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Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse

Agro-industrial residues represent more than 60% of organic wastes worldwide, which could be used to generate other by-products or to be incorporated into other production chains. For example, bagasse is a waste from the tequila industry in Mexico that could be implemented for mushroom cultivation....

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Autores principales: Cruz-Moreno, Byanka A., Pérez, Ana Angélica Feregrino, García-Trejo, Juan Fernando, Pérez-García, Sergio Alfonso, Gutiérrez-Antonio, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863222/
https://www.ncbi.nlm.nih.gov/pubmed/36677617
http://dx.doi.org/10.3390/molecules28020557
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author Cruz-Moreno, Byanka A.
Pérez, Ana Angélica Feregrino
García-Trejo, Juan Fernando
Pérez-García, Sergio Alfonso
Gutiérrez-Antonio, Claudia
author_facet Cruz-Moreno, Byanka A.
Pérez, Ana Angélica Feregrino
García-Trejo, Juan Fernando
Pérez-García, Sergio Alfonso
Gutiérrez-Antonio, Claudia
author_sort Cruz-Moreno, Byanka A.
collection PubMed
description Agro-industrial residues represent more than 60% of organic wastes worldwide, which could be used to generate other by-products or to be incorporated into other production chains. For example, bagasse is a waste from the tequila industry in Mexico that could be implemented for mushroom cultivation. Additionally, the substrate influences the growth, development, and production of secondary metabolites of fungi. This work presents a comparative experiment that studies the metabolite production in Pleurotus djamor mushrooms on agave bagasse and barley straw (traditional substrate). The biological efficiency (BE), yield, phenolics and flavonoids, antioxidant capacity, tannins, and the identification of low molecular weight metabolites were evaluated. Five treatments were proposed according to the following mixtures of agave bagasse: barley straw: T1 (1:0), T2 (3:1), T3 (1:1), T4 (1:3), and T5 (0:1). T2 had the highest yield (13.39 ± 3.23%), BE (56.7 ± 13.71%), and flavonoids (44.25 mg rutin equivalent (RE)/g); T3 obtained the highest phenol content (230.27 mg GAE/g); and T1 the highest tannins content (0.23 mg (+) catechin equivalent (CE)/g). Finally, T1 and T5 are the ones that present the greatest number of primary metabolites, including hydroxycitric acid, 2-deoxy-D-galactose, D-mannose, paromomycin, palmitic acid, pyrrole, mannitol, and DL arabinose, while in T2, T3, and T4 only two chemical compounds were found present (palmitic acid and pyrrole in T2, silicic acid and pyrrole in T3 and 2-deoxy-D-galactose and quinoline in T4). The cultivation substrate influences the concentration of bioactive molecules in the fruiting bodies of P. djamor. Additionally, P. djamor’s degradation of agave bagasse residue generates a potential application for agro-industrial residue management at a low cost.
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spelling pubmed-98632222023-01-22 Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse Cruz-Moreno, Byanka A. Pérez, Ana Angélica Feregrino García-Trejo, Juan Fernando Pérez-García, Sergio Alfonso Gutiérrez-Antonio, Claudia Molecules Article Agro-industrial residues represent more than 60% of organic wastes worldwide, which could be used to generate other by-products or to be incorporated into other production chains. For example, bagasse is a waste from the tequila industry in Mexico that could be implemented for mushroom cultivation. Additionally, the substrate influences the growth, development, and production of secondary metabolites of fungi. This work presents a comparative experiment that studies the metabolite production in Pleurotus djamor mushrooms on agave bagasse and barley straw (traditional substrate). The biological efficiency (BE), yield, phenolics and flavonoids, antioxidant capacity, tannins, and the identification of low molecular weight metabolites were evaluated. Five treatments were proposed according to the following mixtures of agave bagasse: barley straw: T1 (1:0), T2 (3:1), T3 (1:1), T4 (1:3), and T5 (0:1). T2 had the highest yield (13.39 ± 3.23%), BE (56.7 ± 13.71%), and flavonoids (44.25 mg rutin equivalent (RE)/g); T3 obtained the highest phenol content (230.27 mg GAE/g); and T1 the highest tannins content (0.23 mg (+) catechin equivalent (CE)/g). Finally, T1 and T5 are the ones that present the greatest number of primary metabolites, including hydroxycitric acid, 2-deoxy-D-galactose, D-mannose, paromomycin, palmitic acid, pyrrole, mannitol, and DL arabinose, while in T2, T3, and T4 only two chemical compounds were found present (palmitic acid and pyrrole in T2, silicic acid and pyrrole in T3 and 2-deoxy-D-galactose and quinoline in T4). The cultivation substrate influences the concentration of bioactive molecules in the fruiting bodies of P. djamor. Additionally, P. djamor’s degradation of agave bagasse residue generates a potential application for agro-industrial residue management at a low cost. MDPI 2023-01-05 /pmc/articles/PMC9863222/ /pubmed/36677617 http://dx.doi.org/10.3390/molecules28020557 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cruz-Moreno, Byanka A.
Pérez, Ana Angélica Feregrino
García-Trejo, Juan Fernando
Pérez-García, Sergio Alfonso
Gutiérrez-Antonio, Claudia
Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse
title Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse
title_full Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse
title_fullStr Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse
title_full_unstemmed Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse
title_short Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse
title_sort identification of secondary metabolites of interest in pleurotus djamor using agave tequilana bagasse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863222/
https://www.ncbi.nlm.nih.gov/pubmed/36677617
http://dx.doi.org/10.3390/molecules28020557
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