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Transcriptome Profile of Fusarium graminearum Treated by Putrescine
Fusarium graminearum (F. graminearum) is the main pathogen of Fusarium head blight (FHB) in wheat, barley, and corn. Deoxynivalenol (DON), produced by F. graminearum, is the most prevalent toxin associated with FHB. The wheat defense compound putrescine can promote DON production during F. graminear...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865016/ https://www.ncbi.nlm.nih.gov/pubmed/36675881 http://dx.doi.org/10.3390/jof9010060 |
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author | Zhang, Lina Zhou, Xishi Li, Pengfeng Wang, Yiwei Hu, Qianyong Shang, Yuping Chen, Yunshen Zhu, Xiying Feng, Hongjie Zhang, Cuijun |
author_facet | Zhang, Lina Zhou, Xishi Li, Pengfeng Wang, Yiwei Hu, Qianyong Shang, Yuping Chen, Yunshen Zhu, Xiying Feng, Hongjie Zhang, Cuijun |
author_sort | Zhang, Lina |
collection | PubMed |
description | Fusarium graminearum (F. graminearum) is the main pathogen of Fusarium head blight (FHB) in wheat, barley, and corn. Deoxynivalenol (DON), produced by F. graminearum, is the most prevalent toxin associated with FHB. The wheat defense compound putrescine can promote DON production during F. graminearum infection. However, the underlying mechanisms of putrescine-induced DON synthesis are not well-studied. To investigate the effect of putrescine on the global transcriptional regulation of F. graminearum, we treated F. graminearum with putrescine and performed RNA deep sequencing. We found that putrescine can largely affect the transcriptome of F. graminearum. Gene ontology (GO) and KEGG enrichment analysis revealed that having a large amount of DEGs was associated with ribosome biogenesis, carboxylic acid metabolism, glycolysis/gluconeogenesis, and amino acid metabolism pathways. Co-expression analysis showed that 327 genes had similar expression patterns to FgTRI genes and were assigned to the same module. In addition, three transcription factor genes were identified as hub genes in this module, indicating that they may play important roles in DON synthesis. These results provide important clues for further analysis of the molecular mechanisms of putrescine-induced DON synthesis and will facilitate the study of the pathogenic mechanisms of FHB. |
format | Online Article Text |
id | pubmed-9865016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98650162023-01-22 Transcriptome Profile of Fusarium graminearum Treated by Putrescine Zhang, Lina Zhou, Xishi Li, Pengfeng Wang, Yiwei Hu, Qianyong Shang, Yuping Chen, Yunshen Zhu, Xiying Feng, Hongjie Zhang, Cuijun J Fungi (Basel) Article Fusarium graminearum (F. graminearum) is the main pathogen of Fusarium head blight (FHB) in wheat, barley, and corn. Deoxynivalenol (DON), produced by F. graminearum, is the most prevalent toxin associated with FHB. The wheat defense compound putrescine can promote DON production during F. graminearum infection. However, the underlying mechanisms of putrescine-induced DON synthesis are not well-studied. To investigate the effect of putrescine on the global transcriptional regulation of F. graminearum, we treated F. graminearum with putrescine and performed RNA deep sequencing. We found that putrescine can largely affect the transcriptome of F. graminearum. Gene ontology (GO) and KEGG enrichment analysis revealed that having a large amount of DEGs was associated with ribosome biogenesis, carboxylic acid metabolism, glycolysis/gluconeogenesis, and amino acid metabolism pathways. Co-expression analysis showed that 327 genes had similar expression patterns to FgTRI genes and were assigned to the same module. In addition, three transcription factor genes were identified as hub genes in this module, indicating that they may play important roles in DON synthesis. These results provide important clues for further analysis of the molecular mechanisms of putrescine-induced DON synthesis and will facilitate the study of the pathogenic mechanisms of FHB. MDPI 2022-12-30 /pmc/articles/PMC9865016/ /pubmed/36675881 http://dx.doi.org/10.3390/jof9010060 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Lina Zhou, Xishi Li, Pengfeng Wang, Yiwei Hu, Qianyong Shang, Yuping Chen, Yunshen Zhu, Xiying Feng, Hongjie Zhang, Cuijun Transcriptome Profile of Fusarium graminearum Treated by Putrescine |
title | Transcriptome Profile of Fusarium graminearum Treated by Putrescine |
title_full | Transcriptome Profile of Fusarium graminearum Treated by Putrescine |
title_fullStr | Transcriptome Profile of Fusarium graminearum Treated by Putrescine |
title_full_unstemmed | Transcriptome Profile of Fusarium graminearum Treated by Putrescine |
title_short | Transcriptome Profile of Fusarium graminearum Treated by Putrescine |
title_sort | transcriptome profile of fusarium graminearum treated by putrescine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865016/ https://www.ncbi.nlm.nih.gov/pubmed/36675881 http://dx.doi.org/10.3390/jof9010060 |
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