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The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids

Shaziling pigs, as a native Chinese breed, have been classified as a fatty liver model. As the core of the whole pig farm, the sow’s organism health is especially important, especially in the perinatal period; however, there are few reports on the perinatal intestinal microbiology and bile acid meta...

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Autores principales: Wang, Jie, Li, Yulian, Cao, Chang, Yang, Runhua, He, Meilin, Yan, Jiaqi, Huang, Peng, Tan, Bie, Fan, Zhiyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863110/
https://www.ncbi.nlm.nih.gov/pubmed/36676993
http://dx.doi.org/10.3390/metabo13010068
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author Wang, Jie
Li, Yulian
Cao, Chang
Yang, Runhua
He, Meilin
Yan, Jiaqi
Huang, Peng
Tan, Bie
Fan, Zhiyong
author_facet Wang, Jie
Li, Yulian
Cao, Chang
Yang, Runhua
He, Meilin
Yan, Jiaqi
Huang, Peng
Tan, Bie
Fan, Zhiyong
author_sort Wang, Jie
collection PubMed
description Shaziling pigs, as a native Chinese breed, have been classified as a fatty liver model. As the core of the whole pig farm, the sow’s organism health is especially important, especially in the perinatal period; however, there are few reports on the perinatal intestinal microbiology and bile acid metabolism of Shaziling pig sows. The purpose of this research was to investigate the alterations in bile acids and gut microbiota of sows that occur throughout the perinatal period. Forty-two sows were selected for their uniformity of body conditions and were given the same diet. Fecal samples were collected for 16srDNA sequencing and bile acid targeted metabolome detection in four stages (3 days before delivery, 3 days after delivery, 7 days after delivery and 21 days after delivery). As revealed by the results, there were statistically significant variations in bile acids among the four stages, with the concentration of bile acids identified by SZL-4 being substantially greater than that of the other three groups (p < 0.05). When compared to the other three groups (p < 0.05), SZL-2 had considerably lower Shannon, Simpson and Chao 1 indices, and exhibited a statistically significant difference in β-diversity. SZL-2 samples included a greater proportion of Proteobacteria than SZL-3 and SZL-4 samples; however, SZL-2 samples contained a smaller proportion of spirochetes than SZL-3 and SZL-4 samples. To a large extent, lactic acid bacteria predominated in the SZL-2 samples. The LEfSe analysis showed that the relative abundances of Lachnospiraceae_XPB1014_group, Christensenellaceae_R_7_group, Clostridium, Collinsella, Turicibacter, and Mollicutes_RF39_unclassified were the main differential bacteria in the SZL-1 swine fecal samples and the Eubacterium__coprostanoligenes_group in sow fecal samples from SZL-2. The relative abundance of Bacteroides, UBA1819, Enterococcus, Erysipelatoclostridium, and Butyricimonas in SZL-3 and SZL-4 Streptococcus, Coriobacteriaceae_unclassified, Prevotellaceae_UCG_001, Streptomyces, and Ochrobactrum in SZL-3. g_Collinsella was significantly and positively correlated with vast majority bile acids, and the g_Lachnospiraceae_XPB1014_group with GCDCA and GHDCA into positive correlations. Simultaneously, g_Streptococcus, g_Bacteroides, and g_UBA1819 inversely correlated with bile acid, accounting for the great bulk of the difference. In conclusion, there is an evident correlation between bile acids and gut microbiota in the perinatal period of Shaziling sows. Additionally, the discovery of distinct bacteria associated to lipid metabolism gives a reference for ameliorating perinatal body lipid metabolism disorder of sows through gut microbiota.
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spelling pubmed-98631102023-01-22 The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids Wang, Jie Li, Yulian Cao, Chang Yang, Runhua He, Meilin Yan, Jiaqi Huang, Peng Tan, Bie Fan, Zhiyong Metabolites Article Shaziling pigs, as a native Chinese breed, have been classified as a fatty liver model. As the core of the whole pig farm, the sow’s organism health is especially important, especially in the perinatal period; however, there are few reports on the perinatal intestinal microbiology and bile acid metabolism of Shaziling pig sows. The purpose of this research was to investigate the alterations in bile acids and gut microbiota of sows that occur throughout the perinatal period. Forty-two sows were selected for their uniformity of body conditions and were given the same diet. Fecal samples were collected for 16srDNA sequencing and bile acid targeted metabolome detection in four stages (3 days before delivery, 3 days after delivery, 7 days after delivery and 21 days after delivery). As revealed by the results, there were statistically significant variations in bile acids among the four stages, with the concentration of bile acids identified by SZL-4 being substantially greater than that of the other three groups (p < 0.05). When compared to the other three groups (p < 0.05), SZL-2 had considerably lower Shannon, Simpson and Chao 1 indices, and exhibited a statistically significant difference in β-diversity. SZL-2 samples included a greater proportion of Proteobacteria than SZL-3 and SZL-4 samples; however, SZL-2 samples contained a smaller proportion of spirochetes than SZL-3 and SZL-4 samples. To a large extent, lactic acid bacteria predominated in the SZL-2 samples. The LEfSe analysis showed that the relative abundances of Lachnospiraceae_XPB1014_group, Christensenellaceae_R_7_group, Clostridium, Collinsella, Turicibacter, and Mollicutes_RF39_unclassified were the main differential bacteria in the SZL-1 swine fecal samples and the Eubacterium__coprostanoligenes_group in sow fecal samples from SZL-2. The relative abundance of Bacteroides, UBA1819, Enterococcus, Erysipelatoclostridium, and Butyricimonas in SZL-3 and SZL-4 Streptococcus, Coriobacteriaceae_unclassified, Prevotellaceae_UCG_001, Streptomyces, and Ochrobactrum in SZL-3. g_Collinsella was significantly and positively correlated with vast majority bile acids, and the g_Lachnospiraceae_XPB1014_group with GCDCA and GHDCA into positive correlations. Simultaneously, g_Streptococcus, g_Bacteroides, and g_UBA1819 inversely correlated with bile acid, accounting for the great bulk of the difference. In conclusion, there is an evident correlation between bile acids and gut microbiota in the perinatal period of Shaziling sows. Additionally, the discovery of distinct bacteria associated to lipid metabolism gives a reference for ameliorating perinatal body lipid metabolism disorder of sows through gut microbiota. MDPI 2023-01-01 /pmc/articles/PMC9863110/ /pubmed/36676993 http://dx.doi.org/10.3390/metabo13010068 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jie
Li, Yulian
Cao, Chang
Yang, Runhua
He, Meilin
Yan, Jiaqi
Huang, Peng
Tan, Bie
Fan, Zhiyong
The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids
title The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids
title_full The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids
title_fullStr The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids
title_full_unstemmed The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids
title_short The Periparturient Gut Microbiota’s Modifications in Shaziling Sows concerning Bile Acids
title_sort periparturient gut microbiota’s modifications in shaziling sows concerning bile acids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863110/
https://www.ncbi.nlm.nih.gov/pubmed/36676993
http://dx.doi.org/10.3390/metabo13010068
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