Cargando…
The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids
Shaziling pigs, as a native Chinese breed, have been classified as a fatty liver model. As the core of the whole pig farm, the sow’s organism health is especially important, especially in the perinatal period; however, there are few reports on the perinatal intestinal microbiology and bile acid meta...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863110/ https://www.ncbi.nlm.nih.gov/pubmed/36676993 http://dx.doi.org/10.3390/metabo13010068 |
_version_ | 1784875254686941184 |
---|---|
author | Wang, Jie Li, Yulian Cao, Chang Yang, Runhua He, Meilin Yan, Jiaqi Huang, Peng Tan, Bie Fan, Zhiyong |
author_facet | Wang, Jie Li, Yulian Cao, Chang Yang, Runhua He, Meilin Yan, Jiaqi Huang, Peng Tan, Bie Fan, Zhiyong |
author_sort | Wang, Jie |
collection | PubMed |
description | Shaziling pigs, as a native Chinese breed, have been classified as a fatty liver model. As the core of the whole pig farm, the sow’s organism health is especially important, especially in the perinatal period; however, there are few reports on the perinatal intestinal microbiology and bile acid metabolism of Shaziling pig sows. The purpose of this research was to investigate the alterations in bile acids and gut microbiota of sows that occur throughout the perinatal period. Forty-two sows were selected for their uniformity of body conditions and were given the same diet. Fecal samples were collected for 16srDNA sequencing and bile acid targeted metabolome detection in four stages (3 days before delivery, 3 days after delivery, 7 days after delivery and 21 days after delivery). As revealed by the results, there were statistically significant variations in bile acids among the four stages, with the concentration of bile acids identified by SZL-4 being substantially greater than that of the other three groups (p < 0.05). When compared to the other three groups (p < 0.05), SZL-2 had considerably lower Shannon, Simpson and Chao 1 indices, and exhibited a statistically significant difference in β-diversity. SZL-2 samples included a greater proportion of Proteobacteria than SZL-3 and SZL-4 samples; however, SZL-2 samples contained a smaller proportion of spirochetes than SZL-3 and SZL-4 samples. To a large extent, lactic acid bacteria predominated in the SZL-2 samples. The LEfSe analysis showed that the relative abundances of Lachnospiraceae_XPB1014_group, Christensenellaceae_R_7_group, Clostridium, Collinsella, Turicibacter, and Mollicutes_RF39_unclassified were the main differential bacteria in the SZL-1 swine fecal samples and the Eubacterium__coprostanoligenes_group in sow fecal samples from SZL-2. The relative abundance of Bacteroides, UBA1819, Enterococcus, Erysipelatoclostridium, and Butyricimonas in SZL-3 and SZL-4 Streptococcus, Coriobacteriaceae_unclassified, Prevotellaceae_UCG_001, Streptomyces, and Ochrobactrum in SZL-3. g_Collinsella was significantly and positively correlated with vast majority bile acids, and the g_Lachnospiraceae_XPB1014_group with GCDCA and GHDCA into positive correlations. Simultaneously, g_Streptococcus, g_Bacteroides, and g_UBA1819 inversely correlated with bile acid, accounting for the great bulk of the difference. In conclusion, there is an evident correlation between bile acids and gut microbiota in the perinatal period of Shaziling sows. Additionally, the discovery of distinct bacteria associated to lipid metabolism gives a reference for ameliorating perinatal body lipid metabolism disorder of sows through gut microbiota. |
format | Online Article Text |
id | pubmed-9863110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98631102023-01-22 The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids Wang, Jie Li, Yulian Cao, Chang Yang, Runhua He, Meilin Yan, Jiaqi Huang, Peng Tan, Bie Fan, Zhiyong Metabolites Article Shaziling pigs, as a native Chinese breed, have been classified as a fatty liver model. As the core of the whole pig farm, the sow’s organism health is especially important, especially in the perinatal period; however, there are few reports on the perinatal intestinal microbiology and bile acid metabolism of Shaziling pig sows. The purpose of this research was to investigate the alterations in bile acids and gut microbiota of sows that occur throughout the perinatal period. Forty-two sows were selected for their uniformity of body conditions and were given the same diet. Fecal samples were collected for 16srDNA sequencing and bile acid targeted metabolome detection in four stages (3 days before delivery, 3 days after delivery, 7 days after delivery and 21 days after delivery). As revealed by the results, there were statistically significant variations in bile acids among the four stages, with the concentration of bile acids identified by SZL-4 being substantially greater than that of the other three groups (p < 0.05). When compared to the other three groups (p < 0.05), SZL-2 had considerably lower Shannon, Simpson and Chao 1 indices, and exhibited a statistically significant difference in β-diversity. SZL-2 samples included a greater proportion of Proteobacteria than SZL-3 and SZL-4 samples; however, SZL-2 samples contained a smaller proportion of spirochetes than SZL-3 and SZL-4 samples. To a large extent, lactic acid bacteria predominated in the SZL-2 samples. The LEfSe analysis showed that the relative abundances of Lachnospiraceae_XPB1014_group, Christensenellaceae_R_7_group, Clostridium, Collinsella, Turicibacter, and Mollicutes_RF39_unclassified were the main differential bacteria in the SZL-1 swine fecal samples and the Eubacterium__coprostanoligenes_group in sow fecal samples from SZL-2. The relative abundance of Bacteroides, UBA1819, Enterococcus, Erysipelatoclostridium, and Butyricimonas in SZL-3 and SZL-4 Streptococcus, Coriobacteriaceae_unclassified, Prevotellaceae_UCG_001, Streptomyces, and Ochrobactrum in SZL-3. g_Collinsella was significantly and positively correlated with vast majority bile acids, and the g_Lachnospiraceae_XPB1014_group with GCDCA and GHDCA into positive correlations. Simultaneously, g_Streptococcus, g_Bacteroides, and g_UBA1819 inversely correlated with bile acid, accounting for the great bulk of the difference. In conclusion, there is an evident correlation between bile acids and gut microbiota in the perinatal period of Shaziling sows. Additionally, the discovery of distinct bacteria associated to lipid metabolism gives a reference for ameliorating perinatal body lipid metabolism disorder of sows through gut microbiota. MDPI 2023-01-01 /pmc/articles/PMC9863110/ /pubmed/36676993 http://dx.doi.org/10.3390/metabo13010068 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Jie Li, Yulian Cao, Chang Yang, Runhua He, Meilin Yan, Jiaqi Huang, Peng Tan, Bie Fan, Zhiyong The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids |
title | The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids |
title_full | The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids |
title_fullStr | The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids |
title_full_unstemmed | The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids |
title_short | The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids |
title_sort | periparturient gut microbiota’s modifications in shaziling sows concerning bile acids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863110/ https://www.ncbi.nlm.nih.gov/pubmed/36676993 http://dx.doi.org/10.3390/metabo13010068 |
work_keys_str_mv | AT wangjie theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT liyulian theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT caochang theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT yangrunhua theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT hemeilin theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT yanjiaqi theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT huangpeng theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT tanbie theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT fanzhiyong theperiparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT wangjie periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT liyulian periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT caochang periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT yangrunhua periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT hemeilin periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT yanjiaqi periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT huangpeng periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT tanbie periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids AT fanzhiyong periparturientgutmicrobiotasmodificationsinshazilingsowsconcerningbileacids |