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Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer

Both genetic improvement and the application of N fertilizer increase the quality and yields of wheat. However, the molecular kinetics that underlies the differences between them are not well understood. In this study, we performed a non-targeted metabolomic analysis on wheat cultivars from differen...

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Autores principales: Zhao, Fulin, Wang, Yifan, Hu, Jiayu, Shi, Shaolei, Zhang, Hongyan, Wang, Yang, Ye, Youliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862063/
https://www.ncbi.nlm.nih.gov/pubmed/36677032
http://dx.doi.org/10.3390/metabo13010107
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author Zhao, Fulin
Wang, Yifan
Hu, Jiayu
Shi, Shaolei
Zhang, Hongyan
Wang, Yang
Ye, Youliang
author_facet Zhao, Fulin
Wang, Yifan
Hu, Jiayu
Shi, Shaolei
Zhang, Hongyan
Wang, Yang
Ye, Youliang
author_sort Zhao, Fulin
collection PubMed
description Both genetic improvement and the application of N fertilizer increase the quality and yields of wheat. However, the molecular kinetics that underlies the differences between them are not well understood. In this study, we performed a non-targeted metabolomic analysis on wheat cultivars from different release years to comprehensively investigate the metabolic differences between cultivar and N treatments. The results revealed that the plant height and tiller number steadily decreased with increased ears numbers, whereas the grain number and weight increased with genetic improvement. Following the addition of N fertilizer, the panicle numbers and grain weights increased in an old cultivar, whereas the panicle number and grain number per panicle increased in a modern cultivar. For the 1950s to 2010s cultivar, the yield increases due to genetic improvements ranged from −1.9% to 96.7%, whereas that of N application ranged from 19.1% to 81.6%. Based on the untargeted metabolomics approach, the findings demonstrated that genetic improvements induced 1.4 to 7.4 times more metabolic alterations than N fertilizer supply. After the addition of N, 69.6%, 29.4%, and 33.3% of the differential metabolites were upregulated in the 1950s, 1980s, and 2010s cultivars, respectively. The results of metabolic pathway analysis of the identified differential metabolites via genetic improvement indicated enrichment in 1-2 KEGG pathways, whereas the application of N fertilizer enriched 2–4 pathways. Our results provide new insights into the molecular mechanisms of wheat quality and grain yield developments.
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spelling pubmed-98620632023-01-22 Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer Zhao, Fulin Wang, Yifan Hu, Jiayu Shi, Shaolei Zhang, Hongyan Wang, Yang Ye, Youliang Metabolites Article Both genetic improvement and the application of N fertilizer increase the quality and yields of wheat. However, the molecular kinetics that underlies the differences between them are not well understood. In this study, we performed a non-targeted metabolomic analysis on wheat cultivars from different release years to comprehensively investigate the metabolic differences between cultivar and N treatments. The results revealed that the plant height and tiller number steadily decreased with increased ears numbers, whereas the grain number and weight increased with genetic improvement. Following the addition of N fertilizer, the panicle numbers and grain weights increased in an old cultivar, whereas the panicle number and grain number per panicle increased in a modern cultivar. For the 1950s to 2010s cultivar, the yield increases due to genetic improvements ranged from −1.9% to 96.7%, whereas that of N application ranged from 19.1% to 81.6%. Based on the untargeted metabolomics approach, the findings demonstrated that genetic improvements induced 1.4 to 7.4 times more metabolic alterations than N fertilizer supply. After the addition of N, 69.6%, 29.4%, and 33.3% of the differential metabolites were upregulated in the 1950s, 1980s, and 2010s cultivars, respectively. The results of metabolic pathway analysis of the identified differential metabolites via genetic improvement indicated enrichment in 1-2 KEGG pathways, whereas the application of N fertilizer enriched 2–4 pathways. Our results provide new insights into the molecular mechanisms of wheat quality and grain yield developments. MDPI 2023-01-09 /pmc/articles/PMC9862063/ /pubmed/36677032 http://dx.doi.org/10.3390/metabo13010107 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Fulin
Wang, Yifan
Hu, Jiayu
Shi, Shaolei
Zhang, Hongyan
Wang, Yang
Ye, Youliang
Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer
title Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer
title_full Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer
title_fullStr Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer
title_full_unstemmed Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer
title_short Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer
title_sort metabolite profiling of wheat response to cultivar improvement and nitrogen fertilizer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862063/
https://www.ncbi.nlm.nih.gov/pubmed/36677032
http://dx.doi.org/10.3390/metabo13010107
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