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Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota

Maternal betaine supplementation has been proven to alleviate non-alcoholic fatty liver disease (NAFLD) in offspring caused by maternal high-fat diet (MHFD). The gut–liver axis plays an important role in NAFLD pathogenesis. However, whether maternal betaine supplementation can alleviate NAFLD in off...

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Autores principales: Sun, Liuqiao, Tan, Xuying, Liang, Xiaoping, Chen, Hangjun, Ou, Qian, Wu, Qiongmei, Yu, Xinxue, Zhao, Hanqing, Huang, Qiaoli, Yi, Zehua, Wei, Jun, Wu, Feng, Zhu, Huilian, Wang, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861146/
https://www.ncbi.nlm.nih.gov/pubmed/36678155
http://dx.doi.org/10.3390/nu15020284
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author Sun, Liuqiao
Tan, Xuying
Liang, Xiaoping
Chen, Hangjun
Ou, Qian
Wu, Qiongmei
Yu, Xinxue
Zhao, Hanqing
Huang, Qiaoli
Yi, Zehua
Wei, Jun
Wu, Feng
Zhu, Huilian
Wang, Lijun
author_facet Sun, Liuqiao
Tan, Xuying
Liang, Xiaoping
Chen, Hangjun
Ou, Qian
Wu, Qiongmei
Yu, Xinxue
Zhao, Hanqing
Huang, Qiaoli
Yi, Zehua
Wei, Jun
Wu, Feng
Zhu, Huilian
Wang, Lijun
author_sort Sun, Liuqiao
collection PubMed
description Maternal betaine supplementation has been proven to alleviate non-alcoholic fatty liver disease (NAFLD) in offspring caused by maternal high-fat diet (MHFD). The gut–liver axis plays an important role in NAFLD pathogenesis. However, whether maternal betaine supplementation can alleviate NAFLD in offspring by the gut–liver axis is unknown. C57BL/6J mice were fed with high-fat diet for 4 weeks before mating, and supplemented with 1% betaine during pregnancy and lactation. After weaning, offspring mice were fed with standard diet to 10 weeks. Maternal betaine supplementation reduced hepatic triglyceride content and alleviated hepatic steatosis in offspring mice exposed to MHFD. Furthermore, the mRNA expression of PPARα, CPT1α and FATP2 was increased and TNFα was reduced by maternal betaine supplementation. Maternal betaine intake decreased the relative abundances of Proteobateria, Desulfovibrio and Ruminococcus, but increased the relative abundances of Bacteroides and Parabacteroides. Moreover, maternal betaine intake increased the concentrations of short-chain fatty acids (SCFAs), including acetic acid, butyric acid and valeric acid, in the feces. Gut microbiota and SCFAs were significantly correlated with hepatic triglyceride content and expression of the above genes. Maternal betaine intake had no effect on other gut microbiota-related metabolites (bile acid and trimethylamine-n-oxide). Altogether, maternal betaine supplementation ameliorated MHFD-induced NAFLD possibly through regulating gut microbiota and SCFAs in offspring mice.
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spelling pubmed-98611462023-01-22 Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota Sun, Liuqiao Tan, Xuying Liang, Xiaoping Chen, Hangjun Ou, Qian Wu, Qiongmei Yu, Xinxue Zhao, Hanqing Huang, Qiaoli Yi, Zehua Wei, Jun Wu, Feng Zhu, Huilian Wang, Lijun Nutrients Article Maternal betaine supplementation has been proven to alleviate non-alcoholic fatty liver disease (NAFLD) in offspring caused by maternal high-fat diet (MHFD). The gut–liver axis plays an important role in NAFLD pathogenesis. However, whether maternal betaine supplementation can alleviate NAFLD in offspring by the gut–liver axis is unknown. C57BL/6J mice were fed with high-fat diet for 4 weeks before mating, and supplemented with 1% betaine during pregnancy and lactation. After weaning, offspring mice were fed with standard diet to 10 weeks. Maternal betaine supplementation reduced hepatic triglyceride content and alleviated hepatic steatosis in offspring mice exposed to MHFD. Furthermore, the mRNA expression of PPARα, CPT1α and FATP2 was increased and TNFα was reduced by maternal betaine supplementation. Maternal betaine intake decreased the relative abundances of Proteobateria, Desulfovibrio and Ruminococcus, but increased the relative abundances of Bacteroides and Parabacteroides. Moreover, maternal betaine intake increased the concentrations of short-chain fatty acids (SCFAs), including acetic acid, butyric acid and valeric acid, in the feces. Gut microbiota and SCFAs were significantly correlated with hepatic triglyceride content and expression of the above genes. Maternal betaine intake had no effect on other gut microbiota-related metabolites (bile acid and trimethylamine-n-oxide). Altogether, maternal betaine supplementation ameliorated MHFD-induced NAFLD possibly through regulating gut microbiota and SCFAs in offspring mice. MDPI 2023-01-06 /pmc/articles/PMC9861146/ /pubmed/36678155 http://dx.doi.org/10.3390/nu15020284 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Liuqiao
Tan, Xuying
Liang, Xiaoping
Chen, Hangjun
Ou, Qian
Wu, Qiongmei
Yu, Xinxue
Zhao, Hanqing
Huang, Qiaoli
Yi, Zehua
Wei, Jun
Wu, Feng
Zhu, Huilian
Wang, Lijun
Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota
title Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota
title_full Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota
title_fullStr Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota
title_full_unstemmed Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota
title_short Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota
title_sort maternal betaine supplementation mitigates maternal high fat diet-induced nafld in offspring mice through gut microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861146/
https://www.ncbi.nlm.nih.gov/pubmed/36678155
http://dx.doi.org/10.3390/nu15020284
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