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siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition
Skin wounds remain a significant problem for the healthcare system, affecting the clinical outcome, patients’ quality of life, and financial costs. Reduced wound healing times would improve clinical, economic, and social aspects for both patients and the healthcare system. Skin wound healing has bee...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861445/ https://www.ncbi.nlm.nih.gov/pubmed/36674843 http://dx.doi.org/10.3390/ijms24021326 |
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author | Szymanski, Lukasz Lewicki, Sławomir Markiewicz, Tomasz Cierniak, Szczepan Tassan, Jean-Pierre Kubiak, Jacek Z. |
author_facet | Szymanski, Lukasz Lewicki, Sławomir Markiewicz, Tomasz Cierniak, Szczepan Tassan, Jean-Pierre Kubiak, Jacek Z. |
author_sort | Szymanski, Lukasz |
collection | PubMed |
description | Skin wounds remain a significant problem for the healthcare system, affecting the clinical outcome, patients’ quality of life, and financial costs. Reduced wound healing times would improve clinical, economic, and social aspects for both patients and the healthcare system. Skin wound healing has been studied for years, but effective therapy that leads to accelerated wound healing remains to be discovered. This study aimed to evaluate the potential of MELK silencing to accelerate wound healing. A vectorless, transient knockdown of the MELK gene using siRNA was performed in a murine skin wound model. The wound size, total collagen, type 3 collagen, vessel size, vessel number, cell proliferation, cell apoptosis, number of mast cells, and immune infiltration by CD45, CD11b, CD45, and CD8a cells were evaluated. We observed that treatment with MELK siRNA leads to significantly faster wound closing associated with increased collagen deposition. |
format | Online Article Text |
id | pubmed-9861445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98614452023-01-22 siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition Szymanski, Lukasz Lewicki, Sławomir Markiewicz, Tomasz Cierniak, Szczepan Tassan, Jean-Pierre Kubiak, Jacek Z. Int J Mol Sci Article Skin wounds remain a significant problem for the healthcare system, affecting the clinical outcome, patients’ quality of life, and financial costs. Reduced wound healing times would improve clinical, economic, and social aspects for both patients and the healthcare system. Skin wound healing has been studied for years, but effective therapy that leads to accelerated wound healing remains to be discovered. This study aimed to evaluate the potential of MELK silencing to accelerate wound healing. A vectorless, transient knockdown of the MELK gene using siRNA was performed in a murine skin wound model. The wound size, total collagen, type 3 collagen, vessel size, vessel number, cell proliferation, cell apoptosis, number of mast cells, and immune infiltration by CD45, CD11b, CD45, and CD8a cells were evaluated. We observed that treatment with MELK siRNA leads to significantly faster wound closing associated with increased collagen deposition. MDPI 2023-01-10 /pmc/articles/PMC9861445/ /pubmed/36674843 http://dx.doi.org/10.3390/ijms24021326 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Szymanski, Lukasz Lewicki, Sławomir Markiewicz, Tomasz Cierniak, Szczepan Tassan, Jean-Pierre Kubiak, Jacek Z. siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition |
title | siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition |
title_full | siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition |
title_fullStr | siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition |
title_full_unstemmed | siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition |
title_short | siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition |
title_sort | sirna-mediated melk knockdown induces accelerated wound healing with increased collagen deposition |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861445/ https://www.ncbi.nlm.nih.gov/pubmed/36674843 http://dx.doi.org/10.3390/ijms24021326 |
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