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Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor

Trypanosoma cruzi is the etiologic agent of Chagas disease, a parasitic disease of great medical importance on the American continent. Trypomastigote infection’s initial step in a mammalian host is vital for the parasite’s life cycle. A trypomastigote’s surface presents many molecules, some of which...

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Autores principales: Espinoza, Bertha, Martínez, Ignacio, Martínez-Velasco, María Luisa, Rodríguez-Sosa, Miriam, González-Canto, Augusto, Vázquez-Mendoza, Alicia, Terrazas, Luis I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865002/
https://www.ncbi.nlm.nih.gov/pubmed/36678452
http://dx.doi.org/10.3390/pathogens12010105
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author Espinoza, Bertha
Martínez, Ignacio
Martínez-Velasco, María Luisa
Rodríguez-Sosa, Miriam
González-Canto, Augusto
Vázquez-Mendoza, Alicia
Terrazas, Luis I.
author_facet Espinoza, Bertha
Martínez, Ignacio
Martínez-Velasco, María Luisa
Rodríguez-Sosa, Miriam
González-Canto, Augusto
Vázquez-Mendoza, Alicia
Terrazas, Luis I.
author_sort Espinoza, Bertha
collection PubMed
description Trypanosoma cruzi is the etiologic agent of Chagas disease, a parasitic disease of great medical importance on the American continent. Trypomastigote infection’s initial step in a mammalian host is vital for the parasite’s life cycle. A trypomastigote’s surface presents many molecules, some of which have been proposed to be involved in the infection process, including a glycoprotein family called mucin-associated surface proteins (MASPs). This work describes a 49-kDa molecule (MASP49) that belongs to this family and is expressed mainly on the surfaces of amastigotes and trypomastigotes but can be found in extracts and the membrane-enriched fractions of epimastigotes. This protein is partially GPI-anchored to the surface and has a role during the internalization process, since its blockade with specific antibodies decreases parasite entry into Vero cells by 62%. This work shows that MASP49 binds to peritoneal macrophages and rat cardiomyocytes, undergoes glycosylation via galactose N-acetylgalactosamine, and can attach to the macrophage murine C-type lectin receptor (mMGL). These results suggest that MASP49 can be considered a virulence factor in T. cruzi, and a better understanding of its role in the infection process is necessary.
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spelling pubmed-98650022023-01-22 Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor Espinoza, Bertha Martínez, Ignacio Martínez-Velasco, María Luisa Rodríguez-Sosa, Miriam González-Canto, Augusto Vázquez-Mendoza, Alicia Terrazas, Luis I. Pathogens Article Trypanosoma cruzi is the etiologic agent of Chagas disease, a parasitic disease of great medical importance on the American continent. Trypomastigote infection’s initial step in a mammalian host is vital for the parasite’s life cycle. A trypomastigote’s surface presents many molecules, some of which have been proposed to be involved in the infection process, including a glycoprotein family called mucin-associated surface proteins (MASPs). This work describes a 49-kDa molecule (MASP49) that belongs to this family and is expressed mainly on the surfaces of amastigotes and trypomastigotes but can be found in extracts and the membrane-enriched fractions of epimastigotes. This protein is partially GPI-anchored to the surface and has a role during the internalization process, since its blockade with specific antibodies decreases parasite entry into Vero cells by 62%. This work shows that MASP49 binds to peritoneal macrophages and rat cardiomyocytes, undergoes glycosylation via galactose N-acetylgalactosamine, and can attach to the macrophage murine C-type lectin receptor (mMGL). These results suggest that MASP49 can be considered a virulence factor in T. cruzi, and a better understanding of its role in the infection process is necessary. MDPI 2023-01-07 /pmc/articles/PMC9865002/ /pubmed/36678452 http://dx.doi.org/10.3390/pathogens12010105 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Espinoza, Bertha
Martínez, Ignacio
Martínez-Velasco, María Luisa
Rodríguez-Sosa, Miriam
González-Canto, Augusto
Vázquez-Mendoza, Alicia
Terrazas, Luis I.
Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor
title Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor
title_full Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor
title_fullStr Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor
title_full_unstemmed Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor
title_short Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor
title_sort role of a 49 kda trypanosoma cruzi mucin-associated surface protein (masp49) during the infection process and identification of a mammalian cell surface receptor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865002/
https://www.ncbi.nlm.nih.gov/pubmed/36678452
http://dx.doi.org/10.3390/pathogens12010105
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