Cargando…

The Chemotherapeutic Efficacy of Hyaluronic Acid Coated Polymeric Nanoparticles against Breast Cancer Metastasis in Female NCr-Nu/Nu Nude Mice

Polyethylene glycol (PEG) coated Poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for cancer treatment are biocompatible, nonimmunogenic and accumulate in tumour sites due to the enhanced permeability and retention (EPR). Doxorubicin (DOX) is a potent but cardiotoxic anticancer agent. Hyaluro...

Descripción completa

Detalles Bibliográficos
Autores principales: Almoustafa, Hassan A., Alshawsh, Mohammed Abdullah, Al-Suede, Fouad Saleih R., Alshehade, Salah Abdulrazak, Abdul Majid, Amin Malik Shah, Chik, Zamri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863707/
https://www.ncbi.nlm.nih.gov/pubmed/36679166
http://dx.doi.org/10.3390/polym15020284
_version_ 1784875401408937984
author Almoustafa, Hassan A.
Alshawsh, Mohammed Abdullah
Al-Suede, Fouad Saleih R.
Alshehade, Salah Abdulrazak
Abdul Majid, Amin Malik Shah
Chik, Zamri
author_facet Almoustafa, Hassan A.
Alshawsh, Mohammed Abdullah
Al-Suede, Fouad Saleih R.
Alshehade, Salah Abdulrazak
Abdul Majid, Amin Malik Shah
Chik, Zamri
author_sort Almoustafa, Hassan A.
collection PubMed
description Polyethylene glycol (PEG) coated Poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for cancer treatment are biocompatible, nonimmunogenic and accumulate in tumour sites due to the enhanced permeability and retention (EPR). Doxorubicin (DOX) is a potent but cardiotoxic anticancer agent. Hyaluronic acid (HA) occurs naturally in the extra-cellar matrix and binds to CD44 receptors which are overexpressed in cancer metastasis, proven to be characteristic of cancer stem cells and responsible for multidrug resistance. In this study, an athymic mice model of breast cancer metastasis was developed using red fluorescent protein (RFP)-labelled triple negative cancer cells. The animals were divided into four treatment groups (Control, HA-PEG-PLGA nanoparticles, PEG-PLGA nanoparticles, and Free DOX). The tumour size growth was assessed until day 25 when animals were sacrificed. Mice treated with HA-PEG-PLGA NPs inhibited tumour growth. The tumour growth at day 25 (118% ± 13.0) was significantly (p < 0.05) less than PEG-PLGA NPs (376% ± 590 and control (826% ± 970). Fluorescent microscopy revealed that HA-PEG-PLGA NPs had significantly (p < 0.05) less metastasis in liver, spleen, colon, and lungs as compared to control and to Free DOX groups. The efficacy of HA-PEG-PLGA NPs was proven in vivo. Further pharmacokinetic and toxicity studies are required for this formulation to be ready for clinical research.
format Online
Article
Text
id pubmed-9863707
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98637072023-01-22 The Chemotherapeutic Efficacy of Hyaluronic Acid Coated Polymeric Nanoparticles against Breast Cancer Metastasis in Female NCr-Nu/Nu Nude Mice Almoustafa, Hassan A. Alshawsh, Mohammed Abdullah Al-Suede, Fouad Saleih R. Alshehade, Salah Abdulrazak Abdul Majid, Amin Malik Shah Chik, Zamri Polymers (Basel) Article Polyethylene glycol (PEG) coated Poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for cancer treatment are biocompatible, nonimmunogenic and accumulate in tumour sites due to the enhanced permeability and retention (EPR). Doxorubicin (DOX) is a potent but cardiotoxic anticancer agent. Hyaluronic acid (HA) occurs naturally in the extra-cellar matrix and binds to CD44 receptors which are overexpressed in cancer metastasis, proven to be characteristic of cancer stem cells and responsible for multidrug resistance. In this study, an athymic mice model of breast cancer metastasis was developed using red fluorescent protein (RFP)-labelled triple negative cancer cells. The animals were divided into four treatment groups (Control, HA-PEG-PLGA nanoparticles, PEG-PLGA nanoparticles, and Free DOX). The tumour size growth was assessed until day 25 when animals were sacrificed. Mice treated with HA-PEG-PLGA NPs inhibited tumour growth. The tumour growth at day 25 (118% ± 13.0) was significantly (p < 0.05) less than PEG-PLGA NPs (376% ± 590 and control (826% ± 970). Fluorescent microscopy revealed that HA-PEG-PLGA NPs had significantly (p < 0.05) less metastasis in liver, spleen, colon, and lungs as compared to control and to Free DOX groups. The efficacy of HA-PEG-PLGA NPs was proven in vivo. Further pharmacokinetic and toxicity studies are required for this formulation to be ready for clinical research. MDPI 2023-01-05 /pmc/articles/PMC9863707/ /pubmed/36679166 http://dx.doi.org/10.3390/polym15020284 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Almoustafa, Hassan A.
Alshawsh, Mohammed Abdullah
Al-Suede, Fouad Saleih R.
Alshehade, Salah Abdulrazak
Abdul Majid, Amin Malik Shah
Chik, Zamri
The Chemotherapeutic Efficacy of Hyaluronic Acid Coated Polymeric Nanoparticles against Breast Cancer Metastasis in Female NCr-Nu/Nu Nude Mice
title The Chemotherapeutic Efficacy of Hyaluronic Acid Coated Polymeric Nanoparticles against Breast Cancer Metastasis in Female NCr-Nu/Nu Nude Mice
title_full The Chemotherapeutic Efficacy of Hyaluronic Acid Coated Polymeric Nanoparticles against Breast Cancer Metastasis in Female NCr-Nu/Nu Nude Mice
title_fullStr The Chemotherapeutic Efficacy of Hyaluronic Acid Coated Polymeric Nanoparticles against Breast Cancer Metastasis in Female NCr-Nu/Nu Nude Mice
title_full_unstemmed The Chemotherapeutic Efficacy of Hyaluronic Acid Coated Polymeric Nanoparticles against Breast Cancer Metastasis in Female NCr-Nu/Nu Nude Mice
title_short The Chemotherapeutic Efficacy of Hyaluronic Acid Coated Polymeric Nanoparticles against Breast Cancer Metastasis in Female NCr-Nu/Nu Nude Mice
title_sort chemotherapeutic efficacy of hyaluronic acid coated polymeric nanoparticles against breast cancer metastasis in female ncr-nu/nu nude mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863707/
https://www.ncbi.nlm.nih.gov/pubmed/36679166
http://dx.doi.org/10.3390/polym15020284
work_keys_str_mv AT almoustafahassana thechemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT alshawshmohammedabdullah thechemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT alsuedefouadsaleihr thechemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT alshehadesalahabdulrazak thechemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT abdulmajidaminmalikshah thechemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT chikzamri thechemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT almoustafahassana chemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT alshawshmohammedabdullah chemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT alsuedefouadsaleihr chemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT alshehadesalahabdulrazak chemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT abdulmajidaminmalikshah chemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice
AT chikzamri chemotherapeuticefficacyofhyaluronicacidcoatedpolymericnanoparticlesagainstbreastcancermetastasisinfemalencrnununudemice