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Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability

Monkeypox is an infectious zoonotic disease caused by an Orthopoxvirus and results in symptoms similar to smallpox. In a recent outbreak, monkeypox virus (MPXV) cases have been reported globally since May 2022, and the numbers are increasing. Monkeypox was first diagnosed in humans in the Democratic...

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Autores principales: Yadav, Priya, Devasurmutt, Yashas, Tatu, Utpal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864997/
https://www.ncbi.nlm.nih.gov/pubmed/36680170
http://dx.doi.org/10.3390/v15010127
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author Yadav, Priya
Devasurmutt, Yashas
Tatu, Utpal
author_facet Yadav, Priya
Devasurmutt, Yashas
Tatu, Utpal
author_sort Yadav, Priya
collection PubMed
description Monkeypox is an infectious zoonotic disease caused by an Orthopoxvirus and results in symptoms similar to smallpox. In a recent outbreak, monkeypox virus (MPXV) cases have been reported globally since May 2022, and the numbers are increasing. Monkeypox was first diagnosed in humans in the Democratic Republic of Congo and has now spread to throughout Europe, the USA, and Africa. In this study, we analyzed the whole genome sequences of MPXV sequences from recent outbreaks in various countries and performed phylogenomic analysis. Our analysis of the available human MPXV strains showed the highest mutations per sample in 2022 with the average number of mutations per sample being the highest in South America and the European continents in 2022. We analyzed specific mutations in 11 Indian MPXV strains occurring in the variable end regions of the MPXV genome, where the mutation number was as high as 10 mutations per gene. Among these, envelope glycoproteins, the B2R protein, the Ankyrin repeat protein, DNA polymerase, and the INF alpha receptor-like secreted glycoprotein were seen to have a relatively high number of mutations. We discussed the stabilizing effects of the mutations in some of the highly mutating proteins. Our results showed that the proteins involved in binding to the host receptors were mutating at a faster rate, which empowered the virus for active selection towards increased disease transmissibility and severity.
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spelling pubmed-98649972023-01-22 Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability Yadav, Priya Devasurmutt, Yashas Tatu, Utpal Viruses Article Monkeypox is an infectious zoonotic disease caused by an Orthopoxvirus and results in symptoms similar to smallpox. In a recent outbreak, monkeypox virus (MPXV) cases have been reported globally since May 2022, and the numbers are increasing. Monkeypox was first diagnosed in humans in the Democratic Republic of Congo and has now spread to throughout Europe, the USA, and Africa. In this study, we analyzed the whole genome sequences of MPXV sequences from recent outbreaks in various countries and performed phylogenomic analysis. Our analysis of the available human MPXV strains showed the highest mutations per sample in 2022 with the average number of mutations per sample being the highest in South America and the European continents in 2022. We analyzed specific mutations in 11 Indian MPXV strains occurring in the variable end regions of the MPXV genome, where the mutation number was as high as 10 mutations per gene. Among these, envelope glycoproteins, the B2R protein, the Ankyrin repeat protein, DNA polymerase, and the INF alpha receptor-like secreted glycoprotein were seen to have a relatively high number of mutations. We discussed the stabilizing effects of the mutations in some of the highly mutating proteins. Our results showed that the proteins involved in binding to the host receptors were mutating at a faster rate, which empowered the virus for active selection towards increased disease transmissibility and severity. MDPI 2022-12-31 /pmc/articles/PMC9864997/ /pubmed/36680170 http://dx.doi.org/10.3390/v15010127 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yadav, Priya
Devasurmutt, Yashas
Tatu, Utpal
Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability
title Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability
title_full Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability
title_fullStr Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability
title_full_unstemmed Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability
title_short Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability
title_sort phylogenomic and structural analysis of the monkeypox virus shows evolution towards increased stability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9864997/
https://www.ncbi.nlm.nih.gov/pubmed/36680170
http://dx.doi.org/10.3390/v15010127
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