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Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes
Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865253/ https://www.ncbi.nlm.nih.gov/pubmed/36677111 http://dx.doi.org/10.3390/mi14010051 |
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author | Slotvitsky, Mikhail Berezhnoy, Andrey Scherbina, Serafima Rimskaya, Beatrisa Tsvelaya, Valerya Balashov, Victor Efimov, Anton E. Agapov, Igor Agladze, Konstantin |
author_facet | Slotvitsky, Mikhail Berezhnoy, Andrey Scherbina, Serafima Rimskaya, Beatrisa Tsvelaya, Valerya Balashov, Victor Efimov, Anton E. Agapov, Igor Agladze, Konstantin |
author_sort | Slotvitsky, Mikhail |
collection | PubMed |
description | Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of the cardiomyocytes has substantial challenges related to cell survival and electrophysiological coupling with recipient tissue. Current methods of manipulating cell suspensions do not allow one to control the processes of adhesion of injected cells to the tissue and electrophysiological coupling with surrounding cells. In this article, we documented the possibility of influencing these processes using polymer kernels: biocompatible fiber fragments of subcellular size that can be adsorbed to a cell, thereby creating the minimum necessary adhesion foci to shape the cell and provide support for the organization of the cytoskeleton and the contractile apparatus prior to adhesion to the recipient tissue. Using optical excitation markers, the restoration of the excitability of cardiomyocytes in suspension upon adsorption of polymer kernels was shown. It increased the likelihood of the formation of a stable electrophysiological coupling in vitro. The obtained results may be considered as a proof of concept that the stochastic engraftment process of injected suspension cells can be controlled by smart biomaterials. |
format | Online Article Text |
id | pubmed-9865253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98652532023-01-22 Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes Slotvitsky, Mikhail Berezhnoy, Andrey Scherbina, Serafima Rimskaya, Beatrisa Tsvelaya, Valerya Balashov, Victor Efimov, Anton E. Agapov, Igor Agladze, Konstantin Micromachines (Basel) Article Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of the cardiomyocytes has substantial challenges related to cell survival and electrophysiological coupling with recipient tissue. Current methods of manipulating cell suspensions do not allow one to control the processes of adhesion of injected cells to the tissue and electrophysiological coupling with surrounding cells. In this article, we documented the possibility of influencing these processes using polymer kernels: biocompatible fiber fragments of subcellular size that can be adsorbed to a cell, thereby creating the minimum necessary adhesion foci to shape the cell and provide support for the organization of the cytoskeleton and the contractile apparatus prior to adhesion to the recipient tissue. Using optical excitation markers, the restoration of the excitability of cardiomyocytes in suspension upon adsorption of polymer kernels was shown. It increased the likelihood of the formation of a stable electrophysiological coupling in vitro. The obtained results may be considered as a proof of concept that the stochastic engraftment process of injected suspension cells can be controlled by smart biomaterials. MDPI 2022-12-25 /pmc/articles/PMC9865253/ /pubmed/36677111 http://dx.doi.org/10.3390/mi14010051 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Slotvitsky, Mikhail Berezhnoy, Andrey Scherbina, Serafima Rimskaya, Beatrisa Tsvelaya, Valerya Balashov, Victor Efimov, Anton E. Agapov, Igor Agladze, Konstantin Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
title | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
title_full | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
title_fullStr | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
title_full_unstemmed | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
title_short | Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes |
title_sort | polymer kernels as compact carriers for suspended cardiomyocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865253/ https://www.ncbi.nlm.nih.gov/pubmed/36677111 http://dx.doi.org/10.3390/mi14010051 |
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