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Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey

Honey is considered to be a health-promoting food product. Therefore, it is assumed that it should be free of contaminants. Although the use of organochlorine pesticides (OCPs) was banned a few decades ago in developed countries, persistent organic pollutants (POPs) are still detected in various env...

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Autores principales: Lewiński, Radosław, Hernik, Agnieszka, Liszewska, Monika, Buckley, Brian, Czaja, Katarzyna, Korcz, Wojciech, Słomczyńska, Anna, Struciński, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864146/
https://www.ncbi.nlm.nih.gov/pubmed/36677901
http://dx.doi.org/10.3390/molecules28020842
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author Lewiński, Radosław
Hernik, Agnieszka
Liszewska, Monika
Buckley, Brian
Czaja, Katarzyna
Korcz, Wojciech
Słomczyńska, Anna
Struciński, Paweł
author_facet Lewiński, Radosław
Hernik, Agnieszka
Liszewska, Monika
Buckley, Brian
Czaja, Katarzyna
Korcz, Wojciech
Słomczyńska, Anna
Struciński, Paweł
author_sort Lewiński, Radosław
collection PubMed
description Honey is considered to be a health-promoting food product. Therefore, it is assumed that it should be free of contaminants. Although the use of organochlorine pesticides (OCPs) was banned a few decades ago in developed countries, persistent organic pollutants (POPs) are still detected in various environmental and biological matrices, including food. These contaminants exhibit toxic properties and bioaccumulate in some food chains. The validation of a modified QuEChERS extraction method was successfully performed for o,p’-DDT, o,p’-DDE, o,p’-DDD, p,p’-DDT, p,p’-DDE, p,p’-DDD, heptachlor and dieldrin. 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB 153) was used as an internal standard. The modification involved changing the solvent from acetonitrile to n-hexane after extraction. Quantitation was carried out using gas chromatography with an electron capture detector (µECD). The mean recovery values for o,p’-DDT, o,p’-DDE, o,p’-DDD, p,p’-DDT, p,p’-DDE, p,p’-DDD and dieldrin, spiked at 2.9 ng/g and 20 ng/g, ranged from 64.7% to 129.3%, and, for heptachlor spiked at 5.6 ng/g and 20 ng/g, ranged from 68.0% to 88.3%. The relative standard deviation (RSD) for these concentrations did not exceed 20%, and the within-laboratory reproducibility was below 20%, except o,p’-DDE and p,p’-DDT, which were 25.2% and 20.7%, respectively. This modified QuEChERS extraction method for selected organochlorine compounds was demonstrated as effective for routine testing in honey.
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spelling pubmed-98641462023-01-22 Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey Lewiński, Radosław Hernik, Agnieszka Liszewska, Monika Buckley, Brian Czaja, Katarzyna Korcz, Wojciech Słomczyńska, Anna Struciński, Paweł Molecules Article Honey is considered to be a health-promoting food product. Therefore, it is assumed that it should be free of contaminants. Although the use of organochlorine pesticides (OCPs) was banned a few decades ago in developed countries, persistent organic pollutants (POPs) are still detected in various environmental and biological matrices, including food. These contaminants exhibit toxic properties and bioaccumulate in some food chains. The validation of a modified QuEChERS extraction method was successfully performed for o,p’-DDT, o,p’-DDE, o,p’-DDD, p,p’-DDT, p,p’-DDE, p,p’-DDD, heptachlor and dieldrin. 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB 153) was used as an internal standard. The modification involved changing the solvent from acetonitrile to n-hexane after extraction. Quantitation was carried out using gas chromatography with an electron capture detector (µECD). The mean recovery values for o,p’-DDT, o,p’-DDE, o,p’-DDD, p,p’-DDT, p,p’-DDE, p,p’-DDD and dieldrin, spiked at 2.9 ng/g and 20 ng/g, ranged from 64.7% to 129.3%, and, for heptachlor spiked at 5.6 ng/g and 20 ng/g, ranged from 68.0% to 88.3%. The relative standard deviation (RSD) for these concentrations did not exceed 20%, and the within-laboratory reproducibility was below 20%, except o,p’-DDE and p,p’-DDT, which were 25.2% and 20.7%, respectively. This modified QuEChERS extraction method for selected organochlorine compounds was demonstrated as effective for routine testing in honey. MDPI 2023-01-14 /pmc/articles/PMC9864146/ /pubmed/36677901 http://dx.doi.org/10.3390/molecules28020842 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lewiński, Radosław
Hernik, Agnieszka
Liszewska, Monika
Buckley, Brian
Czaja, Katarzyna
Korcz, Wojciech
Słomczyńska, Anna
Struciński, Paweł
Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey
title Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey
title_full Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey
title_fullStr Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey
title_full_unstemmed Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey
title_short Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey
title_sort validation of a modified quechers method for the determination of selected organochlorine compounds in honey
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864146/
https://www.ncbi.nlm.nih.gov/pubmed/36677901
http://dx.doi.org/10.3390/molecules28020842
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