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Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line
Opioid drugs have analgesic properties used to treat chronic and post-surgical pain due to descending pain modulation. The use of opioids is often associated with adverse effects or clinical issues. This study aimed to evaluate the toxicity of opioids by exposing the neuroblastoma cell line (SH-SY5Y...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861468/ https://www.ncbi.nlm.nih.gov/pubmed/36674961 http://dx.doi.org/10.3390/ijms24021424 |
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author | Lima, Luíza Siqueira da Costa, Nayara de Souza Galiciolli, Maria Eduarda Andrade Pereira, Meire Ellen Almeida, William Margarete Cestari, Marta Nogara, Pablo Andrei Irioda, Ana Carolina Oliveira, Cláudia Sirlene |
author_facet | Lima, Luíza Siqueira da Costa, Nayara de Souza Galiciolli, Maria Eduarda Andrade Pereira, Meire Ellen Almeida, William Margarete Cestari, Marta Nogara, Pablo Andrei Irioda, Ana Carolina Oliveira, Cláudia Sirlene |
author_sort | Lima, Luíza Siqueira |
collection | PubMed |
description | Opioid drugs have analgesic properties used to treat chronic and post-surgical pain due to descending pain modulation. The use of opioids is often associated with adverse effects or clinical issues. This study aimed to evaluate the toxicity of opioids by exposing the neuroblastoma cell line (SH-SY5Y) to 0, 1, 10, and 100 µM oxycodone and naloxone for 24 h. Analyses were carried out to evaluate cell cytotoxicity, identification of cell death, DNA damage, superoxide dismutase (SOD), glutathione S-transferase (GST), and acetylcholinesterase (AChE) activities, in addition to molecular docking. Oxycodone and naloxone exposure did not alter the SH-SY5Y cell viability. The exposure to 100 µM oxycodone and naloxone significantly increased the cells’ DNA damage score compared to the control group. Naloxone exposure significantly inhibited AChE, GST, and SOD activities, while oxycodone did not alter these enzymes’ activities. Molecular docking showed that naloxone and oxycodone interact with different amino acids in the studied enzymes, which may explain the differences in enzymatic inhibition. Naloxone altered the antioxidant defenses of SH-SY5Y cells, which may have caused DNA damage 24 h after the exposure. On the other hand, more studies are necessary to explain how oxycodone causes DNA damage. |
format | Online Article Text |
id | pubmed-9861468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98614682023-01-22 Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line Lima, Luíza Siqueira da Costa, Nayara de Souza Galiciolli, Maria Eduarda Andrade Pereira, Meire Ellen Almeida, William Margarete Cestari, Marta Nogara, Pablo Andrei Irioda, Ana Carolina Oliveira, Cláudia Sirlene Int J Mol Sci Article Opioid drugs have analgesic properties used to treat chronic and post-surgical pain due to descending pain modulation. The use of opioids is often associated with adverse effects or clinical issues. This study aimed to evaluate the toxicity of opioids by exposing the neuroblastoma cell line (SH-SY5Y) to 0, 1, 10, and 100 µM oxycodone and naloxone for 24 h. Analyses were carried out to evaluate cell cytotoxicity, identification of cell death, DNA damage, superoxide dismutase (SOD), glutathione S-transferase (GST), and acetylcholinesterase (AChE) activities, in addition to molecular docking. Oxycodone and naloxone exposure did not alter the SH-SY5Y cell viability. The exposure to 100 µM oxycodone and naloxone significantly increased the cells’ DNA damage score compared to the control group. Naloxone exposure significantly inhibited AChE, GST, and SOD activities, while oxycodone did not alter these enzymes’ activities. Molecular docking showed that naloxone and oxycodone interact with different amino acids in the studied enzymes, which may explain the differences in enzymatic inhibition. Naloxone altered the antioxidant defenses of SH-SY5Y cells, which may have caused DNA damage 24 h after the exposure. On the other hand, more studies are necessary to explain how oxycodone causes DNA damage. MDPI 2023-01-11 /pmc/articles/PMC9861468/ /pubmed/36674961 http://dx.doi.org/10.3390/ijms24021424 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lima, Luíza Siqueira da Costa, Nayara de Souza Galiciolli, Maria Eduarda Andrade Pereira, Meire Ellen Almeida, William Margarete Cestari, Marta Nogara, Pablo Andrei Irioda, Ana Carolina Oliveira, Cláudia Sirlene Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line |
title | Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line |
title_full | Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line |
title_fullStr | Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line |
title_full_unstemmed | Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line |
title_short | Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line |
title_sort | assessment of neurotoxic effects of oxycodone and naloxone in sh-sy5y cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861468/ https://www.ncbi.nlm.nih.gov/pubmed/36674961 http://dx.doi.org/10.3390/ijms24021424 |
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