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Biomaterial Inks from Peptide-Functionalized Silk Fibers for 3D Printing of Futuristic Wound-Healing and Sensing Materials

This study illustrates the sensing and wound healing properties of silk fibroin in combination with peptide patterns, with an emphasis on the printability of multilayered grids, and envisions possible applications of these next-generation silk-based materials. Functionalized silk fibers covalently l...

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Autores principales: Ceccarini, Maria Rachele, Palazzi, Valentina, Salvati, Raffaele, Chiesa, Irene, De Maria, Carmelo, Bonafoni, Stefania, Mezzanotte, Paolo, Codini, Michela, Pacini, Lorenzo, Errante, Fosca, Rovero, Paolo, Morabito, Antonino, Beccari, Tommaso, Roselli, Luca, Valentini, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864705/
https://www.ncbi.nlm.nih.gov/pubmed/36674467
http://dx.doi.org/10.3390/ijms24020947
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author Ceccarini, Maria Rachele
Palazzi, Valentina
Salvati, Raffaele
Chiesa, Irene
De Maria, Carmelo
Bonafoni, Stefania
Mezzanotte, Paolo
Codini, Michela
Pacini, Lorenzo
Errante, Fosca
Rovero, Paolo
Morabito, Antonino
Beccari, Tommaso
Roselli, Luca
Valentini, Luca
author_facet Ceccarini, Maria Rachele
Palazzi, Valentina
Salvati, Raffaele
Chiesa, Irene
De Maria, Carmelo
Bonafoni, Stefania
Mezzanotte, Paolo
Codini, Michela
Pacini, Lorenzo
Errante, Fosca
Rovero, Paolo
Morabito, Antonino
Beccari, Tommaso
Roselli, Luca
Valentini, Luca
author_sort Ceccarini, Maria Rachele
collection PubMed
description This study illustrates the sensing and wound healing properties of silk fibroin in combination with peptide patterns, with an emphasis on the printability of multilayered grids, and envisions possible applications of these next-generation silk-based materials. Functionalized silk fibers covalently linked to an arginine–glycine–aspartic acid (RGD) peptide create a platform for preparing a biomaterial ink for 3D printing of grid-like piezoresistors with wound-healing and sensing properties. The culture medium obtained from 3D-printed silk fibroin enriched with RGD peptide improves cell adhesion, accelerating skin repair. Specifically, RGD peptide-modified silk fibroin demonstrated biocompatibility, enhanced cell adhesion, and higher wound closure rates at lower concentration than the neat peptide. It was also shown that the printing of peptide-modified silk fibroin produces a piezoresistive transducer that is the active component of a sensor based on a Schottky diode harmonic transponder encoding information about pressure. We discovered that such biomaterial ink printed in a multilayered grid can be used as a humidity sensor. Furthermore, humidity activates a transition between low and high conductivity states in this medium that is retained unless a negative voltage is applied, paving the way for utilization in non-volatile organic memory devices. Globally, these results pave the way for promising applications, such as monitoring parameters such as human wound care and being integrated in bio-implantable processors.
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spelling pubmed-98647052023-01-22 Biomaterial Inks from Peptide-Functionalized Silk Fibers for 3D Printing of Futuristic Wound-Healing and Sensing Materials Ceccarini, Maria Rachele Palazzi, Valentina Salvati, Raffaele Chiesa, Irene De Maria, Carmelo Bonafoni, Stefania Mezzanotte, Paolo Codini, Michela Pacini, Lorenzo Errante, Fosca Rovero, Paolo Morabito, Antonino Beccari, Tommaso Roselli, Luca Valentini, Luca Int J Mol Sci Article This study illustrates the sensing and wound healing properties of silk fibroin in combination with peptide patterns, with an emphasis on the printability of multilayered grids, and envisions possible applications of these next-generation silk-based materials. Functionalized silk fibers covalently linked to an arginine–glycine–aspartic acid (RGD) peptide create a platform for preparing a biomaterial ink for 3D printing of grid-like piezoresistors with wound-healing and sensing properties. The culture medium obtained from 3D-printed silk fibroin enriched with RGD peptide improves cell adhesion, accelerating skin repair. Specifically, RGD peptide-modified silk fibroin demonstrated biocompatibility, enhanced cell adhesion, and higher wound closure rates at lower concentration than the neat peptide. It was also shown that the printing of peptide-modified silk fibroin produces a piezoresistive transducer that is the active component of a sensor based on a Schottky diode harmonic transponder encoding information about pressure. We discovered that such biomaterial ink printed in a multilayered grid can be used as a humidity sensor. Furthermore, humidity activates a transition between low and high conductivity states in this medium that is retained unless a negative voltage is applied, paving the way for utilization in non-volatile organic memory devices. Globally, these results pave the way for promising applications, such as monitoring parameters such as human wound care and being integrated in bio-implantable processors. MDPI 2023-01-04 /pmc/articles/PMC9864705/ /pubmed/36674467 http://dx.doi.org/10.3390/ijms24020947 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ceccarini, Maria Rachele
Palazzi, Valentina
Salvati, Raffaele
Chiesa, Irene
De Maria, Carmelo
Bonafoni, Stefania
Mezzanotte, Paolo
Codini, Michela
Pacini, Lorenzo
Errante, Fosca
Rovero, Paolo
Morabito, Antonino
Beccari, Tommaso
Roselli, Luca
Valentini, Luca
Biomaterial Inks from Peptide-Functionalized Silk Fibers for 3D Printing of Futuristic Wound-Healing and Sensing Materials
title Biomaterial Inks from Peptide-Functionalized Silk Fibers for 3D Printing of Futuristic Wound-Healing and Sensing Materials
title_full Biomaterial Inks from Peptide-Functionalized Silk Fibers for 3D Printing of Futuristic Wound-Healing and Sensing Materials
title_fullStr Biomaterial Inks from Peptide-Functionalized Silk Fibers for 3D Printing of Futuristic Wound-Healing and Sensing Materials
title_full_unstemmed Biomaterial Inks from Peptide-Functionalized Silk Fibers for 3D Printing of Futuristic Wound-Healing and Sensing Materials
title_short Biomaterial Inks from Peptide-Functionalized Silk Fibers for 3D Printing of Futuristic Wound-Healing and Sensing Materials
title_sort biomaterial inks from peptide-functionalized silk fibers for 3d printing of futuristic wound-healing and sensing materials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864705/
https://www.ncbi.nlm.nih.gov/pubmed/36674467
http://dx.doi.org/10.3390/ijms24020947
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