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An Immunomodulatory Polysaccharide–Protein Complex Isolated from the Polypore Fungus Royoporus badius

Many wild edible polypore mushrooms have medicinal value. In this study, we investigate the potential medicinal properties of the wild polypore mushroom Royoporus badius collected from north-central British Columbia, Canada. Water extract from R. badius was found to exhibit potent immunomodulatory a...

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Autores principales: Lim, Bryan C. C., Zeb, Mehreen, Li, Wai-Ming, Tang, John Z., Heiss, Christian, Tackaberry, Linda E., Massicotte, Hugues B., Egger, Keith N., Reimer, Kerry, Azadi, Parastoo, Lee, Chow H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864380/
https://www.ncbi.nlm.nih.gov/pubmed/36675908
http://dx.doi.org/10.3390/jof9010087
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author Lim, Bryan C. C.
Zeb, Mehreen
Li, Wai-Ming
Tang, John Z.
Heiss, Christian
Tackaberry, Linda E.
Massicotte, Hugues B.
Egger, Keith N.
Reimer, Kerry
Azadi, Parastoo
Lee, Chow H.
author_facet Lim, Bryan C. C.
Zeb, Mehreen
Li, Wai-Ming
Tang, John Z.
Heiss, Christian
Tackaberry, Linda E.
Massicotte, Hugues B.
Egger, Keith N.
Reimer, Kerry
Azadi, Parastoo
Lee, Chow H.
author_sort Lim, Bryan C. C.
collection PubMed
description Many wild edible polypore mushrooms have medicinal value. In this study, we investigate the potential medicinal properties of the wild polypore mushroom Royoporus badius collected from north-central British Columbia, Canada. Water extract from R. badius was found to exhibit potent immunomodulatory activity. The extract was purified using DEAE-Sephadex anion-exchange chromatography as well as Sephacryl S-500 and HPLC BioSEC5 size-exclusion chromatography, to yield a novel polysaccharide-protein complex (IMPP-Rb).IMPP-Rb has a peak maxima molecular weight (M(p)) of 950 kDa. GC-MS analyses showed that IMPP-Rb is composed predominantly of glucose (49.2%), galactose (11.3%), mannose (10.8%), rhamnose (9.6%), and galacturonic acid (8.2%), with smaller amounts of xylose (5.2%), fucose (2.8%), N-acetyl glucosamine (1.8%), and arabinose (1.2%). IMPP-Rb has multiple linkages, with 4-Glcp, 4-Manp, 6-Manp, 3,4-Manp, 4-Xylp, and 2-Rhap being the most prominent. IMPP-Rb is capable of inducing many cytokines in vitro and the protein component is indispensable for its immunomodulatory activity. IMPP-Rb has potential application as an immuno-stimulatory agent with pharmaceutical value.
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spelling pubmed-98643802023-01-22 An Immunomodulatory Polysaccharide–Protein Complex Isolated from the Polypore Fungus Royoporus badius Lim, Bryan C. C. Zeb, Mehreen Li, Wai-Ming Tang, John Z. Heiss, Christian Tackaberry, Linda E. Massicotte, Hugues B. Egger, Keith N. Reimer, Kerry Azadi, Parastoo Lee, Chow H. J Fungi (Basel) Article Many wild edible polypore mushrooms have medicinal value. In this study, we investigate the potential medicinal properties of the wild polypore mushroom Royoporus badius collected from north-central British Columbia, Canada. Water extract from R. badius was found to exhibit potent immunomodulatory activity. The extract was purified using DEAE-Sephadex anion-exchange chromatography as well as Sephacryl S-500 and HPLC BioSEC5 size-exclusion chromatography, to yield a novel polysaccharide-protein complex (IMPP-Rb).IMPP-Rb has a peak maxima molecular weight (M(p)) of 950 kDa. GC-MS analyses showed that IMPP-Rb is composed predominantly of glucose (49.2%), galactose (11.3%), mannose (10.8%), rhamnose (9.6%), and galacturonic acid (8.2%), with smaller amounts of xylose (5.2%), fucose (2.8%), N-acetyl glucosamine (1.8%), and arabinose (1.2%). IMPP-Rb has multiple linkages, with 4-Glcp, 4-Manp, 6-Manp, 3,4-Manp, 4-Xylp, and 2-Rhap being the most prominent. IMPP-Rb is capable of inducing many cytokines in vitro and the protein component is indispensable for its immunomodulatory activity. IMPP-Rb has potential application as an immuno-stimulatory agent with pharmaceutical value. MDPI 2023-01-06 /pmc/articles/PMC9864380/ /pubmed/36675908 http://dx.doi.org/10.3390/jof9010087 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lim, Bryan C. C.
Zeb, Mehreen
Li, Wai-Ming
Tang, John Z.
Heiss, Christian
Tackaberry, Linda E.
Massicotte, Hugues B.
Egger, Keith N.
Reimer, Kerry
Azadi, Parastoo
Lee, Chow H.
An Immunomodulatory Polysaccharide–Protein Complex Isolated from the Polypore Fungus Royoporus badius
title An Immunomodulatory Polysaccharide–Protein Complex Isolated from the Polypore Fungus Royoporus badius
title_full An Immunomodulatory Polysaccharide–Protein Complex Isolated from the Polypore Fungus Royoporus badius
title_fullStr An Immunomodulatory Polysaccharide–Protein Complex Isolated from the Polypore Fungus Royoporus badius
title_full_unstemmed An Immunomodulatory Polysaccharide–Protein Complex Isolated from the Polypore Fungus Royoporus badius
title_short An Immunomodulatory Polysaccharide–Protein Complex Isolated from the Polypore Fungus Royoporus badius
title_sort immunomodulatory polysaccharide–protein complex isolated from the polypore fungus royoporus badius
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864380/
https://www.ncbi.nlm.nih.gov/pubmed/36675908
http://dx.doi.org/10.3390/jof9010087
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