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Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis

WRKY transcription factors, as the largest gene family in higher plants, play an important role in various biological processes including growth and development, regulation of secondary metabolites, and stress response. In this study, we performed genome-wide identification and analysis of WRKY tran...

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Autores principales: Mu, Detian, Chen, Wenqiang, Shao, Yingying, Wilson, Iain W., Zhao, Huan, Luo, Zuliang, Lin, Xiaodong, He, Jialong, Zhang, Yuan, Mo, Changming, Qiu, Deyou, Tang, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861706/
https://www.ncbi.nlm.nih.gov/pubmed/36679001
http://dx.doi.org/10.3390/plants12020288
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author Mu, Detian
Chen, Wenqiang
Shao, Yingying
Wilson, Iain W.
Zhao, Huan
Luo, Zuliang
Lin, Xiaodong
He, Jialong
Zhang, Yuan
Mo, Changming
Qiu, Deyou
Tang, Qi
author_facet Mu, Detian
Chen, Wenqiang
Shao, Yingying
Wilson, Iain W.
Zhao, Huan
Luo, Zuliang
Lin, Xiaodong
He, Jialong
Zhang, Yuan
Mo, Changming
Qiu, Deyou
Tang, Qi
author_sort Mu, Detian
collection PubMed
description WRKY transcription factors, as the largest gene family in higher plants, play an important role in various biological processes including growth and development, regulation of secondary metabolites, and stress response. In this study, we performed genome-wide identification and analysis of WRKY transcription factors in S. siamensis. A total of 59 SsWRKY genes were identified that were distributed on all 14 chromosomes, and these were classified into three major groups based on phylogenetic relationships. Each of these groups had similar conserved motifs and gene structures. We compared all the S. siamensis SsWRKY genes with WRKY genes identified from three diverse plant species, and the results implied that segmental duplication and tandem duplication play an important roles in the evolution processes of the WRKY gene family. Promoter region analysis revealed that SsWRKY genes included many cis-acting elements related to plant growth and development, phytohormone response, and both abiotic and biotic stress. Expression profiles originating from the transcriptome database showed expression patterns of these SsWRKY genes in four different tissues and revealed that most genes are expressed in plant roots. Fifteen SsWRKY genes with low-temperature response motifs were surveyed for their gene expression under cold stress, showing that most genes displayed continuous up-regulation during cold treatment. Our study provides a foundation for further study on the function and regulatory mechanism of the SsWRKY gene family.
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spelling pubmed-98617062023-01-22 Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis Mu, Detian Chen, Wenqiang Shao, Yingying Wilson, Iain W. Zhao, Huan Luo, Zuliang Lin, Xiaodong He, Jialong Zhang, Yuan Mo, Changming Qiu, Deyou Tang, Qi Plants (Basel) Article WRKY transcription factors, as the largest gene family in higher plants, play an important role in various biological processes including growth and development, regulation of secondary metabolites, and stress response. In this study, we performed genome-wide identification and analysis of WRKY transcription factors in S. siamensis. A total of 59 SsWRKY genes were identified that were distributed on all 14 chromosomes, and these were classified into three major groups based on phylogenetic relationships. Each of these groups had similar conserved motifs and gene structures. We compared all the S. siamensis SsWRKY genes with WRKY genes identified from three diverse plant species, and the results implied that segmental duplication and tandem duplication play an important roles in the evolution processes of the WRKY gene family. Promoter region analysis revealed that SsWRKY genes included many cis-acting elements related to plant growth and development, phytohormone response, and both abiotic and biotic stress. Expression profiles originating from the transcriptome database showed expression patterns of these SsWRKY genes in four different tissues and revealed that most genes are expressed in plant roots. Fifteen SsWRKY genes with low-temperature response motifs were surveyed for their gene expression under cold stress, showing that most genes displayed continuous up-regulation during cold treatment. Our study provides a foundation for further study on the function and regulatory mechanism of the SsWRKY gene family. MDPI 2023-01-07 /pmc/articles/PMC9861706/ /pubmed/36679001 http://dx.doi.org/10.3390/plants12020288 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mu, Detian
Chen, Wenqiang
Shao, Yingying
Wilson, Iain W.
Zhao, Huan
Luo, Zuliang
Lin, Xiaodong
He, Jialong
Zhang, Yuan
Mo, Changming
Qiu, Deyou
Tang, Qi
Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis
title Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis
title_full Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis
title_fullStr Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis
title_full_unstemmed Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis
title_short Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis
title_sort genome-wide identification and expression analysis of wrky transcription factors in siraitia siamensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861706/
https://www.ncbi.nlm.nih.gov/pubmed/36679001
http://dx.doi.org/10.3390/plants12020288
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