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Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech

Sponges are the richest source of bioactive organic small molecules, referred to as natural products, in the marine environment. It is well established that laboratory culturing-resistant symbiotic bacteria residing within the eukaryotic sponge host matrix often synthesize the natural products that...

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Autores principales: Deutsch, Jessica M., Green, Madison O., Akavaram, Priyanka, Davis, Ashleigh C., Diskalkar, Sarth S., Du Plessis, Isabelle A., Fallon, Hannah A., Grason, Emma M., Kauf, Emma G., Kim, Zoe M., Miller, Jeffrey R., Neal, Abby L., Riera, Tatiana, Stroeva, Sofie-Ellen, Tran, Jollin, Tran, Vivi, Coronado, Azucena Velgara, Coronado, Vanessa Velgara, Wall, Benjamin T., Yang, Chung mo, Mohanty, Ipsita, Abrahamse, Nadine H., Freeman, Christopher J., Easson, Cole G., Fiore, Cara L., Onstine, Alison E., Djeddar, Naima, Biliya, Shweta, Bryksin, Anton V., Garg, Neha, Agarwal, Vinayak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862627/
https://www.ncbi.nlm.nih.gov/pubmed/36662226
http://dx.doi.org/10.3390/md21010053
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author Deutsch, Jessica M.
Green, Madison O.
Akavaram, Priyanka
Davis, Ashleigh C.
Diskalkar, Sarth S.
Du Plessis, Isabelle A.
Fallon, Hannah A.
Grason, Emma M.
Kauf, Emma G.
Kim, Zoe M.
Miller, Jeffrey R.
Neal, Abby L.
Riera, Tatiana
Stroeva, Sofie-Ellen
Tran, Jollin
Tran, Vivi
Coronado, Azucena Velgara
Coronado, Vanessa Velgara
Wall, Benjamin T.
Yang, Chung mo
Mohanty, Ipsita
Abrahamse, Nadine H.
Freeman, Christopher J.
Easson, Cole G.
Fiore, Cara L.
Onstine, Alison E.
Djeddar, Naima
Biliya, Shweta
Bryksin, Anton V.
Garg, Neha
Agarwal, Vinayak
author_facet Deutsch, Jessica M.
Green, Madison O.
Akavaram, Priyanka
Davis, Ashleigh C.
Diskalkar, Sarth S.
Du Plessis, Isabelle A.
Fallon, Hannah A.
Grason, Emma M.
Kauf, Emma G.
Kim, Zoe M.
Miller, Jeffrey R.
Neal, Abby L.
Riera, Tatiana
Stroeva, Sofie-Ellen
Tran, Jollin
Tran, Vivi
Coronado, Azucena Velgara
Coronado, Vanessa Velgara
Wall, Benjamin T.
Yang, Chung mo
Mohanty, Ipsita
Abrahamse, Nadine H.
Freeman, Christopher J.
Easson, Cole G.
Fiore, Cara L.
Onstine, Alison E.
Djeddar, Naima
Biliya, Shweta
Bryksin, Anton V.
Garg, Neha
Agarwal, Vinayak
author_sort Deutsch, Jessica M.
collection PubMed
description Sponges are the richest source of bioactive organic small molecules, referred to as natural products, in the marine environment. It is well established that laboratory culturing-resistant symbiotic bacteria residing within the eukaryotic sponge host matrix often synthesize the natural products that are detected in the sponge tissue extracts. However, the contributions of the culturing-amenable commensal bacteria that are also associated with the sponge host to the overall metabolome of the sponge holobiont are not well defined. In this study, we cultured a large library of bacteria from three marine sponges commonly found in the Florida Keys. Metabolomes of isolated bacterial strains and that of the sponge holobiont were compared using mass spectrometry to reveal minimal metabolomic overlap between commensal bacteria and the sponge hosts. We also find that the phylogenetic overlap between cultured commensal bacteria and that of the sponge microbiome is minimal. Despite these observations, the commensal bacteria were found to be a rich resource for novel natural product discovery. Mass spectrometry-based metabolomics provided structural insights into these cryptic natural products. Pedagogic innovation in the form of laboratory curricula development is described which provided undergraduate students with hands-on instruction in microbiology and natural product discovery using metabolomic data mining strategies.
