Cargando…
Alterations in Renin–Angiotensin System (RAS) Peptide Levels in Patients with HIV
Chronic HIV infection has long been associated with an increased risk for cardiovascular diseases. The metabolites of the renin–angiotensin system (RAS) such as angiotensin II (AngII) play an important role in regulating blood pressure and fluid dynamics. Cross-sectional analysis of HIV-positive ind...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860813/ https://www.ncbi.nlm.nih.gov/pubmed/36676986 http://dx.doi.org/10.3390/metabo13010061 |
_version_ | 1784874682306002944 |
---|---|
author | Asante, Isaac Lu, Angela Mitchell, Brooks I. Boisvert, William A. Shikuma, Cecilia M. Chow, Dominic C. Louie, Stan G. |
author_facet | Asante, Isaac Lu, Angela Mitchell, Brooks I. Boisvert, William A. Shikuma, Cecilia M. Chow, Dominic C. Louie, Stan G. |
author_sort | Asante, Isaac |
collection | PubMed |
description | Chronic HIV infection has long been associated with an increased risk for cardiovascular diseases. The metabolites of the renin–angiotensin system (RAS) such as angiotensin II (AngII) play an important role in regulating blood pressure and fluid dynamics. Cross-sectional analysis of HIV-positive individuals (n = 71, age > 40 years, stable ART > 3 months with HIV viral load < 50 copies/mL) were compared to a similar HIV seronegative group (n = 72). High-resolution B-mode ultrasound images of the right carotid bifurcation (RBIF) and right common carotid artery (RCCA) were conducted to measure the extent of carotid atherosclerotic vascular disease. Plasma RAS peptide levels were quantified using a liquid chromatography-mass spectrometry-based metabolomics assay. RAS peptide concentrations were compared between persons with HIV and persons without HIV, correlating their association with clinical and cardiac measures. Median precursor peptides (Ang(1-12) and AngI) were significantly higher in the HIV-positive group compared to the HIV-negative. Analyses of the patient subgroup not on antihypertensive medication revealed circulating levels of AngII to be four-fold higher in the HIV-positive subgroup. AngII and TNF-alpha levels were found to have a positive association with RCCA, and AngI/Ang(1-12) ratio and TNF-alpha levels were found to have a positive association with RBIF. In both predictive models, AngIII had a negative association with either RCCA or RBIF, which may be attributed to its ability to bind onto AT2R and thus oppose pro-inflammatory events. These results reveal systemic alterations in RAS as a result of chronic HIV infection, which may lead to the activation of inflammatory pathways associated with carotid thickening. RAS peptide levels and cytokine markers were associated with RCCA and RBIF measurements. |
format | Online Article Text |
id | pubmed-9860813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98608132023-01-22 Alterations in Renin–Angiotensin System (RAS) Peptide Levels in Patients with HIV Asante, Isaac Lu, Angela Mitchell, Brooks I. Boisvert, William A. Shikuma, Cecilia M. Chow, Dominic C. Louie, Stan G. Metabolites Article Chronic HIV infection has long been associated with an increased risk for cardiovascular diseases. The metabolites of the renin–angiotensin system (RAS) such as angiotensin II (AngII) play an important role in regulating blood pressure and fluid dynamics. Cross-sectional analysis of HIV-positive individuals (n = 71, age > 40 years, stable ART > 3 months with HIV viral load < 50 copies/mL) were compared to a similar HIV seronegative group (n = 72). High-resolution B-mode ultrasound images of the right carotid bifurcation (RBIF) and right common carotid artery (RCCA) were conducted to measure the extent of carotid atherosclerotic vascular disease. Plasma RAS peptide levels were quantified using a liquid chromatography-mass spectrometry-based metabolomics assay. RAS peptide concentrations were compared between persons with HIV and persons without HIV, correlating their association with clinical and cardiac measures. Median precursor peptides (Ang(1-12) and AngI) were significantly higher in the HIV-positive group compared to the HIV-negative. Analyses of the patient subgroup not on antihypertensive medication revealed circulating levels of AngII to be four-fold higher in the HIV-positive subgroup. AngII and TNF-alpha levels were found to have a positive association with RCCA, and AngI/Ang(1-12) ratio and TNF-alpha levels were found to have a positive association with RBIF. In both predictive models, AngIII had a negative association with either RCCA or RBIF, which may be attributed to its ability to bind onto AT2R and thus oppose pro-inflammatory events. These results reveal systemic alterations in RAS as a result of chronic HIV infection, which may lead to the activation of inflammatory pathways associated with carotid thickening. RAS peptide levels and cytokine markers were associated with RCCA and RBIF measurements. MDPI 2022-12-31 /pmc/articles/PMC9860813/ /pubmed/36676986 http://dx.doi.org/10.3390/metabo13010061 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Asante, Isaac Lu, Angela Mitchell, Brooks I. Boisvert, William A. Shikuma, Cecilia M. Chow, Dominic C. Louie, Stan G. Alterations in Renin–Angiotensin System (RAS) Peptide Levels in Patients with HIV |
title | Alterations in Renin–Angiotensin System (RAS) Peptide Levels in Patients with HIV |
title_full | Alterations in Renin–Angiotensin System (RAS) Peptide Levels in Patients with HIV |
title_fullStr | Alterations in Renin–Angiotensin System (RAS) Peptide Levels in Patients with HIV |
title_full_unstemmed | Alterations in Renin–Angiotensin System (RAS) Peptide Levels in Patients with HIV |
title_short | Alterations in Renin–Angiotensin System (RAS) Peptide Levels in Patients with HIV |
title_sort | alterations in renin–angiotensin system (ras) peptide levels in patients with hiv |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860813/ https://www.ncbi.nlm.nih.gov/pubmed/36676986 http://dx.doi.org/10.3390/metabo13010061 |
work_keys_str_mv | AT asanteisaac alterationsinreninangiotensinsystemraspeptidelevelsinpatientswithhiv AT luangela alterationsinreninangiotensinsystemraspeptidelevelsinpatientswithhiv AT mitchellbrooksi alterationsinreninangiotensinsystemraspeptidelevelsinpatientswithhiv AT boisvertwilliama alterationsinreninangiotensinsystemraspeptidelevelsinpatientswithhiv AT shikumaceciliam alterationsinreninangiotensinsystemraspeptidelevelsinpatientswithhiv AT chowdominicc alterationsinreninangiotensinsystemraspeptidelevelsinpatientswithhiv AT louiestang alterationsinreninangiotensinsystemraspeptidelevelsinpatientswithhiv |