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Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster

Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despit...

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Autores principales: González, Marco Antonio Cabrera, Gonçalves, Ana Alice Maia, Ottino, Jennifer, Leite, Jaqueline Costa, Resende, Lucilene Aparecida, Melo-Júnior, Otoni Alves, Silveira, Patrícia, Cardoso, Mariana Santos, Fujiwara, Ricardo Toshio, Bueno, Lilian Lacerda, Santos, Renato Lima, de Carvalho, Tatiane Furtado, Garcia, Giani Martins, Paes, Paulo Ricardo de Oliveira, Galdino, Alexsandro Sobreira, Chávez-Fumagalli, Miguel Angel, Melo, Marília Martins, Silveira-Lemos, Denise, Martins-Filho, Olindo Assis, Dutra, Walderez Ornelas, Mosqueira, Vanessa Carla Furtado, Giunchetti, Rodolfo Cordeiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863486/
https://www.ncbi.nlm.nih.gov/pubmed/36679956
http://dx.doi.org/10.3390/vaccines11010111
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author González, Marco Antonio Cabrera
Gonçalves, Ana Alice Maia
Ottino, Jennifer
Leite, Jaqueline Costa
Resende, Lucilene Aparecida
Melo-Júnior, Otoni Alves
Silveira, Patrícia
Cardoso, Mariana Santos
Fujiwara, Ricardo Toshio
Bueno, Lilian Lacerda
Santos, Renato Lima
de Carvalho, Tatiane Furtado
Garcia, Giani Martins
Paes, Paulo Ricardo de Oliveira
Galdino, Alexsandro Sobreira
Chávez-Fumagalli, Miguel Angel
Melo, Marília Martins
Silveira-Lemos, Denise
Martins-Filho, Olindo Assis
Dutra, Walderez Ornelas
Mosqueira, Vanessa Carla Furtado
Giunchetti, Rodolfo Cordeiro
author_facet González, Marco Antonio Cabrera
Gonçalves, Ana Alice Maia
Ottino, Jennifer
Leite, Jaqueline Costa
Resende, Lucilene Aparecida
Melo-Júnior, Otoni Alves
Silveira, Patrícia
Cardoso, Mariana Santos
Fujiwara, Ricardo Toshio
Bueno, Lilian Lacerda
Santos, Renato Lima
de Carvalho, Tatiane Furtado
Garcia, Giani Martins
Paes, Paulo Ricardo de Oliveira
Galdino, Alexsandro Sobreira
Chávez-Fumagalli, Miguel Angel
Melo, Marília Martins
Silveira-Lemos, Denise
Martins-Filho, Olindo Assis
Dutra, Walderez Ornelas
Mosqueira, Vanessa Carla Furtado
Giunchetti, Rodolfo Cordeiro
author_sort González, Marco Antonio Cabrera
collection PubMed
description Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, (poly (D, L-lactic) acid (PLA) polymer) loading Leishmania amazonensis antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-Leishmania IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups showed an intense reduction in liver and spleen parasitic load by qPCR. The LAPSmP and LAPSmG vaccines showed exceptional results, indicating that they may be promising candidates as a VL vaccine.
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spelling pubmed-98634862023-01-22 Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster González, Marco Antonio Cabrera Gonçalves, Ana Alice Maia Ottino, Jennifer Leite, Jaqueline Costa Resende, Lucilene Aparecida Melo-Júnior, Otoni Alves Silveira, Patrícia Cardoso, Mariana Santos Fujiwara, Ricardo Toshio Bueno, Lilian Lacerda Santos, Renato Lima de Carvalho, Tatiane Furtado Garcia, Giani Martins Paes, Paulo Ricardo de Oliveira Galdino, Alexsandro Sobreira Chávez-Fumagalli, Miguel Angel Melo, Marília Martins Silveira-Lemos, Denise Martins-Filho, Olindo Assis Dutra, Walderez Ornelas Mosqueira, Vanessa Carla Furtado Giunchetti, Rodolfo Cordeiro Vaccines (Basel) Article Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, (poly (D, L-lactic) acid (PLA) polymer) loading Leishmania amazonensis antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-Leishmania IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups showed an intense reduction in liver and spleen parasitic load by qPCR. The LAPSmP and LAPSmG vaccines showed exceptional results, indicating that they may be promising candidates as a VL vaccine. MDPI 2023-01-02 /pmc/articles/PMC9863486/ /pubmed/36679956 http://dx.doi.org/10.3390/vaccines11010111 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
González, Marco Antonio Cabrera
Gonçalves, Ana Alice Maia
Ottino, Jennifer
Leite, Jaqueline Costa
Resende, Lucilene Aparecida
Melo-Júnior, Otoni Alves
Silveira, Patrícia
Cardoso, Mariana Santos
Fujiwara, Ricardo Toshio
Bueno, Lilian Lacerda
Santos, Renato Lima
de Carvalho, Tatiane Furtado
Garcia, Giani Martins
Paes, Paulo Ricardo de Oliveira
Galdino, Alexsandro Sobreira
Chávez-Fumagalli, Miguel Angel
Melo, Marília Martins
Silveira-Lemos, Denise
Martins-Filho, Olindo Assis
Dutra, Walderez Ornelas
Mosqueira, Vanessa Carla Furtado
Giunchetti, Rodolfo Cordeiro
Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster
title Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster
title_full Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster
title_fullStr Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster
title_full_unstemmed Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster
title_short Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster
title_sort vaccination with formulation of nanoparticles loaded with leishmania amazonensis antigens confers protection against experimental visceral leishmaniasis in hamster
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863486/
https://www.ncbi.nlm.nih.gov/pubmed/36679956
http://dx.doi.org/10.3390/vaccines11010111
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