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Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1
Esophageal squamous cell carcinoma is a severe malignancy for its high mortality and poor prognosis. Mainstay chemotherapies cause serious side effects for their ways of inducing cell death. Oridonin is the main bioactive constituent from natural plants that has anticancer ability and weak side effe...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862004/ https://www.ncbi.nlm.nih.gov/pubmed/36677861 http://dx.doi.org/10.3390/molecules28020805 |
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author | Zhang, Xiaojun Xing, Mengtao Ma, Yangcheng Zhang, Zhuangli Qiu, Cuipeng Wang, Xiao Zhao, Zhihong Ji, Zhenyu Zhang, Jian-Ying |
author_facet | Zhang, Xiaojun Xing, Mengtao Ma, Yangcheng Zhang, Zhuangli Qiu, Cuipeng Wang, Xiao Zhao, Zhihong Ji, Zhenyu Zhang, Jian-Ying |
author_sort | Zhang, Xiaojun |
collection | PubMed |
description | Esophageal squamous cell carcinoma is a severe malignancy for its high mortality and poor prognosis. Mainstay chemotherapies cause serious side effects for their ways of inducing cell death. Oridonin is the main bioactive constituent from natural plants that has anticancer ability and weak side effects. The proteomics method is efficient to understand the anticancer mechanism. However, proteins identified by proteomics aimed at understanding oridonin’s anticancer mechanism is seldom overlapped by different groups. This study used proteomics based on two-dimensional electrophoresis sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-DE SDS-PAGE) integrated with mass spectrometry and Gene Set Enrichment Analysis (GSEA) to understand the anticancer mechanism of oridonin on esophageal squamous cell carcinoma (ESCC). The results showed that oridonin induced ESCC cell death via apoptosis by decreasing the protein expression of LASP1 and PDLIM1. |
format | Online Article Text |
id | pubmed-9862004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98620042023-01-22 Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1 Zhang, Xiaojun Xing, Mengtao Ma, Yangcheng Zhang, Zhuangli Qiu, Cuipeng Wang, Xiao Zhao, Zhihong Ji, Zhenyu Zhang, Jian-Ying Molecules Article Esophageal squamous cell carcinoma is a severe malignancy for its high mortality and poor prognosis. Mainstay chemotherapies cause serious side effects for their ways of inducing cell death. Oridonin is the main bioactive constituent from natural plants that has anticancer ability and weak side effects. The proteomics method is efficient to understand the anticancer mechanism. However, proteins identified by proteomics aimed at understanding oridonin’s anticancer mechanism is seldom overlapped by different groups. This study used proteomics based on two-dimensional electrophoresis sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-DE SDS-PAGE) integrated with mass spectrometry and Gene Set Enrichment Analysis (GSEA) to understand the anticancer mechanism of oridonin on esophageal squamous cell carcinoma (ESCC). The results showed that oridonin induced ESCC cell death via apoptosis by decreasing the protein expression of LASP1 and PDLIM1. MDPI 2023-01-13 /pmc/articles/PMC9862004/ /pubmed/36677861 http://dx.doi.org/10.3390/molecules28020805 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Xiaojun Xing, Mengtao Ma, Yangcheng Zhang, Zhuangli Qiu, Cuipeng Wang, Xiao Zhao, Zhihong Ji, Zhenyu Zhang, Jian-Ying Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1 |
title | Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1 |
title_full | Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1 |
title_fullStr | Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1 |
title_full_unstemmed | Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1 |
title_short | Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1 |
title_sort | oridonin induces apoptosis in esophageal squamous cell carcinoma by inhibiting cytoskeletal protein lasp1 and pdlim1 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862004/ https://www.ncbi.nlm.nih.gov/pubmed/36677861 http://dx.doi.org/10.3390/molecules28020805 |
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