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Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches
The study of adipogenesis is essential for understanding and treating obesity, a multifactorial problem related to body fat accumulation that leads to several life-threatening diseases, becoming one of the most critical public health problems worldwide. In this review, we propose to provide the high...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860640/ https://www.ncbi.nlm.nih.gov/pubmed/36676057 http://dx.doi.org/10.3390/life13010108 |
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author | Pamplona, Juliana Helena Zoehler, Bernardo Shigunov, Patrícia Barisón, María Julia Severo, Vanessa Rossini Erich, Natalie Mayara Ribeiro, Annanda Lyra Horinouchi, Cintia Delai da Silva Suzukawa, Andréia Akemi Robert, Anny Waloski Abud, Ana Paula Ressetti de Aguiar, Alessandra Melo |
author_facet | Pamplona, Juliana Helena Zoehler, Bernardo Shigunov, Patrícia Barisón, María Julia Severo, Vanessa Rossini Erich, Natalie Mayara Ribeiro, Annanda Lyra Horinouchi, Cintia Delai da Silva Suzukawa, Andréia Akemi Robert, Anny Waloski Abud, Ana Paula Ressetti de Aguiar, Alessandra Melo |
author_sort | Pamplona, Juliana Helena |
collection | PubMed |
description | The study of adipogenesis is essential for understanding and treating obesity, a multifactorial problem related to body fat accumulation that leads to several life-threatening diseases, becoming one of the most critical public health problems worldwide. In this review, we propose to provide the highlights of the adipogenesis study based on in vitro differentiation of human mesenchymal stem cells (hMSCs). We list in silico methods, such as molecular docking for identification of molecular targets, and in vitro approaches, from 2D, more straightforward and applied for screening large libraries of substances, to more representative physiological models, such as 3D and bioprinting models. We also describe the development of physiological models based on microfluidic systems applied to investigate adipogenesis in vitro. We intend to identify the main alternative models for adipogenesis evaluation, contributing to the direction of preclinical research in obesity. Future directions indicate the association of in silico and in vitro techniques to bring a clear picture of alternative methods based on adipogenesis as a tool for obesity research. |
format | Online Article Text |
id | pubmed-9860640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98606402023-01-22 Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches Pamplona, Juliana Helena Zoehler, Bernardo Shigunov, Patrícia Barisón, María Julia Severo, Vanessa Rossini Erich, Natalie Mayara Ribeiro, Annanda Lyra Horinouchi, Cintia Delai da Silva Suzukawa, Andréia Akemi Robert, Anny Waloski Abud, Ana Paula Ressetti de Aguiar, Alessandra Melo Life (Basel) Review The study of adipogenesis is essential for understanding and treating obesity, a multifactorial problem related to body fat accumulation that leads to several life-threatening diseases, becoming one of the most critical public health problems worldwide. In this review, we propose to provide the highlights of the adipogenesis study based on in vitro differentiation of human mesenchymal stem cells (hMSCs). We list in silico methods, such as molecular docking for identification of molecular targets, and in vitro approaches, from 2D, more straightforward and applied for screening large libraries of substances, to more representative physiological models, such as 3D and bioprinting models. We also describe the development of physiological models based on microfluidic systems applied to investigate adipogenesis in vitro. We intend to identify the main alternative models for adipogenesis evaluation, contributing to the direction of preclinical research in obesity. Future directions indicate the association of in silico and in vitro techniques to bring a clear picture of alternative methods based on adipogenesis as a tool for obesity research. MDPI 2022-12-30 /pmc/articles/PMC9860640/ /pubmed/36676057 http://dx.doi.org/10.3390/life13010108 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pamplona, Juliana Helena Zoehler, Bernardo Shigunov, Patrícia Barisón, María Julia Severo, Vanessa Rossini Erich, Natalie Mayara Ribeiro, Annanda Lyra Horinouchi, Cintia Delai da Silva Suzukawa, Andréia Akemi Robert, Anny Waloski Abud, Ana Paula Ressetti de Aguiar, Alessandra Melo Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches |
title | Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches |
title_full | Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches |
title_fullStr | Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches |
title_full_unstemmed | Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches |
title_short | Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches |
title_sort | alternative methods as tools for obesity research: in vitro and in silico approaches |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860640/ https://www.ncbi.nlm.nih.gov/pubmed/36676057 http://dx.doi.org/10.3390/life13010108 |
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