Full Skin Equivalent Models for Simulation of Burn Wound Healing, Exploring Skin Regeneration and Cytokine Response

Healing of burn injury is a complex process that often leads to the development of functional and aesthetic complications. To study skin regeneration in more detail, organotypic skin models, such as full skin equivalents (FSEs) generated from dermal matrices, can be used. Here, FSEs were generated u...

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Autores principales: Mulder, Patrick P. G., Raktoe, Rajiv S., Vlig, Marcel, Elgersma, Anouk, Middelkoop, Esther, Boekema, Bouke K. H. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864292/
https://www.ncbi.nlm.nih.gov/pubmed/36662076
http://dx.doi.org/10.3390/jfb14010029
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author Mulder, Patrick P. G.
Raktoe, Rajiv S.
Vlig, Marcel
Elgersma, Anouk
Middelkoop, Esther
Boekema, Bouke K. H. L.
author_facet Mulder, Patrick P. G.
Raktoe, Rajiv S.
Vlig, Marcel
Elgersma, Anouk
Middelkoop, Esther
Boekema, Bouke K. H. L.
author_sort Mulder, Patrick P. G.
collection PubMed
description Healing of burn injury is a complex process that often leads to the development of functional and aesthetic complications. To study skin regeneration in more detail, organotypic skin models, such as full skin equivalents (FSEs) generated from dermal matrices, can be used. Here, FSEs were generated using de-epidermalized dermis (DED) and collagen matrices MatriDerm(®) and Mucomaix(®). Our aim was to validate the MatriDerm- and Mucomaix-based FSEs for the use as in vitro models of wound healing. Therefore, we first characterized the FSEs in terms of skin development and cell proliferation. Proper dermal and epidermal morphogenesis was established in all FSEs and was comparable to ex vivo human skin models. Extension of culture time improved the organization of the epidermal layers and the basement membrane in MatriDerm-based FSE but resulted in rapid degradation of the Mucomaix-based FSE. After applying a standardized burn injury to the models, re-epithelization occurred in the DED- and MatriDerm-based FSEs at 2 weeks after injury, similar to ex vivo human skin. High levels of pro-inflammatory cytokines were present in the culture media of all models, but no significant differences were observed between models. We anticipate that these animal-free in vitro models can facilitate research on skin regeneration and can be used to test therapeutic interventions in a preclinical setting to improve wound healing.
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spelling pubmed-98642922023-01-22 Full Skin Equivalent Models for Simulation of Burn Wound Healing, Exploring Skin Regeneration and Cytokine Response Mulder, Patrick P. G. Raktoe, Rajiv S. Vlig, Marcel Elgersma, Anouk Middelkoop, Esther Boekema, Bouke K. H. L. J Funct Biomater Article Healing of burn injury is a complex process that often leads to the development of functional and aesthetic complications. To study skin regeneration in more detail, organotypic skin models, such as full skin equivalents (FSEs) generated from dermal matrices, can be used. Here, FSEs were generated using de-epidermalized dermis (DED) and collagen matrices MatriDerm(®) and Mucomaix(®). Our aim was to validate the MatriDerm- and Mucomaix-based FSEs for the use as in vitro models of wound healing. Therefore, we first characterized the FSEs in terms of skin development and cell proliferation. Proper dermal and epidermal morphogenesis was established in all FSEs and was comparable to ex vivo human skin models. Extension of culture time improved the organization of the epidermal layers and the basement membrane in MatriDerm-based FSE but resulted in rapid degradation of the Mucomaix-based FSE. After applying a standardized burn injury to the models, re-epithelization occurred in the DED- and MatriDerm-based FSEs at 2 weeks after injury, similar to ex vivo human skin. High levels of pro-inflammatory cytokines were present in the culture media of all models, but no significant differences were observed between models. We anticipate that these animal-free in vitro models can facilitate research on skin regeneration and can be used to test therapeutic interventions in a preclinical setting to improve wound healing. MDPI 2023-01-04 /pmc/articles/PMC9864292/ /pubmed/36662076 http://dx.doi.org/10.3390/jfb14010029 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mulder, Patrick P. G.
Raktoe, Rajiv S.
Vlig, Marcel
Elgersma, Anouk
Middelkoop, Esther
Boekema, Bouke K. H. L.
Full Skin Equivalent Models for Simulation of Burn Wound Healing, Exploring Skin Regeneration and Cytokine Response
title Full Skin Equivalent Models for Simulation of Burn Wound Healing, Exploring Skin Regeneration and Cytokine Response
title_full Full Skin Equivalent Models for Simulation of Burn Wound Healing, Exploring Skin Regeneration and Cytokine Response
title_fullStr Full Skin Equivalent Models for Simulation of Burn Wound Healing, Exploring Skin Regeneration and Cytokine Response
title_full_unstemmed Full Skin Equivalent Models for Simulation of Burn Wound Healing, Exploring Skin Regeneration and Cytokine Response
title_short Full Skin Equivalent Models for Simulation of Burn Wound Healing, Exploring Skin Regeneration and Cytokine Response
title_sort full skin equivalent models for simulation of burn wound healing, exploring skin regeneration and cytokine response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864292/
https://www.ncbi.nlm.nih.gov/pubmed/36662076
http://dx.doi.org/10.3390/jfb14010029
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