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Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology

Ten jatrophane diterpenoids were isolated from the whole plant Euphorbia peplus Linn. including seven new ones, named euphjatrophanes A-G (labeled compounds 1, 2, 4–8). Their structures were elucidated with a combination of spectroscopic and single-crystal X-ray crystallography, enabling the identif...

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Autores principales: Yan, Ying, Zhou, Qi, Ran, Xiaoqian, Lu, Qingyun, Zhang, Cuishan, Peng, Mingyou, Tang, Lei, Luo, Rongcan, Di, Yingtong, Hao, Xiaojiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863522/
https://www.ncbi.nlm.nih.gov/pubmed/36674604
http://dx.doi.org/10.3390/ijms24021088
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author Yan, Ying
Zhou, Qi
Ran, Xiaoqian
Lu, Qingyun
Zhang, Cuishan
Peng, Mingyou
Tang, Lei
Luo, Rongcan
Di, Yingtong
Hao, Xiaojiang
author_facet Yan, Ying
Zhou, Qi
Ran, Xiaoqian
Lu, Qingyun
Zhang, Cuishan
Peng, Mingyou
Tang, Lei
Luo, Rongcan
Di, Yingtong
Hao, Xiaojiang
author_sort Yan, Ying
collection PubMed
description Ten jatrophane diterpenoids were isolated from the whole plant Euphorbia peplus Linn. including seven new ones, named euphjatrophanes A-G (labeled compounds 1, 2, 4–8). Their structures were elucidated with a combination of spectroscopic and single-crystal X-ray crystallography, enabling the identification of compounds 3, 9, and 10 as the previously published euphpepluones G, K, and L, respectively. All compounds were evaluated for their bioactivity with flow cytometry in assays of autophagic flux in HM Cherry-GFP-LC3 (human microglia cells stably expressing the tandem monomeric mCherry-GFP-tagged LC3) cells. Euphpepluone K (9) significantly activated autophagic flux, an effect that was verified with confocal analysis. Moreover, cellular assays showed that euphpepluone K (9) induced autophagy and inhibited Tau pathology.
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spelling pubmed-98635222023-01-22 Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology Yan, Ying Zhou, Qi Ran, Xiaoqian Lu, Qingyun Zhang, Cuishan Peng, Mingyou Tang, Lei Luo, Rongcan Di, Yingtong Hao, Xiaojiang Int J Mol Sci Article Ten jatrophane diterpenoids were isolated from the whole plant Euphorbia peplus Linn. including seven new ones, named euphjatrophanes A-G (labeled compounds 1, 2, 4–8). Their structures were elucidated with a combination of spectroscopic and single-crystal X-ray crystallography, enabling the identification of compounds 3, 9, and 10 as the previously published euphpepluones G, K, and L, respectively. All compounds were evaluated for their bioactivity with flow cytometry in assays of autophagic flux in HM Cherry-GFP-LC3 (human microglia cells stably expressing the tandem monomeric mCherry-GFP-tagged LC3) cells. Euphpepluone K (9) significantly activated autophagic flux, an effect that was verified with confocal analysis. Moreover, cellular assays showed that euphpepluone K (9) induced autophagy and inhibited Tau pathology. MDPI 2023-01-06 /pmc/articles/PMC9863522/ /pubmed/36674604 http://dx.doi.org/10.3390/ijms24021088 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Ying
Zhou, Qi
Ran, Xiaoqian
Lu, Qingyun
Zhang, Cuishan
Peng, Mingyou
Tang, Lei
Luo, Rongcan
Di, Yingtong
Hao, Xiaojiang
Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology
title Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology
title_full Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology
title_fullStr Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology
title_full_unstemmed Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology
title_short Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology
title_sort jatrophane diterpenoids from euphorbia peplus linn. as activators of autophagy and inhibitors of tau pathology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863522/
https://www.ncbi.nlm.nih.gov/pubmed/36674604
http://dx.doi.org/10.3390/ijms24021088
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