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In Vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid
The Cattleya (Orchidaceae–Laeliinae subtribe) intergeneric hybrids, such as Brassolaeliocattleya (Blc.), have great ornamental value, due to their compact-size, with large and high color diversity of flowers. Artificial induction of polyploidy brings agronomic, ornamental and genetic benefits to pla...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861230/ https://www.ncbi.nlm.nih.gov/pubmed/36678995 http://dx.doi.org/10.3390/plants12020281 |
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author | Vilcherrez-Atoche, Joe Abdul Silva, Jéssica Coutinho Clarindo, Wellington Ronildo Mondin, Mateus Cardoso, Jean Carlos |
author_facet | Vilcherrez-Atoche, Joe Abdul Silva, Jéssica Coutinho Clarindo, Wellington Ronildo Mondin, Mateus Cardoso, Jean Carlos |
author_sort | Vilcherrez-Atoche, Joe Abdul |
collection | PubMed |
description | The Cattleya (Orchidaceae–Laeliinae subtribe) intergeneric hybrids, such as Brassolaeliocattleya (Blc.), have great ornamental value, due to their compact-size, with large and high color diversity of flowers. Artificial induction of polyploidy brings agronomic, ornamental and genetic benefits to plants. Polyploidization efficiency depends on factors, such as the type of antimitotic, polyploidization method, concentrations, exposure times and type of explant. This study aimed to develop a protocol to polyploidize Blc. orchids, by testing two types of explants (seeds and protocorms), concentrations and exposure times to colchicine. The effects of colchicine on the in vitro development of explants were also investigated. The responses of explants to colchicine depended on the concentrations, exposure time and the interaction of these factors. Flow cytometric analysis evidenced high endopolyploidy and allowed the separation of polyploidized (4C, 8C and 16C peaks) from non-polyploidized (only 2C and 4C peaks) plants. The highest percentage of polyploid plants was regenerated from protocorms (16.4%) treated with colchicine instead of seeds (3.2%). Protocorms treated with colchicine at 500–750 μM for 18 h resulted in the best percentage of polyploidization. Additionally, in vitro natural polyploidization using protocorms was reported (11.5%). Cytological analyses allowed an estimation of the number of chromosomes of the parents (≡70), polyploidized (≡140) and non-polyploidized progeny (≡70). |
format | Online Article Text |
id | pubmed-9861230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98612302023-01-22 In Vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid Vilcherrez-Atoche, Joe Abdul Silva, Jéssica Coutinho Clarindo, Wellington Ronildo Mondin, Mateus Cardoso, Jean Carlos Plants (Basel) Article The Cattleya (Orchidaceae–Laeliinae subtribe) intergeneric hybrids, such as Brassolaeliocattleya (Blc.), have great ornamental value, due to their compact-size, with large and high color diversity of flowers. Artificial induction of polyploidy brings agronomic, ornamental and genetic benefits to plants. Polyploidization efficiency depends on factors, such as the type of antimitotic, polyploidization method, concentrations, exposure times and type of explant. This study aimed to develop a protocol to polyploidize Blc. orchids, by testing two types of explants (seeds and protocorms), concentrations and exposure times to colchicine. The effects of colchicine on the in vitro development of explants were also investigated. The responses of explants to colchicine depended on the concentrations, exposure time and the interaction of these factors. Flow cytometric analysis evidenced high endopolyploidy and allowed the separation of polyploidized (4C, 8C and 16C peaks) from non-polyploidized (only 2C and 4C peaks) plants. The highest percentage of polyploid plants was regenerated from protocorms (16.4%) treated with colchicine instead of seeds (3.2%). Protocorms treated with colchicine at 500–750 μM for 18 h resulted in the best percentage of polyploidization. Additionally, in vitro natural polyploidization using protocorms was reported (11.5%). Cytological analyses allowed an estimation of the number of chromosomes of the parents (≡70), polyploidized (≡140) and non-polyploidized progeny (≡70). MDPI 2023-01-07 /pmc/articles/PMC9861230/ /pubmed/36678995 http://dx.doi.org/10.3390/plants12020281 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vilcherrez-Atoche, Joe Abdul Silva, Jéssica Coutinho Clarindo, Wellington Ronildo Mondin, Mateus Cardoso, Jean Carlos In Vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid |
title | In Vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid |
title_full | In Vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid |
title_fullStr | In Vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid |
title_full_unstemmed | In Vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid |
title_short | In Vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid |
title_sort | in vitro polyploidization of brassolaeliocattleya hybrid orchid |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861230/ https://www.ncbi.nlm.nih.gov/pubmed/36678995 http://dx.doi.org/10.3390/plants12020281 |
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