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Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection

Old world alphaviruses, such as Ross River virus (RRV), cause debilitating arthralgia during acute and chronic stages of the disease. RRV-induced cartilage degradation has been implicated as a cause of joint pain felt by RRV patients. Chondrocytes are a major cell type of cartilage and are involved...

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Autores principales: Lim, Elisa X. Y., Webster, Julie A., Rudd, Penny A., Herrero, Lara J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864161/
https://www.ncbi.nlm.nih.gov/pubmed/36680176
http://dx.doi.org/10.3390/v15010136
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author Lim, Elisa X. Y.
Webster, Julie A.
Rudd, Penny A.
Herrero, Lara J.
author_facet Lim, Elisa X. Y.
Webster, Julie A.
Rudd, Penny A.
Herrero, Lara J.
author_sort Lim, Elisa X. Y.
collection PubMed
description Old world alphaviruses, such as Ross River virus (RRV), cause debilitating arthralgia during acute and chronic stages of the disease. RRV-induced cartilage degradation has been implicated as a cause of joint pain felt by RRV patients. Chondrocytes are a major cell type of cartilage and are involved in the production and maintenance of the cartilage matrix. It is thought that these cells may play a vital role in RRV disease pathogenesis. In this study, we used RNA-sequencing (RNA-Seq) to examine the transcriptomes of RRV-infected and bystander chondrocytes in the same environment. RRV containing green fluorescent protein (GFP) allowed for the separation of RRV-infected (GFP+) and bystander uninfected cells (GFP−). We found that whereas GFP+ and GFP− populations commonly presented similar gene expression profiles during infection, there were also unique signatures. For example, RIMS2 and FOXJ1 were unique to GFP+ cells, whilst Aim2 and CCL8 were only found in bystander chondrocytes. This indicates that careful selection of potential therapeutic targets is important to minimise adverse effects to the neighbouring uninfected cell populations. Our study serves as a resource to provide more information about the pathways and responses elicited by RRV in cells which are both infected and stimulated because of neighbouring infected cells.
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spelling pubmed-98641612023-01-22 Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection Lim, Elisa X. Y. Webster, Julie A. Rudd, Penny A. Herrero, Lara J. Viruses Article Old world alphaviruses, such as Ross River virus (RRV), cause debilitating arthralgia during acute and chronic stages of the disease. RRV-induced cartilage degradation has been implicated as a cause of joint pain felt by RRV patients. Chondrocytes are a major cell type of cartilage and are involved in the production and maintenance of the cartilage matrix. It is thought that these cells may play a vital role in RRV disease pathogenesis. In this study, we used RNA-sequencing (RNA-Seq) to examine the transcriptomes of RRV-infected and bystander chondrocytes in the same environment. RRV containing green fluorescent protein (GFP) allowed for the separation of RRV-infected (GFP+) and bystander uninfected cells (GFP−). We found that whereas GFP+ and GFP− populations commonly presented similar gene expression profiles during infection, there were also unique signatures. For example, RIMS2 and FOXJ1 were unique to GFP+ cells, whilst Aim2 and CCL8 were only found in bystander chondrocytes. This indicates that careful selection of potential therapeutic targets is important to minimise adverse effects to the neighbouring uninfected cell populations. Our study serves as a resource to provide more information about the pathways and responses elicited by RRV in cells which are both infected and stimulated because of neighbouring infected cells. MDPI 2022-12-31 /pmc/articles/PMC9864161/ /pubmed/36680176 http://dx.doi.org/10.3390/v15010136 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lim, Elisa X. Y.
Webster, Julie A.
Rudd, Penny A.
Herrero, Lara J.
Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection
title Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection
title_full Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection
title_fullStr Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection
title_full_unstemmed Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection
title_short Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection
title_sort pathways activated by infected and bystander chondrocytes in response to ross river virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864161/
https://www.ncbi.nlm.nih.gov/pubmed/36680176
http://dx.doi.org/10.3390/v15010136
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