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Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Aflatoxins (AFs) are fungi secondary metabolites produced by the Aspergillus family. These compounds can enter the food chain through food contamination, representing a risk to human health. Commercial immunoaffinity columns are widely used for the extraction and cleanup of AFs from food samples; ho...

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Autores principales: Palmieri, Sara, Elfadil, Dounia, Fanti, Federico, Della Pelle, Flavio, Sergi, Manuel, Amine, Aziz, Compagnone, Dario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861586/
https://www.ncbi.nlm.nih.gov/pubmed/36677761
http://dx.doi.org/10.3390/molecules28020703
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author Palmieri, Sara
Elfadil, Dounia
Fanti, Federico
Della Pelle, Flavio
Sergi, Manuel
Amine, Aziz
Compagnone, Dario
author_facet Palmieri, Sara
Elfadil, Dounia
Fanti, Federico
Della Pelle, Flavio
Sergi, Manuel
Amine, Aziz
Compagnone, Dario
author_sort Palmieri, Sara
collection PubMed
description Aflatoxins (AFs) are fungi secondary metabolites produced by the Aspergillus family. These compounds can enter the food chain through food contamination, representing a risk to human health. Commercial immunoaffinity columns are widely used for the extraction and cleanup of AFs from food samples; however, their high cost and large solvent consumption create a need for alternative strategies. In this work, an alternative strategy for producing molecularly imprinted polymers (MIPs) was proposed to extract aflatoxins AFB1, AFB2, AFG1, and AFG2 from complex food samples, using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). The MIPs were synthesized via a low-cost and rapid (5 min) sonochemical free-radical polymerization, using 1-hydroxy-2-naphthoic acid as a dummy template. MIPs-based solid phase extraction performance was tested on 17 dietary supplements (vegetables, fruits, and cereals), obtaining appreciable recovery rates (65–90%) and good reproducibility (RSD ≤ 6%, n = 3); the selectivity towards other mycotoxins was proved and the data obtained compared with commercial immunoaffinity columns. The proposed strategy can be considered an alternative affordable approach to the classical immunoaffinity columns, since it is more selective and better performing.
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spelling pubmed-98615862023-01-22 Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food Palmieri, Sara Elfadil, Dounia Fanti, Federico Della Pelle, Flavio Sergi, Manuel Amine, Aziz Compagnone, Dario Molecules Article Aflatoxins (AFs) are fungi secondary metabolites produced by the Aspergillus family. These compounds can enter the food chain through food contamination, representing a risk to human health. Commercial immunoaffinity columns are widely used for the extraction and cleanup of AFs from food samples; however, their high cost and large solvent consumption create a need for alternative strategies. In this work, an alternative strategy for producing molecularly imprinted polymers (MIPs) was proposed to extract aflatoxins AFB1, AFB2, AFG1, and AFG2 from complex food samples, using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). The MIPs were synthesized via a low-cost and rapid (5 min) sonochemical free-radical polymerization, using 1-hydroxy-2-naphthoic acid as a dummy template. MIPs-based solid phase extraction performance was tested on 17 dietary supplements (vegetables, fruits, and cereals), obtaining appreciable recovery rates (65–90%) and good reproducibility (RSD ≤ 6%, n = 3); the selectivity towards other mycotoxins was proved and the data obtained compared with commercial immunoaffinity columns. The proposed strategy can be considered an alternative affordable approach to the classical immunoaffinity columns, since it is more selective and better performing. MDPI 2023-01-10 /pmc/articles/PMC9861586/ /pubmed/36677761 http://dx.doi.org/10.3390/molecules28020703 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Palmieri, Sara
Elfadil, Dounia
Fanti, Federico
Della Pelle, Flavio
Sergi, Manuel
Amine, Aziz
Compagnone, Dario
Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food
title Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food
title_full Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food
title_fullStr Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food
title_full_unstemmed Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food
title_short Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food
title_sort study on molecularly imprinted polymers obtained sonochemically for the determination of aflatoxins in food
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861586/
https://www.ncbi.nlm.nih.gov/pubmed/36677761
http://dx.doi.org/10.3390/molecules28020703
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