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Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the struct...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864538/ https://www.ncbi.nlm.nih.gov/pubmed/36680173 http://dx.doi.org/10.3390/v15010132 |
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author | Colunga-Saucedo, Mayra Rubio-Hernandez, Edson I. Coronado-Ipiña, Miguel A. Rosales-Mendoza, Sergio Castillo, Claudia G. Comas-Garcia, Mauricio |
author_facet | Colunga-Saucedo, Mayra Rubio-Hernandez, Edson I. Coronado-Ipiña, Miguel A. Rosales-Mendoza, Sergio Castillo, Claudia G. Comas-Garcia, Mauricio |
author_sort | Colunga-Saucedo, Mayra |
collection | PubMed |
description | The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells. |
format | Online Article Text |
id | pubmed-9864538 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98645382023-01-22 Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells Colunga-Saucedo, Mayra Rubio-Hernandez, Edson I. Coronado-Ipiña, Miguel A. Rosales-Mendoza, Sergio Castillo, Claudia G. Comas-Garcia, Mauricio Viruses Article The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells. MDPI 2022-12-31 /pmc/articles/PMC9864538/ /pubmed/36680173 http://dx.doi.org/10.3390/v15010132 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Colunga-Saucedo, Mayra Rubio-Hernandez, Edson I. Coronado-Ipiña, Miguel A. Rosales-Mendoza, Sergio Castillo, Claudia G. Comas-Garcia, Mauricio Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells |
title | Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells |
title_full | Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells |
title_fullStr | Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells |
title_full_unstemmed | Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells |
title_short | Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells |
title_sort | construction of a chikungunya virus, replicon, and helper plasmids for transfection of mammalian cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864538/ https://www.ncbi.nlm.nih.gov/pubmed/36680173 http://dx.doi.org/10.3390/v15010132 |
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