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Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells

The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the struct...

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Autores principales: Colunga-Saucedo, Mayra, Rubio-Hernandez, Edson I., Coronado-Ipiña, Miguel A., Rosales-Mendoza, Sergio, Castillo, Claudia G., Comas-Garcia, Mauricio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864538/
https://www.ncbi.nlm.nih.gov/pubmed/36680173
http://dx.doi.org/10.3390/v15010132
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author Colunga-Saucedo, Mayra
Rubio-Hernandez, Edson I.
Coronado-Ipiña, Miguel A.
Rosales-Mendoza, Sergio
Castillo, Claudia G.
Comas-Garcia, Mauricio
author_facet Colunga-Saucedo, Mayra
Rubio-Hernandez, Edson I.
Coronado-Ipiña, Miguel A.
Rosales-Mendoza, Sergio
Castillo, Claudia G.
Comas-Garcia, Mauricio
author_sort Colunga-Saucedo, Mayra
collection PubMed
description The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells.
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spelling pubmed-98645382023-01-22 Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells Colunga-Saucedo, Mayra Rubio-Hernandez, Edson I. Coronado-Ipiña, Miguel A. Rosales-Mendoza, Sergio Castillo, Claudia G. Comas-Garcia, Mauricio Viruses Article The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells. MDPI 2022-12-31 /pmc/articles/PMC9864538/ /pubmed/36680173 http://dx.doi.org/10.3390/v15010132 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Colunga-Saucedo, Mayra
Rubio-Hernandez, Edson I.
Coronado-Ipiña, Miguel A.
Rosales-Mendoza, Sergio
Castillo, Claudia G.
Comas-Garcia, Mauricio
Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
title Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
title_full Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
title_fullStr Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
title_full_unstemmed Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
title_short Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
title_sort construction of a chikungunya virus, replicon, and helper plasmids for transfection of mammalian cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864538/
https://www.ncbi.nlm.nih.gov/pubmed/36680173
http://dx.doi.org/10.3390/v15010132
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