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An autoactive NB-LRR gene causes Rht13 dwarfism in wheat
Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nu...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860330/ https://www.ncbi.nlm.nih.gov/pubmed/36417432 http://dx.doi.org/10.1073/pnas.2209875119 |
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author | Borrill, Philippa Mago, Rohit Xu, Tianyuan Ford, Brett Williams, Simon J. Derkx, Adinda Bovill, William D. Hyles, Jessica Bhatt, Dhara Xia, Xiaodi MacMillan, Colleen White, Rosemary Buss, Wolfram Molnár, István Walkowiak, Sean Olsen, Odd-Arne Doležel, Jaroslav Pozniak, Curtis J. Spielmeyer, Wolfgang |
author_facet | Borrill, Philippa Mago, Rohit Xu, Tianyuan Ford, Brett Williams, Simon J. Derkx, Adinda Bovill, William D. Hyles, Jessica Bhatt, Dhara Xia, Xiaodi MacMillan, Colleen White, Rosemary Buss, Wolfram Molnár, István Walkowiak, Sean Olsen, Odd-Arne Doležel, Jaroslav Pozniak, Curtis J. Spielmeyer, Wolfgang |
author_sort | Borrill, Philippa |
collection | PubMed |
description | Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker. |
format | Online Article Text |
id | pubmed-9860330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-98603302023-02-01 An autoactive NB-LRR gene causes Rht13 dwarfism in wheat Borrill, Philippa Mago, Rohit Xu, Tianyuan Ford, Brett Williams, Simon J. Derkx, Adinda Bovill, William D. Hyles, Jessica Bhatt, Dhara Xia, Xiaodi MacMillan, Colleen White, Rosemary Buss, Wolfram Molnár, István Walkowiak, Sean Olsen, Odd-Arne Doležel, Jaroslav Pozniak, Curtis J. Spielmeyer, Wolfgang Proc Natl Acad Sci U S A Biological Sciences Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker. National Academy of Sciences 2022-11-23 2022-11-29 /pmc/articles/PMC9860330/ /pubmed/36417432 http://dx.doi.org/10.1073/pnas.2209875119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Biological Sciences Borrill, Philippa Mago, Rohit Xu, Tianyuan Ford, Brett Williams, Simon J. Derkx, Adinda Bovill, William D. Hyles, Jessica Bhatt, Dhara Xia, Xiaodi MacMillan, Colleen White, Rosemary Buss, Wolfram Molnár, István Walkowiak, Sean Olsen, Odd-Arne Doležel, Jaroslav Pozniak, Curtis J. Spielmeyer, Wolfgang An autoactive NB-LRR gene causes Rht13 dwarfism in wheat |
title | An autoactive NB-LRR gene causes Rht13 dwarfism in wheat |
title_full | An autoactive NB-LRR gene causes Rht13 dwarfism in wheat |
title_fullStr | An autoactive NB-LRR gene causes Rht13 dwarfism in wheat |
title_full_unstemmed | An autoactive NB-LRR gene causes Rht13 dwarfism in wheat |
title_short | An autoactive NB-LRR gene causes Rht13 dwarfism in wheat |
title_sort | autoactive nb-lrr gene causes rht13 dwarfism in wheat |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860330/ https://www.ncbi.nlm.nih.gov/pubmed/36417432 http://dx.doi.org/10.1073/pnas.2209875119 |
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