral competition assay to assess the role of HIV-1 proteins in immune evasion
title_full Viral competition assay to assess the role of HIV-1 proteins in immune evasion
title_fullStr Viral competition assay to assess the role of HIV-1 proteins in immune evasion
title_full_unstemmed Viral competition assay to assess the role of HIV-1 proteins in immune evasion
title_short Viral competition assay to assess the role of HIV-1 proteins in immune evasion
title_sort viral competition assay to assess the role of hiv-1 proteins in immune evasion
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860156/
https://www.ncbi.nlm.nih.gov/pubmed/36853860
http://dx.doi.org/10.1016/j.xpro.2022.102025
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