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Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen

Biological control of postharvest diseases has been proven to be an effective alternative to chemical control. As an environmentally friendly biocontrol agent, Bacillus subtilis has been widely applied. This study explores its application in kiwifruit soft rot and reveals the corresponding mechanism...

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Autores principales: Fan, Yezhen, Liu, Kui, Lu, Ruoxi, Gao, Jieyu, Song, Wu, Zhu, Hongyan, Tang, Xiaofeng, Liu, Yongsheng, Miao, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862322/
https://www.ncbi.nlm.nih.gov/pubmed/36675948
http://dx.doi.org/10.3390/jof9010127
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author Fan, Yezhen
Liu, Kui
Lu, Ruoxi
Gao, Jieyu
Song, Wu
Zhu, Hongyan
Tang, Xiaofeng
Liu, Yongsheng
Miao, Min
author_facet Fan, Yezhen
Liu, Kui
Lu, Ruoxi
Gao, Jieyu
Song, Wu
Zhu, Hongyan
Tang, Xiaofeng
Liu, Yongsheng
Miao, Min
author_sort Fan, Yezhen
collection PubMed
description Biological control of postharvest diseases has been proven to be an effective alternative to chemical control. As an environmentally friendly biocontrol agent, Bacillus subtilis has been widely applied. This study explores its application in kiwifruit soft rot and reveals the corresponding mechanisms. Treatment with cell-free supernatant (CFS) of Bacillus subtilis BS-1 significantly inhibits the mycelial growth of the pathogen Botryosphaeria dothidea and attenuates the pathogenicity on kiwifruit in a concentration-dependent manner. In particular, mycelial growth diameter was only 21% of the control after 3 days of treatment with 5% CFS. CFS caused swelling and breakage of the hyphae of B. dothidea observed by scanning electron microscopy, resulting in the leakage of nucleic acid and soluble protein and the loss of ergosterol content. Further analysis demonstrated that CFS significantly induces the expression of Nox genes associated with reactive oxygen species (ROS) production by 1.9–2.7-fold, leading to a considerable accumulation of ROS in cells and causing mycelial cell death. Our findings demonstrate that the biocontrol effect of B. subtilis BS-1 CFS on B. dothidea is realized by inducing oxidative damage to the mycelia cell.
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spelling pubmed-98623222023-01-22 Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen Fan, Yezhen Liu, Kui Lu, Ruoxi Gao, Jieyu Song, Wu Zhu, Hongyan Tang, Xiaofeng Liu, Yongsheng Miao, Min J Fungi (Basel) Article Biological control of postharvest diseases has been proven to be an effective alternative to chemical control. As an environmentally friendly biocontrol agent, Bacillus subtilis has been widely applied. This study explores its application in kiwifruit soft rot and reveals the corresponding mechanisms. Treatment with cell-free supernatant (CFS) of Bacillus subtilis BS-1 significantly inhibits the mycelial growth of the pathogen Botryosphaeria dothidea and attenuates the pathogenicity on kiwifruit in a concentration-dependent manner. In particular, mycelial growth diameter was only 21% of the control after 3 days of treatment with 5% CFS. CFS caused swelling and breakage of the hyphae of B. dothidea observed by scanning electron microscopy, resulting in the leakage of nucleic acid and soluble protein and the loss of ergosterol content. Further analysis demonstrated that CFS significantly induces the expression of Nox genes associated with reactive oxygen species (ROS) production by 1.9–2.7-fold, leading to a considerable accumulation of ROS in cells and causing mycelial cell death. Our findings demonstrate that the biocontrol effect of B. subtilis BS-1 CFS on B. dothidea is realized by inducing oxidative damage to the mycelia cell. MDPI 2023-01-16 /pmc/articles/PMC9862322/ /pubmed/36675948 http://dx.doi.org/10.3390/jof9010127 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fan, Yezhen
Liu, Kui
Lu, Ruoxi
Gao, Jieyu
Song, Wu
Zhu, Hongyan
Tang, Xiaofeng
Liu, Yongsheng
Miao, Min
Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen
title Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen
title_full Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen
title_fullStr Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen
title_full_unstemmed Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen
title_short Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen
title_sort cell-free supernatant of bacillus subtilis reduces kiwifruit rot caused by botryosphaeria dothidea through inducing oxidative stress in the pathogen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862322/
https://www.ncbi.nlm.nih.gov/pubmed/36675948
http://dx.doi.org/10.3390/jof9010127
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