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Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody

Nanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based na...

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Autores principales: Lin, Yu-Wei, Su, Hung-Cheng, Raj, Emmanuel Naveen, Liu, Kuang-Kai, Chang, Chien-Jen, Hsu, Tzu-Chia, Cheng, Po-Yun, Wang, Rou-Hsin, Lai, Yen-Her, Chen, Chien-Hung, Lin, Yen-Cheng, Chao, Jui-I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865332/
https://www.ncbi.nlm.nih.gov/pubmed/36678740
http://dx.doi.org/10.3390/pharmaceutics15010111
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author Lin, Yu-Wei
Su, Hung-Cheng
Raj, Emmanuel Naveen
Liu, Kuang-Kai
Chang, Chien-Jen
Hsu, Tzu-Chia
Cheng, