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Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome....
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861885/ https://www.ncbi.nlm.nih.gov/pubmed/36674824 http://dx.doi.org/10.3390/ijms24021310 |
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author | Jacksi, Mevan Schad, Eva Buday, László Tantos, Agnes |
author_facet | Jacksi, Mevan Schad, Eva Buday, László Tantos, Agnes |
author_sort | Jacksi, Mevan |
collection | PubMed |
description | Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome. Loss of Tks4 resulted in the induction of an EMT-like process, with increased motility and overexpression of EMT markers in colorectal carcinoma cells. In this work, we explored the broader effects of deletion of Tks4 on the gene expression pattern of HCT116 colorectal carcinoma cells by transcriptome sequencing of wild-type and Tks4 knockout (KO) cells. We identified several protein coding genes with altered mRNA levels in the Tks4 KO cell line, as well as a set of long non-coding RNAs, and confirmed these changes with quantitative PCR on a selected set of genes. Our results show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling. |
format | Online Article Text |
id | pubmed-9861885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98618852023-01-22 Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells Jacksi, Mevan Schad, Eva Buday, László Tantos, Agnes Int J Mol Sci Article Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome. Loss of Tks4 resulted in the induction of an EMT-like process, with increased motility and overexpression of EMT markers in colorectal carcinoma cells. In this work, we explored the broader effects of deletion of Tks4 on the gene expression pattern of HCT116 colorectal carcinoma cells by transcriptome sequencing of wild-type and Tks4 knockout (KO) cells. We identified several protein coding genes with altered mRNA levels in the Tks4 KO cell line, as well as a set of long non-coding RNAs, and confirmed these changes with quantitative PCR on a selected set of genes. Our results show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling. MDPI 2023-01-09 /pmc/articles/PMC9861885/ /pubmed/36674824 http://dx.doi.org/10.3390/ijms24021310 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jacksi, Mevan Schad, Eva Buday, László Tantos, Agnes Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells |
title | Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells |
title_full | Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells |
title_fullStr | Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells |
title_full_unstemmed | Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells |
title_short | Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells |
title_sort | absence of scaffold protein tks4 disrupts several signaling pathways in colon cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861885/ https://www.ncbi.nlm.nih.gov/pubmed/36674824 http://dx.doi.org/10.3390/ijms24021310 |
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