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Effectiveness of Flattening-Filter-Free versus Flattened Beams in V79 and Glioblastoma Patient-Derived Stem-like Cells

Literature data on the administration of conventional high-dose beams with (FF) or without flattening filters (FFF) show conflicting results on biological effects at the cellular level. To contribute to this field, we irradiated V79 Chinese hamster lung fibroblasts and two patient-derived glioblasto...

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Autores principales: Dini, Valentina, Esposito, Giuseppe, Sacconi, Andrea, D’Andrea, Marco, Tabocchini, Maria Antonella, Anello, Pasquale, Ricci-Vitiani, Lucia, Buccarelli, Mariachiara, Pallini, Roberto, Strigari, Lidia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861147/
https://www.ncbi.nlm.nih.gov/pubmed/36674623
http://dx.doi.org/10.3390/ijms24021107
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author Dini, Valentina
Esposito, Giuseppe
Sacconi, Andrea
D’Andrea, Marco
Tabocchini, Maria Antonella
Anello, Pasquale
Ricci-Vitiani, Lucia
Buccarelli, Mariachiara
Pallini, Roberto
Strigari, Lidia
author_facet Dini, Valentina
Esposito, Giuseppe
Sacconi, Andrea
D’Andrea, Marco
Tabocchini, Maria Antonella
Anello, Pasquale
Ricci-Vitiani, Lucia
Buccarelli, Mariachiara
Pallini, Roberto
Strigari, Lidia
author_sort Dini, Valentina
collection PubMed
description Literature data on the administration of conventional high-dose beams with (FF) or without flattening filters (FFF) show conflicting results on biological effects at the cellular level. To contribute to this field, we irradiated V79 Chinese hamster lung fibroblasts and two patient-derived glioblastoma stem-like cell lines (GSCs—named #1 and #83) using a clinical 10 MV accelerator with FF (at 4 Gy/min) and FFF (at two dose rates 4 and 24 Gy/min). Cell killing and DNA damage induction, determined using the γ-H2AX assay, and gene expression were studied. No significant differences in the early survival of V79 cells were observed as a function of dose rates and FF or FFF beams, while a trend of reduction in late survival was observed at the highest dose rate with the FFF beam. GSCs showed similar survival levels as a function of dose rates, both delivered in the FFF regimen. The amount of DNA damage measured for both dose rates after 2 h was much higher in line #1 than in line #83, with statistically significant differences between the two dose rates only in line #83. The gene expression analysis of the two GSC lines indicates gene signatures mimicking the prognosis of glioblastoma (GBM) patients derived from a public database. Overall, the results support the current use of FFF and highlight the possibility of identifying patients with candidate gene signatures that could benefit from irradiation with FFF beams at a high dose rate.
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spelling pubmed-98611472023-01-22 Effectiveness of Flattening-Filter-Free versus Flattened Beams in V79 and Glioblastoma Patient-Derived Stem-like Cells Dini, Valentina Esposito, Giuseppe Sacconi, Andrea D’Andrea, Marco Tabocchini, Maria Antonella Anello, Pasquale Ricci-Vitiani, Lucia Buccarelli, Mariachiara Pallini, Roberto Strigari, Lidia Int J Mol Sci Article Literature data on the administration of conventional high-dose beams with (FF) or without flattening filters (FFF) show conflicting results on biological effects at the cellular level. To contribute to this field, we irradiated V79 Chinese hamster lung fibroblasts and two patient-derived glioblastoma stem-like cell lines (GSCs—named #1 and #83) using a clinical 10 MV accelerator with FF (at 4 Gy/min) and FFF (at two dose rates 4 and 24 Gy/min). Cell killing and DNA damage induction, determined using the γ-H2AX assay, and gene expression were studied. No significant differences in the early survival of V79 cells were observed as a function of dose rates and FF or FFF beams, while a trend of reduction in late survival was observed at the highest dose rate with the FFF beam. GSCs showed similar survival levels as a function of dose rates, both delivered in the FFF regimen. The amount of DNA damage measured for both dose rates after 2 h was much higher in line #1 than in line #83, with statistically significant differences between the two dose rates only in line #83. The gene expression analysis of the two GSC lines indicates gene signatures mimicking the prognosis of glioblastoma (GBM) patients derived from a public database. Overall, the results support the current use of FFF and highlight the possibility of identifying patients with candidate gene signatures that could benefit from irradiation with FFF beams at a high dose rate. MDPI 2023-01-06 /pmc/articles/PMC9861147/ /pubmed/36674623 http://dx.doi.org/10.3390/ijms24021107 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dini, Valentina
Esposito, Giuseppe
Sacconi, Andrea
D’Andrea, Marco
Tabocchini, Maria Antonella
Anello, Pasquale
Ricci-Vitiani, Lucia
Buccarelli, Mariachiara
Pallini, Roberto
Strigari, Lidia
Effectiveness of Flattening-Filter-Free versus Flattened Beams in V79 and Glioblastoma Patient-Derived Stem-like Cells
title Effectiveness of Flattening-Filter-Free versus Flattened Beams in V79 and Glioblastoma Patient-Derived Stem-like Cells
title_full Effectiveness of Flattening-Filter-Free versus Flattened Beams in V79 and Glioblastoma Patient-Derived Stem-like Cells
title_fullStr Effectiveness of Flattening-Filter-Free versus Flattened Beams in V79 and Glioblastoma Patient-Derived Stem-like Cells
title_full_unstemmed Effectiveness of Flattening-Filter-Free versus Flattened Beams in V79 and Glioblastoma Patient-Derived Stem-like Cells
title_short Effectiveness of Flattening-Filter-Free versus Flattened Beams in V79 and Glioblastoma Patient-Derived Stem-like Cells
title_sort effectiveness of flattening-filter-free versus flattened beams in v79 and glioblastoma patient-derived stem-like cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861147/
https://www.ncbi.nlm.nih.gov/pubmed/36674623
http://dx.doi.org/10.3390/ijms24021107
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