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Isolation and Identification of Lipid-Lowering Peptides from Sacha Inchi Meal
Sacha inchi meal (SIM) is a by-product of sacha inchi (considered as a “super-food”) processing. In previous studies, we found that SIM protein hydrolysates exhibited pancreatic lipase inhibition activity. In this study, 10 bioactive peptides from those hydrolysates were identified. The top five pep...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863159/ https://www.ncbi.nlm.nih.gov/pubmed/36675040 http://dx.doi.org/10.3390/ijms24021529 |
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author | Wang, Kai Liu, Xiaofei Zhang, Xuewu |
author_facet | Wang, Kai Liu, Xiaofei Zhang, Xuewu |
author_sort | Wang, Kai |
collection | PubMed |
description | Sacha inchi meal (SIM) is a by-product of sacha inchi (considered as a “super-food”) processing. In previous studies, we found that SIM protein hydrolysates exhibited pancreatic lipase inhibition activity. In this study, 10 bioactive peptides from those hydrolysates were identified. The top five peptides (NLYYKVV (NV-7), WWYVK (WK-5), WLLMWPYK (WK-8), EGLLMWPY (EY-8), and FPFFGYVWK (FK-9)) with strong pancreatic lipase inhibition activity had IC(50) values of 34.01–246.50 µM, and displayed various inhibition types (mixed, non-competitive, and competitive type) by enzyme inhibition kinetics analysis. Fluorescence quenching analysis demonstrated that the interaction between the peptides and pancreatic lipase was mainly hydrogen bond and van der Waals force. The key residues involved in the peptide–enzyme interaction were determined by molecular docking. Moreover, the top two peptides were found to significantly inhibit fat accumulation and regulate lipid metabolism by alleviating the level of reactive oxygen species in HepG2 cells. Collectively, sacha inchi meal-derived peptides displayed potent lipid-lowering activity and could be used as materials of functional food. |
format | Online Article Text |
id | pubmed-9863159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98631592023-01-22 Isolation and Identification of Lipid-Lowering Peptides from Sacha Inchi Meal Wang, Kai Liu, Xiaofei Zhang, Xuewu Int J Mol Sci Article Sacha inchi meal (SIM) is a by-product of sacha inchi (considered as a “super-food”) processing. In previous studies, we found that SIM protein hydrolysates exhibited pancreatic lipase inhibition activity. In this study, 10 bioactive peptides from those hydrolysates were identified. The top five peptides (NLYYKVV (NV-7), WWYVK (WK-5), WLLMWPYK (WK-8), EGLLMWPY (EY-8), and FPFFGYVWK (FK-9)) with strong pancreatic lipase inhibition activity had IC(50) values of 34.01–246.50 µM, and displayed various inhibition types (mixed, non-competitive, and competitive type) by enzyme inhibition kinetics analysis. Fluorescence quenching analysis demonstrated that the interaction between the peptides and pancreatic lipase was mainly hydrogen bond and van der Waals force. The key residues involved in the peptide–enzyme interaction were determined by molecular docking. Moreover, the top two peptides were found to significantly inhibit fat accumulation and regulate lipid metabolism by alleviating the level of reactive oxygen species in HepG2 cells. Collectively, sacha inchi meal-derived peptides displayed potent lipid-lowering activity and could be used as materials of functional food. MDPI 2023-01-12 /pmc/articles/PMC9863159/ /pubmed/36675040 http://dx.doi.org/10.3390/ijms24021529 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Kai Liu, Xiaofei Zhang, Xuewu Isolation and Identification of Lipid-Lowering Peptides from Sacha Inchi Meal |
title | Isolation and Identification of Lipid-Lowering Peptides from Sacha Inchi Meal |
title_full | Isolation and Identification of Lipid-Lowering Peptides from Sacha Inchi Meal |
title_fullStr | Isolation and Identification of Lipid-Lowering Peptides from Sacha Inchi Meal |
title_full_unstemmed | Isolation and Identification of Lipid-Lowering Peptides from Sacha Inchi Meal |
title_short | Isolation and Identification of Lipid-Lowering Peptides from Sacha Inchi Meal |
title_sort | isolation and identification of lipid-lowering peptides from sacha inchi meal |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863159/ https://www.ncbi.nlm.nih.gov/pubmed/36675040 http://dx.doi.org/10.3390/ijms24021529 |
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