Cargando…

Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs

Synthetic poly(amino acids) are a unique class of macromolecules imitating natural polypeptides and are widely considered as carriers for drug and gene delivery. In this work, we synthesized, characterized and studied the properties of amphiphilic copolymers obtained by the post-polymerization modif...

Descripción completa

Detalles Bibliográficos
Autores principales: Dzhuzha, Apollinariia Yu., Tarasenko, Irina I., Atanase, Leonard Ionut, Lavrentieva, Antonina, Korzhikova-Vlakh, Evgenia G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864831/
https://www.ncbi.nlm.nih.gov/pubmed/36674566
http://dx.doi.org/10.3390/ijms24021049
_version_ 1784875682382217216
author Dzhuzha, Apollinariia Yu.
Tarasenko, Irina I.
Atanase, Leonard Ionut
Lavrentieva, Antonina
Korzhikova-Vlakh, Evgenia G.
author_facet Dzhuzha, Apollinariia Yu.
Tarasenko, Irina I.
Atanase, Leonard Ionut
Lavrentieva, Antonina
Korzhikova-Vlakh, Evgenia G.
author_sort Dzhuzha, Apollinariia Yu.
collection PubMed
description Synthetic poly(amino acids) are a unique class of macromolecules imitating natural polypeptides and are widely considered as carriers for drug and gene delivery. In this work, we synthesized, characterized and studied the properties of amphiphilic copolymers obtained by the post-polymerization modification of poly(α,L-glutamic acid) with various hydrophobic and basic L-amino acids and D-glucosamine. The resulting glycopolypeptides were capable of forming nanoparticles that exhibited reduced macrophage uptake and were non-toxic to human lung epithelial cells (BEAS-2B). Moreover, the developed nanoparticles were suitable for loading hydrophobic cargo. In particular, paclitaxel nanoformulations had a size of 170–330 nm and demonstrated a high cytostatic efficacy against human lung adenocarcinoma (A549). In general, the obtained nanoparticles were comparable in terms of their characteristics and properties to those based on amphiphilic (glyco)polypeptides obtained by copolymerization methods.
format Online
Article
Text
id pubmed-9864831
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98648312023-01-22 Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs Dzhuzha, Apollinariia Yu. Tarasenko, Irina I. Atanase, Leonard Ionut Lavrentieva, Antonina Korzhikova-Vlakh, Evgenia G. Int J Mol Sci Article Synthetic poly(amino acids) are a unique class of macromolecules imitating natural polypeptides and are widely considered as carriers for drug and gene delivery. In this work, we synthesized, characterized and studied the properties of amphiphilic copolymers obtained by the post-polymerization modification of poly(α,L-glutamic acid) with various hydrophobic and basic L-amino acids and D-glucosamine. The resulting glycopolypeptides were capable of forming nanoparticles that exhibited reduced macrophage uptake and were non-toxic to human lung epithelial cells (BEAS-2B). Moreover, the developed nanoparticles were suitable for loading hydrophobic cargo. In particular, paclitaxel nanoformulations had a size of 170–330 nm and demonstrated a high cytostatic efficacy against human lung adenocarcinoma (A549). In general, the obtained nanoparticles were comparable in terms of their characteristics and properties to those based on amphiphilic (glyco)polypeptides obtained by copolymerization methods. MDPI 2023-01-05 /pmc/articles/PMC9864831/ /pubmed/36674566 http://dx.doi.org/10.3390/ijms24021049 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dzhuzha, Apollinariia Yu.
Tarasenko, Irina I.
Atanase, Leonard Ionut
Lavrentieva, Antonina
Korzhikova-Vlakh, Evgenia G.
Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs
title Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs
title_full Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs
title_fullStr Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs
title_full_unstemmed Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs
title_short Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs
title_sort amphiphilic polypeptides obtained by the post-polymerization modification of poly(glutamic acid) and their evaluation as delivery systems for hydrophobic drugs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864831/
https://www.ncbi.nlm.nih.gov/pubmed/36674566
http://dx.doi.org/10.3390/ijms24021049
work_keys_str_mv AT dzhuzhaapollinariiayu amphiphilicpolypeptidesobtainedbythepostpolymerizationmodificationofpolyglutamicacidandtheirevaluationasdeliverysystemsforhydrophobicdrugs
AT tarasenkoirinai amphiphilicpolypeptidesobtainedbythepostpolymerizationmodificationofpolyglutamicacidandtheirevaluationasdeliverysystemsforhydrophobicdrugs
AT atanaseleonardionut amphiphilicpolypeptidesobtainedbythepostpolymerizationmodificationofpolyglutamicacidandtheirevaluationasdeliverysystemsforhydrophobicdrugs
AT lavrentievaantonina amphiphilicpolypeptidesobtainedbythepostpolymerizationmodificationofpolyglutamicacidandtheirevaluationasdeliverysystemsforhydrophobicdrugs
AT korzhikovavlakhevgeniag amphiphilicpolypeptidesobtainedbythepostpolymerizationmodificationofpolyglutamicacidandtheirevaluationasdeliverysystemsforhydrophobicdrugs