Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish

Here, we provide an optimized protocol to observe the interactions between infiltrating immune cells and islet β cells using live imaging. This protocol is useful for the characterization of cell-cell interactions and for the direct visualization of immune cell migration to the principal pancreatic...

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Detalles Bibliográficos
Autores principales: Yang, Bingyuan, Zhang, Yue, Yang, Liu, Chen, Wenbiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860484/
https://www.ncbi.nlm.nih.gov/pubmed/36853669
http://dx.doi.org/10.1016/j.xpro.2022.102039
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author Yang, Bingyuan
Zhang, Yue
Yang, Liu
Chen, Wenbiao
author_facet Yang, Bingyuan
Zhang, Yue
Yang, Liu
Chen, Wenbiao
author_sort Yang, Bingyuan
collection PubMed
description Here, we provide an optimized protocol to observe the interactions between infiltrating immune cells and islet β cells using live imaging. This protocol is useful for the characterization of cell-cell interactions and for the direct visualization of immune cell migration to the principal pancreatic islet during islet inflammation. We describe the preparation of zebrafish transgenic lines and detail steps for setting up the fish for live confocal imaging. For more details on the use and execution of this protocol, please refer to Yang et al. (2022).(1)
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spelling pubmed-98604842023-01-22 Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish Yang, Bingyuan Zhang, Yue Yang, Liu Chen, Wenbiao STAR Protoc Protocol Here, we provide an optimized protocol to observe the interactions between infiltrating immune cells and islet β cells using live imaging. This protocol is useful for the characterization of cell-cell interactions and for the direct visualization of immune cell migration to the principal pancreatic islet during islet inflammation. We describe the preparation of zebrafish transgenic lines and detail steps for setting up the fish for live confocal imaging. For more details on the use and execution of this protocol, please refer to Yang et al. (2022).(1) Elsevier 2023-01-17 /pmc/articles/PMC9860484/ /pubmed/36853669 http://dx.doi.org/10.1016/j.xpro.2022.102039 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yang, Bingyuan
Zhang, Yue
Yang, Liu
Chen, Wenbiao
Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish
title Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish
title_full Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish
title_fullStr Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish
title_full_unstemmed Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish
title_short Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish
title_sort optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860484/
https://www.ncbi.nlm.nih.gov/pubmed/36853669
http://dx.doi.org/10.1016/j.xpro.2022.102039
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