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Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study
Periodontal regeneration through the employment of bone substitutes has become a feasible strategy in animal and clinical studies. In this regard, we aimed to compare the periodontal ligament stem cell behavior in the vicinity of various bone grafting substitutes. Three types of popular bone substit...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862872/ https://www.ncbi.nlm.nih.gov/pubmed/36676038 http://dx.doi.org/10.3390/life13010089 |
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author | Esfahanian, Vahid Ejeian, Fatemeh Mohebinia, Hajar Zojaji Nejad, Zahra Sadat Yazdchi, Maryam Ebrahimi Dastgerdi, Maziar Ebrahimi Dastgerdi, Mehrnoush Nasr-Esfahani, Mohammad Hossein |
author_facet | Esfahanian, Vahid Ejeian, Fatemeh Mohebinia, Hajar Zojaji Nejad, Zahra Sadat Yazdchi, Maryam Ebrahimi Dastgerdi, Maziar Ebrahimi Dastgerdi, Mehrnoush Nasr-Esfahani, Mohammad Hossein |
author_sort | Esfahanian, Vahid |
collection | PubMed |
description | Periodontal regeneration through the employment of bone substitutes has become a feasible strategy in animal and clinical studies. In this regard, we aimed to compare the periodontal ligament stem cell behavior in the vicinity of various bone grafting substitutes. Three types of popular bone substitutes, including allografts (Regen), xenografts (Cerabone), and alloplasts (Osteon) were studied in this experimental survey. The cellular attachment was assessed after four hours using the MTS assay and SEM imaging. In addition, cellular proliferation was investigated after 1, 3, 5, and 7 days through MTS assay. Osteogenesis was studied after 21 days of cell culture in a differentiation medium (DM+) and a normal medium (DM−), by employing real-time PCR and alizarin red staining. The highest cellular attachment was seen in the xenograft group with a significant difference in comparison to the other grafting materials. Despite the relatively low primary attachment of cells to allografts, the allograft group showed the highest total proliferation rate, while the lowest proliferation capacity was found in the alloplast group. Osteogenesis fount to be accelerated mostly by xenografts in both mediums (DM+ and DM−) after 3 weeks, while alloplasts showed the lowest osteogenesis. This study revealed that the type of bone substitutes used in regenerative treatments can affect cellular behavior and as a whole allografts and xenografts showed better results. |
format | Online Article Text |
id | pubmed-9862872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98628722023-01-22 Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study Esfahanian, Vahid Ejeian, Fatemeh Mohebinia, Hajar Zojaji Nejad, Zahra Sadat Yazdchi, Maryam Ebrahimi Dastgerdi, Maziar Ebrahimi Dastgerdi, Mehrnoush Nasr-Esfahani, Mohammad Hossein Life (Basel) Article Periodontal regeneration through the employment of bone substitutes has become a feasible strategy in animal and clinical studies. In this regard, we aimed to compare the periodontal ligament stem cell behavior in the vicinity of various bone grafting substitutes. Three types of popular bone substitutes, including allografts (Regen), xenografts (Cerabone), and alloplasts (Osteon) were studied in this experimental survey. The cellular attachment was assessed after four hours using the MTS assay and SEM imaging. In addition, cellular proliferation was investigated after 1, 3, 5, and 7 days through MTS assay. Osteogenesis was studied after 21 days of cell culture in a differentiation medium (DM+) and a normal medium (DM−), by employing real-time PCR and alizarin red staining. The highest cellular attachment was seen in the xenograft group with a significant difference in comparison to the other grafting materials. Despite the relatively low primary attachment of cells to allografts, the allograft group showed the highest total proliferation rate, while the lowest proliferation capacity was found in the alloplast group. Osteogenesis fount to be accelerated mostly by xenografts in both mediums (DM+ and DM−) after 3 weeks, while alloplasts showed the lowest osteogenesis. This study revealed that the type of bone substitutes used in regenerative treatments can affect cellular behavior and as a whole allografts and xenografts showed better results. MDPI 2022-12-28 /pmc/articles/PMC9862872/ /pubmed/36676038 http://dx.doi.org/10.3390/life13010089 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Esfahanian, Vahid Ejeian, Fatemeh Mohebinia, Hajar Zojaji Nejad, Zahra Sadat Yazdchi, Maryam Ebrahimi Dastgerdi, Maziar Ebrahimi Dastgerdi, Mehrnoush Nasr-Esfahani, Mohammad Hossein Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study |
title | Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study |
title_full | Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study |
title_fullStr | Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study |
title_full_unstemmed | Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study |
title_short | Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study |
title_sort | cellular behaviors of periodontal ligament stem cells in the presence of bone grafting biomaterials, in-vitro study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9862872/ https://www.ncbi.nlm.nih.gov/pubmed/36676038 http://dx.doi.org/10.3390/life13010089 |
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