Cargando…

Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes

Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of t...

Descripción completa

Detalles Bibliográficos
Autores principales: Slotvitsky, Mikhail, Berezhnoy, Andrey, Scherbina, Serafima, Rimskaya, Beatrisa, Tsvelaya, Valerya, Balashov, Victor, Efimov, Anton E., Agapov, Igor, Agladze, Konstantin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865253/
https://www.ncbi.nlm.nih.gov/pubmed/36677111
http://dx.doi.org/10.3390/mi14010051
_version_ 1784875791095431168
author Slotvitsky, Mikhail
Berezhnoy, Andrey
Scherbina, Serafima
Rimskaya, Beatrisa
Tsvelaya, Valerya
Balashov, Victor
Efimov, Anton E.
Agapov, Igor
Agladze, Konstantin
author_facet Slotvitsky, Mikhail
Berezhnoy, Andrey
Scherbina, Serafima
Rimskaya, Beatrisa
Tsvelaya, Valerya
Balashov, Victor
Efimov, Anton E.
Agapov, Igor
Agladze, Konstantin
author_sort Slotvitsky, Mikhail
collection PubMed
description Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of the cardiomyocytes has substantial challenges related to cell survival and electrophysiological coupling with recipient tissue. Current methods of manipulating cell suspensions do not allow one to control the processes of adhesion of injected cells to the tissue and electrophysiological coupling with surrounding cells. In this article, we documented the possibility of influencing these processes using polymer kernels: biocompatible fiber fragments of subcellular size that can be adsorbed to a cell, thereby creating the minimum necessary adhesion foci to shape the cell and provide support for the organization of the cytoskeleton and the contractile apparatus prior to adhesion to the recipient tissue. Using optical excitation markers, the restoration of the excitability of cardiomyocytes in suspension upon adsorption of polymer kernels was shown. It increased the likelihood of the formation of a stable electrophysiological coupling in vitro. The obtained results may be considered as a proof of concept that the stochastic engraftment process of injected suspension cells can be controlled by smart biomaterials.
format Online
Article
Text
id pubmed-9865253
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98652532023-01-22 Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes Slotvitsky, Mikhail Berezhnoy, Andrey Scherbina, Serafima Rimskaya, Beatrisa Tsvelaya, Valerya Balashov, Victor Efimov, Anton E. Agapov, Igor Agladze, Konstantin Micromachines (Basel) Article Induced pluripotent stem cells (iPSCs) constitute a potential source of patient-specific human cardiomyocytes for a cardiac cell replacement therapy via intramyocardial injections, providing a major benefit over other cell sources in terms of immune rejection. However, intramyocardial injection of the cardiomyocytes has substantial challenges related to cell survival and electrophysiological coupling with recipient tissue. Current methods of manipulating cell suspensions do not allow one to control the processes of adhesion of injected cells to the tissue and electrophysiological coupling with surrounding cells. In this article, we documented the possibility of influencing these processes using polymer kernels: biocompatible fiber fragments of subcellular size that can be adsorbed to a cell, thereby creating the minimum necessary adhesion foci to shape the cell and provide support for the organization of the cytoskeleton and the contractile apparatus prior to adhesion to the recipient tissue. Using optical excitation markers, the restoration of the excitability of cardiomyocytes in suspension upon adsorption of polymer kernels was shown. It increased the likelihood of the formation of a stable electrophysiological coupling in vitro. The obtained results may be considered as a proof of concept that the stochastic engraftment process of injected suspension cells can be controlled by smart biomaterials. MDPI 2022-12-25 /pmc/articles/PMC9865253/ /pubmed/36677111 http://dx.doi.org/10.3390/mi14010051 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Slotvitsky, Mikhail
Berezhnoy, Andrey
Scherbina, Serafima
Rimskaya, Beatrisa
Tsvelaya, Valerya
Balashov, Victor
Efimov, Anton E.
Agapov, Igor
Agladze, Konstantin
Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes
title Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes
title_full Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes
title_fullStr Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes
title_full_unstemmed Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes
title_short Polymer Kernels as Compact Carriers for Suspended Cardiomyocytes
title_sort polymer kernels as compact carriers for suspended cardiomyocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865253/
https://www.ncbi.nlm.nih.gov/pubmed/36677111
http://dx.doi.org/10.3390/mi14010051
work_keys_str_mv AT slotvitskymikhail polymerkernelsascompactcarriersforsuspendedcardiomyocytes
AT berezhnoyandrey polymerkernelsascompactcarriersforsuspendedcardiomyocytes
AT scherbinaserafima polymerkernelsascompactcarriersforsuspendedcardiomyocytes
AT rimskayabeatrisa polymerkernelsascompactcarriersforsuspendedcardiomyocytes
AT tsvelayavalerya polymerkernelsascompactcarriersforsuspendedcardiomyocytes
AT balashovvictor polymerkernelsascompactcarriersforsuspendedcardiomyocytes
AT efimovantone polymerkernelsascompactcarriersforsuspendedcardiomyocytes
AT agapovigor polymerkernelsascompactcarriersforsuspendedcardiomyocytes
AT agladzekonstantin polymerkernelsascompactcarriersforsuspendedcardiomyocytes