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Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism

Myopathies have risen strongly in recent years, likely linked to selection for appetite. For white striping (WS), causes have been identified; but for wooden breast (WB), the cause remains speculative. We used metabolomics to study the breast muscle of 51 birds that were scored for both at 35 days o...

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Autores principales: Boerboom, Gavin M., Navarro-Villa, Alberto, van Kempen, Theo A. T. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862534/
https://www.ncbi.nlm.nih.gov/pubmed/36676945
http://dx.doi.org/10.3390/metabo13010020
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author Boerboom, Gavin M.
Navarro-Villa, Alberto
van Kempen, Theo A. T. G.
author_facet Boerboom, Gavin M.
Navarro-Villa, Alberto
van Kempen, Theo A. T. G.
author_sort Boerboom, Gavin M.
collection PubMed
description Myopathies have risen strongly in recent years, likely linked to selection for appetite. For white striping (WS), causes have been identified; but for wooden breast (WB), the cause remains speculative. We used metabolomics to study the breast muscle of 51 birds that were scored for both at 35 days of age to better understand potential causes. A partial least square discriminant analysis revealed that WS and WB had distinct metabolic profiles, implying different etiologies. Arginine and proline metabolism were affected in both, although differently: WB increased arginine in breast muscle implying that the birds did not use this pathway to increase tissue blood flow. Antioxidant defenses were impeded as shown by low anserine and beta-alanine. In contrast, GSH and selenium concentrations were increased. Serine, linked to anti-inflammatory properties, was increased. Taurine, which can stabilize the cell’s sarcolemma as well as modulate potassium channels and cellular calcium homeostasis, was also increased. Mineral data and depressed phosphatidylethanolamine, cAMP, and creatine-phosphate suggested compromised energy metabolism. WB also had drastically lower diet-derived lipids, suggesting compromised lipid digestion. In conclusion, WB may be caused by impaired lipid digestion triggered by a very high appetite: the ensuing deficiencies may well impair blood flow into muscle resulting in irreparable damage.
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spelling pubmed-98625342023-01-22 Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism Boerboom, Gavin M. Navarro-Villa, Alberto van Kempen, Theo A. T. G. Metabolites Article Myopathies have risen strongly in recent years, likely linked to selection for appetite. For white striping (WS), causes have been identified; but for wooden breast (WB), the cause remains speculative. We used metabolomics to study the breast muscle of 51 birds that were scored for both at 35 days of age to better understand potential causes. A partial least square discriminant analysis revealed that WS and WB had distinct metabolic profiles, implying different etiologies. Arginine and proline metabolism were affected in both, although differently: WB increased arginine in breast muscle implying that the birds did not use this pathway to increase tissue blood flow. Antioxidant defenses were impeded as shown by low anserine and beta-alanine. In contrast, GSH and selenium concentrations were increased. Serine, linked to anti-inflammatory properties, was increased. Taurine, which can stabilize the cell’s sarcolemma as well as modulate potassium channels and cellular calcium homeostasis, was also increased. Mineral data and depressed phosphatidylethanolamine, cAMP, and creatine-phosphate suggested compromised energy metabolism. WB also had drastically lower diet-derived lipids, suggesting compromised lipid digestion. In conclusion, WB may be caused by impaired lipid digestion triggered by a very high appetite: the ensuing deficiencies may well impair blood flow into muscle resulting in irreparable damage. MDPI 2022-12-22 /pmc/articles/PMC9862534/ /pubmed/36676945 http://dx.doi.org/10.3390/metabo13010020 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boerboom, Gavin M.
Navarro-Villa, Alberto
van Kempen, Theo A. T. G.
Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism
title Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism
title_full Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism
title_fullStr Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism
title_full_unstemmed Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism
title_short Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism
title_sort metabolomic analysis of wooden breast myopathy shows a disturbed lipid metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862534/
https://www.ncbi.nlm.nih.gov/pubmed/36676945
http://dx.doi.org/10.3390/metabo13010020
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