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Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks

The ectoparasite Ixodes ricinus is an important vector for many tick-borne diseases (TBD) in the northern hemisphere, such as Lyme borreliosis, rickettsiosis, human granulocytic anaplasmosis, or tick-borne encephalitis virus. As climate change will lead to rising temperatures in the next years, we e...

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Autores principales: Wiesinger, Anna, Wenderlein, Jasmin, Ulrich, Sebastian, Hiereth, Stephanie, Chitimia-Dobler, Lidia, Straubinger, Reinhard K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864629/
https://www.ncbi.nlm.nih.gov/pubmed/36674613
http://dx.doi.org/10.3390/ijms24021100
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author Wiesinger, Anna
Wenderlein, Jasmin
Ulrich, Sebastian
Hiereth, Stephanie
Chitimia-Dobler, Lidia
Straubinger, Reinhard K.
author_facet Wiesinger, Anna
Wenderlein, Jasmin
Ulrich, Sebastian
Hiereth, Stephanie
Chitimia-Dobler, Lidia
Straubinger, Reinhard K.
author_sort Wiesinger, Anna
collection PubMed
description The ectoparasite Ixodes ricinus is an important vector for many tick-borne diseases (TBD) in the northern hemisphere, such as Lyme borreliosis, rickettsiosis, human granulocytic anaplasmosis, or tick-borne encephalitis virus. As climate change will lead to rising temperatures in the next years, we expect an increase in tick activity, tick population, and thus in the spread of TBD. Consequently, it has never been more critical to understand relationships within the microbial communities in ticks that might contribute to the tick’s fitness and the occurrence of TBD. Therefore, we analyzed the microbiota in different tick tissues such as midgut, salivary glands, and residual tick material, as well as the microbiota in complete Ixodes ricinus ticks using 16S rRNA gene amplicon sequencing. By using a newly developed DNA extraction protocol for tick tissue samples and a self-designed mock community, we were able to detect endosymbionts and pathogens that have been described in the literature previously. Further, this study displayed the usefulness of including a mock community during bioinformatic analysis to identify essential bacteria within the tick.
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spelling pubmed-98646292023-01-22 Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks Wiesinger, Anna Wenderlein, Jasmin Ulrich, Sebastian Hiereth, Stephanie Chitimia-Dobler, Lidia Straubinger, Reinhard K. Int J Mol Sci Article The ectoparasite Ixodes ricinus is an important vector for many tick-borne diseases (TBD) in the northern hemisphere, such as Lyme borreliosis, rickettsiosis, human granulocytic anaplasmosis, or tick-borne encephalitis virus. As climate change will lead to rising temperatures in the next years, we expect an increase in tick activity, tick population, and thus in the spread of TBD. Consequently, it has never been more critical to understand relationships within the microbial communities in ticks that might contribute to the tick’s fitness and the occurrence of TBD. Therefore, we analyzed the microbiota in different tick tissues such as midgut, salivary glands, and residual tick material, as well as the microbiota in complete Ixodes ricinus ticks using 16S rRNA gene amplicon sequencing. By using a newly developed DNA extraction protocol for tick tissue samples and a self-designed mock community, we were able to detect endosymbionts and pathogens that have been described in the literature previously. Further, this study displayed the usefulness of including a mock community during bioinformatic analysis to identify essential bacteria within the tick. MDPI 2023-01-06 /pmc/articles/PMC9864629/ /pubmed/36674613 http://dx.doi.org/10.3390/ijms24021100 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wiesinger, Anna
Wenderlein, Jasmin
Ulrich, Sebastian
Hiereth, Stephanie
Chitimia-Dobler, Lidia
Straubinger, Reinhard K.
Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks
title Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks
title_full Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks
title_fullStr Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks
title_full_unstemmed Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks
title_short Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks
title_sort revealing the tick microbiome: insights into midgut and salivary gland microbiota of female ixodes ricinus ticks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864629/
https://www.ncbi.nlm.nih.gov/pubmed/36674613
http://dx.doi.org/10.3390/ijms24021100
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