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spelling pubmed-98626272023-01-22 Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech Deutsch, Jessica M. Green, Madison O. Akavaram, Priyanka Davis, Ashleigh C. Diskalkar, Sarth S. Du Plessis, Isabelle A. Fallon, Hannah A. Grason, Emma M. Kauf, Emma G. Kim, Zoe M. Miller, Jeffrey R. Neal, Abby L. Riera, Tatiana Stroeva, Sofie-Ellen Tran, Jollin Tran, Vivi Coronado, Azucena Velgara Coronado, Vanessa Velgara Wall, Benjamin T. Yang, Chung mo Mohanty, Ipsita Abrahamse, Nadine H. Freeman, Christopher J. Easson, Cole G. Fiore, Cara L. Onstine, Alison E. Djeddar, Naima Biliya, Shweta Bryksin, Anton V. Garg, Neha Agarwal, Vinayak Mar Drugs Article Sponges are the richest source of bioactive organic small molecules, referred to as natural products, in the marine environment. It is well established that laboratory culturing-resistant symbiotic bacteria residing within the eukaryotic sponge host matrix often synthesize the natural products that are detected in the sponge tissue extracts. However, the contributions of the culturing-amenable commensal bacteria that are also associated with the sponge host to the overall metabolome of the sponge holobiont are not well defined. In this study, we cultured a large library of bacteria from three marine sponges commonly found in the Florida Keys. Metabolomes of isolated bacterial strains and that of the sponge holobiont were compared using mass spectrometry to reveal minimal metabolomic overlap between commensal bacteria and the sponge hosts. We also find that the phylogenetic overlap between cultured commensal bacteria and that of the sponge microbiome is minimal. Despite these observations, the commensal bacteria were found to be a rich resource for novel natural product discovery. Mass spectrometry-based metabolomics provided structural insights into these cryptic natural products. Pedagogic innovation in the form of laboratory curricula development is described which provided undergraduate students with hands-on instruction in microbiology and natural product discovery using metabolomic data mining strategies. MDPI 2023-01-14 /pmc/articles/PMC9862627/ /pubmed/36662226 http://dx.doi.org/10.3390/md21010053 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Deutsch, Jessica M.
Green, Madison O.
Akavaram, Priyanka
Davis, Ashleigh C.
Diskalkar, Sarth S.
Du Plessis, Isabelle A.
Fallon, Hannah A.
Grason, Emma M.
Kauf, Emma G.
Kim, Zoe M.
Miller, Jeffrey R.
Neal, Abby L.
Riera, Tatiana
Stroeva, Sofie-Ellen
Tran, Jollin
Tran, Vivi
Coronado, Azucena Velgara
Coronado, Vanessa Velgara
Wall, Benjamin T.
Yang, Chung mo
Mohanty, Ipsita
Abrahamse, Nadine H.
Freeman, Christopher J.
Easson, Cole G.
Fiore, Cara L.
Onstine, Alison E.
Djeddar, Naima
Biliya, Shweta
Bryksin, Anton V.
Garg, Neha
Agarwal, Vinayak
Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech
title Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech
title_full Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech
title_fullStr Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech
title_full_unstemmed Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech
title_short Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech
title_sort limited metabolomic overlap between commensal bacteria and marine sponge holobionts revealed by large scale culturing and mass spectrometry-based metabolomics: an undergraduate laboratory pedagogical effort at georgia tech
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862627/
https://www.ncbi.nlm.nih.gov/pubmed/36662226
http://dx.doi.org/10.3390/md21010053
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