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Rosmarinic Acid Production from Origanum dictamnus L. Root Liquid Cultures In Vitro
In the present work Origanum dictamnus L. was studied as a suitable in vitro adventitious root culture system for the production of important bioactive molecules, such as rosmarinic acid (RA). Callus culture was initiated from leaf, petiole and root explants on solid MS medium supplemented with eith...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864065/ https://www.ncbi.nlm.nih.gov/pubmed/36679010 http://dx.doi.org/10.3390/plants12020299 |
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author | Sarropoulou, Virginia Paloukopoulou, Charikleia Karioti, Anastasia Maloupa, Eleni Grigoriadou, Katerina |
author_facet | Sarropoulou, Virginia Paloukopoulou, Charikleia Karioti, Anastasia Maloupa, Eleni Grigoriadou, Katerina |
author_sort | Sarropoulou, Virginia |
collection | PubMed |
description | In the present work Origanum dictamnus L. was studied as a suitable in vitro adventitious root culture system for the production of important bioactive molecules, such as rosmarinic acid (RA). Callus culture was initiated from leaf, petiole and root explants on solid MS medium supplemented with either 5 μM NAA + 5 μM kinetin (ODK3) or 5 μM NAA + 0.5 μM kinetin (ODK4). New roots formed from leaf, petiole and root calluses were aseptically transferred into Erlenmeyer flasks containing 100 mL liquid medium and shaken at 120 rpm in the dark. The liquid medium used was the MS supplemented either with 35 μM IBA + 2.5 μM kinetin (ODY1) or 5 μM NAA + 0.5 μM kinetin (ODY2). Biomass production parameters, RA content (%) and yield index (YI) were recorded for each treatment explant type, medium composition and incubation period. Results showed, in every case, the production of RA in vitro. Between the two liquid media (ODY1, ODY2) and the different culture periods, the ODY1 medium and the longest 200-day-culture period were more effective for RA and biomass production, regardless of the initial explant type used. The combination of ODK4-ODY1 resulted in higher RA (5.1% and 4.7%), fresh biomass production (19.0 g and 11.6 g), mean YI (93.7 mg and 51.4 mg) and YI per explant (3.75 mg and 2.06 mg) for roots derived from leaf calluses and root calluses, respectively. However, the solid ODK3 (200 days)–liquid ODY1 (40 days) transition treatment was more beneficial for roots derived from petiole calluses leading to an 18.8-fold increase in fresh biomass growth rate. RA accumulation and YIs were also significantly influenced by explant type, with the highest value produced from root petiole calluses (6.6% RA dry weight, 115.3 mg mean YI and 4.61 mg YI per explant) after 240 days. |
format | Online Article Text |
id | pubmed-9864065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98640652023-01-22 Rosmarinic Acid Production from Origanum dictamnus L. Root Liquid Cultures In Vitro Sarropoulou, Virginia Paloukopoulou, Charikleia Karioti, Anastasia Maloupa, Eleni Grigoriadou, Katerina Plants (Basel) Article In the present work Origanum dictamnus L. was studied as a suitable in vitro adventitious root culture system for the production of important bioactive molecules, such as rosmarinic acid (RA). Callus culture was initiated from leaf, petiole and root explants on solid MS medium supplemented with either 5 μM NAA + 5 μM kinetin (ODK3) or 5 μM NAA + 0.5 μM kinetin (ODK4). New roots formed from leaf, petiole and root calluses were aseptically transferred into Erlenmeyer flasks containing 100 mL liquid medium and shaken at 120 rpm in the dark. The liquid medium used was the MS supplemented either with 35 μM IBA + 2.5 μM kinetin (ODY1) or 5 μM NAA + 0.5 μM kinetin (ODY2). Biomass production parameters, RA content (%) and yield index (YI) were recorded for each treatment explant type, medium composition and incubation period. Results showed, in every case, the production of RA in vitro. Between the two liquid media (ODY1, ODY2) and the different culture periods, the ODY1 medium and the longest 200-day-culture period were more effective for RA and biomass production, regardless of the initial explant type used. The combination of ODK4-ODY1 resulted in higher RA (5.1% and 4.7%), fresh biomass production (19.0 g and 11.6 g), mean YI (93.7 mg and 51.4 mg) and YI per explant (3.75 mg and 2.06 mg) for roots derived from leaf calluses and root calluses, respectively. However, the solid ODK3 (200 days)–liquid ODY1 (40 days) transition treatment was more beneficial for roots derived from petiole calluses leading to an 18.8-fold increase in fresh biomass growth rate. RA accumulation and YIs were also significantly influenced by explant type, with the highest value produced from root petiole calluses (6.6% RA dry weight, 115.3 mg mean YI and 4.61 mg YI per explant) after 240 days. MDPI 2023-01-08 /pmc/articles/PMC9864065/ /pubmed/36679010 http://dx.doi.org/10.3390/plants12020299 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sarropoulou, Virginia Paloukopoulou, Charikleia Karioti, Anastasia Maloupa, Eleni Grigoriadou, Katerina Rosmarinic Acid Production from Origanum dictamnus L. Root Liquid Cultures In Vitro |
title | Rosmarinic Acid Production from Origanum dictamnus L. Root Liquid Cultures In Vitro |
title_full | Rosmarinic Acid Production from Origanum dictamnus L. Root Liquid Cultures In Vitro |
title_fullStr | Rosmarinic Acid Production from Origanum dictamnus L. Root Liquid Cultures In Vitro |
title_full_unstemmed | Rosmarinic Acid Production from Origanum dictamnus L. Root Liquid Cultures In Vitro |
title_short | Rosmarinic Acid Production from Origanum dictamnus L. Root Liquid Cultures In Vitro |
title_sort | rosmarinic acid production from origanum dictamnus l. root liquid cultures in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864065/ https://www.ncbi.nlm.nih.gov/pubmed/36679010 http://dx.doi.org/10.3390/plants12020299 |
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