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Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber

Among various available 3D bioprinting techniques, extrusion-based three-dimensional (3D) bioprinting allows the deposition of cell-laden bioink, ensuring predefined scaffold architecture that may offer living tissue regeneration. With a combination of unique characteristics such as biocompatibility...

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Detalles Bibliográficos
Autores principales: Tuladhar, Slesha, Clark, Scott, Habib, Ahasan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861940/
https://www.ncbi.nlm.nih.gov/pubmed/36676319
http://dx.doi.org/10.3390/ma16020572
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author Tuladhar, Slesha
Clark, Scott
Habib, Ahasan
author_facet Tuladhar, Slesha
Clark, Scott
Habib, Ahasan
author_sort Tuladhar, Slesha
collection PubMed
description Among various available 3D bioprinting techniques, extrusion-based three-dimensional (3D) bioprinting allows the deposition of cell-laden bioink, ensuring predefined scaffold architecture that may offer living tissue regeneration. With a combination of unique characteristics such as biocompatibility, less cell toxicity, and high water content, natural hydrogels are a great candidate for bioink formulation for the extrusion-based 3D bioprinting process. However, due to its low mechanical integrity, hydrogel faces a common challenge in maintaining structural integrity. To tackle this challenge, the rheological properties, specifically the shear thinning behavior (reduction of viscosity with increasing the applied load/shear rate on hydrogels) of a set of hybrid hydrogels composed of cellulose-derived nanofiber (TEMPO-mediated nano-fibrillated cellulose, TO-NFC), carboxymethyl cellulose (CMC), and commonly used alginate, were explored. A total of 46 compositions were prepared using higher (0.5% and 1.0%) and lower percentages (0.005% and 0.01%) of TO-NFC, 1–4% of CMC, and 1–4% of alginate to analyze the shear thinning factors such as the values of n and K, which were determined for each composition from the flow diagram and co-related with the 3D printability. The ability to tune shear thinning factors with various ratios of a nanofiber can help achieve a 3D bio-printed scaffold with defined scaffold architecture.
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spelling pubmed-98619402023-01-22 Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber Tuladhar, Slesha Clark, Scott Habib, Ahasan Materials (Basel) Article Among various available 3D bioprinting techniques, extrusion-based three-dimensional (3D) bioprinting allows the deposition of cell-laden bioink, ensuring predefined scaffold architecture that may offer living tissue regeneration. With a combination of unique characteristics such as biocompatibility, less cell toxicity, and high water content, natural hydrogels are a great candidate for bioink formulation for the extrusion-based 3D bioprinting process. However, due to its low mechanical integrity, hydrogel faces a common challenge in maintaining structural integrity. To tackle this challenge, the rheological properties, specifically the shear thinning behavior (reduction of viscosity with increasing the applied load/shear rate on hydrogels) of a set of hybrid hydrogels composed of cellulose-derived nanofiber (TEMPO-mediated nano-fibrillated cellulose, TO-NFC), carboxymethyl cellulose (CMC), and commonly used alginate, were explored. A total of 46 compositions were prepared using higher (0.5% and 1.0%) and lower percentages (0.005% and 0.01%) of TO-NFC, 1–4% of CMC, and 1–4% of alginate to analyze the shear thinning factors such as the values of n and K, which were determined for each composition from the flow diagram and co-related with the 3D printability. The ability to tune shear thinning factors with various ratios of a nanofiber can help achieve a 3D bio-printed scaffold with defined scaffold architecture. MDPI 2023-01-06 /pmc/articles/PMC9861940/ /pubmed/36676319 http://dx.doi.org/10.3390/ma16020572 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tuladhar, Slesha
Clark, Scott
Habib, Ahasan
Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber
title Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber
title_full Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber
title_fullStr Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber
title_full_unstemmed Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber
title_short Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber
title_sort tuning shear thinning factors of 3d bio-printable hydrogels using short fiber
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9861940/
https://www.ncbi.nlm.nih.gov/pubmed/36676319
http://dx.doi.org/10.3390/ma16020572
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