Cargando…

Genetically Encoded Self-Assembling Protein Nanoparticles for the Targeted Delivery In Vitro and In Vivo

Targeted nanoparticles of different origins are considered as new-generation diagnostic and therapeutic tools. However, there are no targeted drug formulations within the composition of nanoparticles approved by the FDA for use in the clinic, which is associated with the insufficient effectiveness o...

Descripción completa

Detalles Bibliográficos
Autores principales: Obozina, Anastasiia S., Komedchikova, Elena N., Kolesnikova, Olga A., Iureva, Anna M., Kovalenko, Vera L., Zavalko, Fedor A., Rozhnikova, Tatiana V., Tereshina, Ekaterina D., Mochalova, Elizaveta N., Shipunova, Victoria O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861179/
https://www.ncbi.nlm.nih.gov/pubmed/36678860
http://dx.doi.org/10.3390/pharmaceutics15010231
_version_ 1784874776978784256
author Obozina, Anastasiia S.
Komedchikova, Elena N.
Kolesnikova, Olga A.
Iureva, Anna M.
Kovalenko, Vera L.
Zavalko, Fedor A.
Rozhnikova, Tatiana V.
Tereshina, Ekaterina D.
Mochalova, Elizaveta N.
Shipunova, Victoria O.
author_facet Obozina, Anastasiia S.
Komedchikova, Elena N.
Kolesnikova, Olga A.
Iureva, Anna M.
Kovalenko, Vera L.
Zavalko, Fedor A.
Rozhnikova, Tatiana V.
Tereshina, Ekaterina D.
Mochalova, Elizaveta N.
Shipunova, Victoria O.
author_sort Obozina, Anastasiia S.
collection PubMed
description Targeted nanoparticles of different origins are considered as new-generation diagnostic and therapeutic tools. However, there are no targeted drug formulations within the composition of nanoparticles approved by the FDA for use in the clinic, which is associated with the insufficient effectiveness of the developed candidates, the difficulties of their biotechnological production, and inadequate batch-to-batch reproducibility. Targeted protein self-assembling nanoparticles circumvent this problem since proteins are encoded in DNA and the final protein product is produced in only one possible way. We believe that the combination of the endless biomedical potential of protein carriers as nanoparticles and the standardized protein purification protocols will make significant progress in “magic bullet” creation possible, bringing modern biomedicine to a new level. In this review, we are focused on the currently existing platforms for targeted self-assembling protein nanoparticles based on transferrin, lactoferrin, casein, lumazine synthase, albumin, ferritin, and encapsulin proteins, as well as on proteins from magnetosomes and virus-like particles. The applications of these self-assembling proteins for targeted delivery in vitro and in vivo are thoroughly discussed, including bioimaging applications and different therapeutic approaches, such as chemotherapy, gene delivery, and photodynamic and photothermal therapy. A critical assessment of these protein platforms’ efficacy in biomedicine is provided and possible problems associated with their further development are described.
format Online
Article
Text
id pubmed-9861179
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98611792023-01-22 Genetically Encoded Self-Assembling Protein Nanoparticles for the Targeted Delivery In Vitro and In Vivo Obozina, Anastasiia S. Komedchikova, Elena N. Kolesnikova, Olga A. Iureva, Anna M. Kovalenko, Vera L. Zavalko, Fedor A. Rozhnikova, Tatiana V. Tereshina, Ekaterina D. Mochalova, Elizaveta N. Shipunova, Victoria O. Pharmaceutics Review Targeted nanoparticles of different origins are considered as new-generation diagnostic and therapeutic tools. However, there are no targeted drug formulations within the composition of nanoparticles approved by the FDA for use in the clinic, which is associated with the insufficient effectiveness of the developed candidates, the difficulties of their biotechnological production, and inadequate batch-to-batch reproducibility. Targeted protein self-assembling nanoparticles circumvent this problem since proteins are encoded in DNA and the final protein product is produced in only one possible way. We believe that the combination of the endless biomedical potential of protein carriers as nanoparticles and the standardized protein purification protocols will make significant progress in “magic bullet” creation possible, bringing modern biomedicine to a new level. In this review, we are focused on the currently existing platforms for targeted self-assembling protein nanoparticles based on transferrin, lactoferrin, casein, lumazine synthase, albumin, ferritin, and encapsulin proteins, as well as on proteins from magnetosomes and virus-like particles. The applications of these self-assembling proteins for targeted delivery in vitro and in vivo are thoroughly discussed, including bioimaging applications and different therapeutic approaches, such as chemotherapy, gene delivery, and photodynamic and photothermal therapy. A critical assessment of these protein platforms’ efficacy in biomedicine is provided and possible problems associated with their further development are described. MDPI 2023-01-10 /pmc/articles/PMC9861179/ /pubmed/36678860 http://dx.doi.org/10.3390/pharmaceutics15010231 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Obozina, Anastasiia S.
Komedchikova, Elena N.
Kolesnikova, Olga A.
Iureva, Anna M.
Kovalenko, Vera L.
Zavalko, Fedor A.
Rozhnikova, Tatiana V.
Tereshina, Ekaterina D.
Mochalova, Elizaveta N.
Shipunova, Victoria O.
Genetically Encoded Self-Assembling Protein Nanoparticles for the Targeted Delivery In Vitro and In Vivo
title Genetically Encoded Self-Assembling Protein Nanoparticles for the Targeted Delivery In Vitro and In Vivo
title_full Genetically Encoded Self-Assembling Protein Nanoparticles for the Targeted Delivery In Vitro and In Vivo
title_fullStr Genetically Encoded Self-Assembling Protein Nanoparticles for the Targeted Delivery In Vitro and In Vivo
title_full_unstemmed Genetically Encoded Self-Assembling Protein Nanoparticles for the Targeted Delivery In Vitro and In Vivo
title_short Genetically Encoded Self-Assembling Protein Nanoparticles for the Targeted Delivery In Vitro and In Vivo
title_sort genetically encoded self-assembling protein nanoparticles for the targeted delivery in vitro and in vivo
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861179/
https://www.ncbi.nlm.nih.gov/pubmed/36678860
http://dx.doi.org/10.3390/pharmaceutics15010231
work_keys_str_mv AT obozinaanastasiias geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT komedchikovaelenan geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT kolesnikovaolgaa geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT iurevaannam geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT kovalenkoveral geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT zavalkofedora geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT rozhnikovatatianav geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT tereshinaekaterinad geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT mochalovaelizavetan geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo
AT shipunovavictoriao geneticallyencodedselfassemblingproteinnanoparticlesforthetargeteddeliveryinvitroandinvivo