Cargando…

Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern

The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spawned an ongoing demand for new research reagents and interventions. Herein we describe a panel of monoclonal antibodies raised against SARS-CoV-2. One antibody sh...

Descripción completa

Detalles Bibliográficos
Autores principales: Morgan, Mahali S., Yan, Kexin, Le, Thuy T., Johnston, Ryan A., Amarilla, Alberto A., Muller, David A., McMillan, Christopher L. D., Modhiran, Naphak, Watterson, Daniel, Potter, James R., Sng, Julian D.J., Lor, Mary, Paramitha, Devina, Isaacs, Ariel, Khromykh, Alexander A., Hall, Roy A., Suhrbier, Andreas, Rawle, Daniel J., Hobson-Peters, Jody
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863740/
https://www.ncbi.nlm.nih.gov/pubmed/36680179
http://dx.doi.org/10.3390/v15010139
_version_ 1784875409622433792
author Morgan, Mahali S.
Yan, Kexin
Le, Thuy T.
Johnston, Ryan A.
Amarilla, Alberto A.
Muller, David A.
McMillan, Christopher L. D.
Modhiran, Naphak
Watterson, Daniel
Potter, James R.
Sng, Julian D.J.
Lor, Mary
Paramitha, Devina
Isaacs, Ariel
Khromykh, Alexander A.
Hall, Roy A.
Suhrbier, Andreas
Rawle, Daniel J.
Hobson-Peters, Jody
author_facet Morgan, Mahali S.
Yan, Kexin
Le, Thuy T.
Johnston, Ryan A.
Amarilla, Alberto A.
Muller, David A.
McMillan, Christopher L. D.
Modhiran, Naphak
Watterson, Daniel
Potter, James R.
Sng, Julian D.J.
Lor, Mary
Paramitha, Devina
Isaacs, Ariel
Khromykh, Alexander A.
Hall, Roy A.
Suhrbier, Andreas
Rawle, Daniel J.
Hobson-Peters, Jody
author_sort Morgan, Mahali S.
collection PubMed
description The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spawned an ongoing demand for new research reagents and interventions. Herein we describe a panel of monoclonal antibodies raised against SARS-CoV-2. One antibody showed excellent utility for immunohistochemistry, clearly staining infected cells in formalin-fixed and paraffin embedded lungs and brains of mice infected with the original and the omicron variants of SARS-CoV-2. We demonstrate the reactivity to multiple variants of concern using ELISAs and describe the use of the antibodies in indirect immunofluorescence assays, Western blots, and rapid antigen tests. Finally, we illustrate the ability of two antibodies to reduce significantly viral tissue titers in K18-hACE2 transgenic mice infected with the original and an omicron isolate of SARS-CoV-2.
format Online
Article
Text
id pubmed-9863740
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98637402023-01-22 Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern Morgan, Mahali S. Yan, Kexin Le, Thuy T. Johnston, Ryan A. Amarilla, Alberto A. Muller, David A. McMillan, Christopher L. D. Modhiran, Naphak Watterson, Daniel Potter, James R. Sng, Julian D.J. Lor, Mary Paramitha, Devina Isaacs, Ariel Khromykh, Alexander A. Hall, Roy A. Suhrbier, Andreas Rawle, Daniel J. Hobson-Peters, Jody Viruses Article The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spawned an ongoing demand for new research reagents and interventions. Herein we describe a panel of monoclonal antibodies raised against SARS-CoV-2. One antibody showed excellent utility for immunohistochemistry, clearly staining infected cells in formalin-fixed and paraffin embedded lungs and brains of mice infected with the original and the omicron variants of SARS-CoV-2. We demonstrate the reactivity to multiple variants of concern using ELISAs and describe the use of the antibodies in indirect immunofluorescence assays, Western blots, and rapid antigen tests. Finally, we illustrate the ability of two antibodies to reduce significantly viral tissue titers in K18-hACE2 transgenic mice infected with the original and an omicron isolate of SARS-CoV-2. MDPI 2022-12-31 /pmc/articles/PMC9863740/ /pubmed/36680179 http://dx.doi.org/10.3390/v15010139 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morgan, Mahali S.
Yan, Kexin
Le, Thuy T.
Johnston, Ryan A.
Amarilla, Alberto A.
Muller, David A.
McMillan, Christopher L. D.
Modhiran, Naphak
Watterson, Daniel
Potter, James R.
Sng, Julian D.J.
Lor, Mary
Paramitha, Devina
Isaacs, Ariel
Khromykh, Alexander A.
Hall, Roy A.
Suhrbier, Andreas
Rawle, Daniel J.
Hobson-Peters, Jody
Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
title Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
title_full Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
title_fullStr Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
title_full_unstemmed Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
title_short Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
title_sort monoclonal antibodies specific for sars-cov-2 spike protein suitable for multiple applications for current variants of concern
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863740/
https://www.ncbi.nlm.nih.gov/pubmed/36680179
http://dx.doi.org/10.3390/v15010139
work_keys_str_mv AT morganmahalis monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT yankexin monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT lethuyt monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT johnstonryana monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT amarillaalbertoa monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT mullerdavida monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT mcmillanchristopherld monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT modhirannaphak monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT wattersondaniel monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT potterjamesr monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT sngjuliandj monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT lormary monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT paramithadevina monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT isaacsariel monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT khromykhalexandera monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT hallroya monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT suhrbierandreas monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT rawledanielj monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern
AT hobsonpetersjody monoclonalantibodiesspecificforsarscov2spikeproteinsuitableformultipleapplicationsforcurrentvariantsofconcern