Determination of Free Histidine in Complex Hair Care Products with Minimum Sample Preparation Using Cation-Exchange Chromatography and Post Column Derivatization

In this communication, we describe the first analytical method for the determination of free histidine in hair care products (shampoos and conditioners). Cation-exchange chromatography combined with postcolumn derivatization and fluorimetric detection enabled the accurate (recovery: 83.5–114.8%) and...

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Detalles Bibliográficos
Autores principales: Tsiasioti, Apostolia, Zacharis, Constantinos K., Tzanavaras, Paraskevas D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863815/
https://www.ncbi.nlm.nih.gov/pubmed/36677945
http://dx.doi.org/10.3390/molecules28020888
Descripción
Sumario:In this communication, we describe the first analytical method for the determination of free histidine in hair care products (shampoos and conditioners). Cation-exchange chromatography combined with postcolumn derivatization and fluorimetric detection enabled the accurate (recovery: 83.5–114.8%) and precise (2.4–5.6% RSD) determination of free histidine without matrix interferences at concentration levels down to 1.5 mg kg(−1). Real commercially available samples were found to contain the amino acid at levels ranging between 70 and 535 mg kg(−1).

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