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The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene

Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara...

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Autores principales: Li, Charng-Pei, Wu, Dong-Hong, Huang, Shou-Horng, Meng, Menghsiao, Shih, Hsien-Tzung, Lai, Ming-Hsin, Chen, Liang-Jwu, Jena, Kshirod K., Hechanova, Sherry Lou, Ke, Ting-Jyun, Chiu, Tai-Yuan, Tsai, Zong-Yuan, Chen, Guo-Kai, Tsai, Kuan-Chieh, Leu, Wei-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863282/
https://www.ncbi.nlm.nih.gov/pubmed/36675314
http://dx.doi.org/10.3390/ijms24021798
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author Li, Charng-Pei
Wu, Dong-Hong
Huang, Shou-Horng
Meng, Menghsiao
Shih, Hsien-Tzung
Lai, Ming-Hsin
Chen, Liang-Jwu
Jena, Kshirod K.
Hechanova, Sherry Lou
Ke, Ting-Jyun
Chiu, Tai-Yuan
Tsai, Zong-Yuan
Chen, Guo-Kai
Tsai, Kuan-Chieh
Leu, Wei-Ming
author_facet Li, Charng-Pei
Wu, Dong-Hong
Huang, Shou-Horng
Meng, Menghsiao
Shih, Hsien-Tzung
Lai, Ming-Hsin
Chen, Liang-Jwu
Jena, Kshirod K.
Hechanova, Sherry Lou
Ke, Ting-Jyun
Chiu, Tai-Yuan
Tsai, Zong-Yuan
Chen, Guo-Kai
Tsai, Kuan-Chieh
Leu, Wei-Ming
author_sort Li, Charng-Pei
collection PubMed
description Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara (IRGC 102165) near the centromere of chromosome 4. We compared the NIL (TNG71-Bph45) and the recurrent parent to explore how the Bph45 gene confers BPH resistance. We found that TNG71-Bph45 is less attractive to BPH at least partially because it produces less limonene. Chiral analysis revealed that the major form of limonene in both rice lines was the L-form. However, both L- and D-limonene attracted BPH when applied exogenously to TNG71-Bph45 rice. The transcript amounts of limonene synthase were significantly higher in TNG71 than in TNG71-Bph45 and were induced by BPH infestation only in the former. Introgression of the Bph45 gene into another japonica variety, Tainan 11, also resulted in a low limonene content. Moreover, several dominantly acting BPH resistance genes introduced into the BPH-sensitive IR24 line compromised its limonene-producing ability and concurrently decreased its attractiveness to BPH. These observations suggest that reducing limonene production may be a common resistance strategy against BPH in rice.
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spelling pubmed-98632822023-01-22 The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene Li, Charng-Pei Wu, Dong-Hong Huang, Shou-Horng Meng, Menghsiao Shih, Hsien-Tzung Lai, Ming-Hsin Chen, Liang-Jwu Jena, Kshirod K. Hechanova, Sherry Lou Ke, Ting-Jyun Chiu, Tai-Yuan Tsai, Zong-Yuan Chen, Guo-Kai Tsai, Kuan-Chieh Leu, Wei-Ming Int J Mol Sci Article Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara (IRGC 102165) near the centromere of chromosome 4. We compared the NIL (TNG71-Bph45) and the recurrent parent to explore how the Bph45 gene confers BPH resistance. We found that TNG71-Bph45 is less attractive to BPH at least partially because it produces less limonene. Chiral analysis revealed that the major form of limonene in both rice lines was the L-form. However, both L- and D-limonene attracted BPH when applied exogenously to TNG71-Bph45 rice. The transcript amounts of limonene synthase were significantly higher in TNG71 than in TNG71-Bph45 and were induced by BPH infestation only in the former. Introgression of the Bph45 gene into another japonica variety, Tainan 11, also resulted in a low limonene content. Moreover, several dominantly acting BPH resistance genes introduced into the BPH-sensitive IR24 line compromised its limonene-producing ability and concurrently decreased its attractiveness to BPH. These observations suggest that reducing limonene production may be a common resistance strategy against BPH in rice. MDPI 2023-01-16 /pmc/articles/PMC9863282/ /pubmed/36675314 http://dx.doi.org/10.3390/ijms24021798 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Charng-Pei
Wu, Dong-Hong
Huang, Shou-Horng
Meng, Menghsiao
Shih, Hsien-Tzung
Lai, Ming-Hsin
Chen, Liang-Jwu
Jena, Kshirod K.
Hechanova, Sherry Lou
Ke, Ting-Jyun
Chiu, Tai-Yuan
Tsai, Zong-Yuan
Chen, Guo-Kai
Tsai, Kuan-Chieh
Leu, Wei-Ming
The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene
title The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene
title_full The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene
title_fullStr The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene
title_full_unstemmed The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene
title_short The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene
title_sort bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863282/
https://www.ncbi.nlm.nih.gov/pubmed/36675314
http://dx.doi.org/10.3390/ijms24021798
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