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Differential Effects of Endocannabinoids on Amyloid-Beta Aggregation and Toxicity

The regulation and metabolism of the endocannabinoid system has received extensive attention for their potential neuroprotective effect in neurodegenerative diseases such as Alzheimer’s disease (AD), which is characterized by amyloid β (Aβ) -induced cell toxicity, inflammation, and oxidative stress....

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Detalles Bibliográficos
Autores principales: Khavandi, Marzie, Rao, Praveen P. N., Beazely, Michael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861930/
https://www.ncbi.nlm.nih.gov/pubmed/36674424
http://dx.doi.org/10.3390/ijms24020911
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author Khavandi, Marzie
Rao, Praveen P. N.
Beazely, Michael A.
author_facet Khavandi, Marzie
Rao, Praveen P. N.
Beazely, Michael A.
author_sort Khavandi, Marzie
collection PubMed
description The regulation and metabolism of the endocannabinoid system has received extensive attention for their potential neuroprotective effect in neurodegenerative diseases such as Alzheimer’s disease (AD), which is characterized by amyloid β (Aβ) -induced cell toxicity, inflammation, and oxidative stress. Using in vitro techniques and two cell lines, the mouse hippocampus-derived HT22 cells and Chinese hamster ovary (CHO) cells expressing human cannabinoid receptor type 1 (CB1), we investigated the ability of endocannabinoids to inhibit Aβ aggregation and protect cells against Aβ toxicity. The present study provides evidence that endocannabinoids N-arachidonoyl ethanol amide (AEA), noladin and O-arachidonoyl ethanolamine (OAE) inhibit Aβ42 aggregation. They were able to provide protection against Aβ42 induced cytotoxicity via receptor-mediated and non-receptor-mediated mechanisms in CB1-CHO and HT22 cells, respectively. The aggregation kinetic experiments demonstrate the anti-Aβ aggregation activity of some endocannabinoids (AEA, noladin). These data demonstrate the potential role and application of endocannabinoids in AD pathology and treatment.
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spelling pubmed-98619302023-01-22 Differential Effects of Endocannabinoids on Amyloid-Beta Aggregation and Toxicity Khavandi, Marzie Rao, Praveen P. N. Beazely, Michael A. Int J Mol Sci Communication The regulation and metabolism of the endocannabinoid system has received extensive attention for their potential neuroprotective effect in neurodegenerative diseases such as Alzheimer’s disease (AD), which is characterized by amyloid β (Aβ) -induced cell toxicity, inflammation, and oxidative stress. Using in vitro techniques and two cell lines, the mouse hippocampus-derived HT22 cells and Chinese hamster ovary (CHO) cells expressing human cannabinoid receptor type 1 (CB1), we investigated the ability of endocannabinoids to inhibit Aβ aggregation and protect cells against Aβ toxicity. The present study provides evidence that endocannabinoids N-arachidonoyl ethanol amide (AEA), noladin and O-arachidonoyl ethanolamine (OAE) inhibit Aβ42 aggregation. They were able to provide protection against Aβ42 induced cytotoxicity via receptor-mediated and non-receptor-mediated mechanisms in CB1-CHO and HT22 cells, respectively. The aggregation kinetic experiments demonstrate the anti-Aβ aggregation activity of some endocannabinoids (AEA, noladin). These data demonstrate the potential role and application of endocannabinoids in AD pathology and treatment. MDPI 2023-01-04 /pmc/articles/PMC9861930/ /pubmed/36674424 http://dx.doi.org/10.3390/ijms24020911 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Khavandi, Marzie
Rao, Praveen P. N.
Beazely, Michael A.
Differential Effects of Endocannabinoids on Amyloid-Beta Aggregation and Toxicity
title Differential Effects of Endocannabinoids on Amyloid-Beta Aggregation and Toxicity
title_full Differential Effects of Endocannabinoids on Amyloid-Beta Aggregation and Toxicity
title_fullStr Differential Effects of Endocannabinoids on Amyloid-Beta Aggregation and Toxicity
title_full_unstemmed Differential Effects of Endocannabinoids on Amyloid-Beta Aggregation and Toxicity
title_short Differential Effects of Endocannabinoids on Amyloid-Beta Aggregation and Toxicity
title_sort differential effects of endocannabinoids on amyloid-beta aggregation and toxicity
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861930/
https://www.ncbi.nlm.nih.gov/pubmed/36674424
http://dx.doi.org/10.3390/ijms24020911
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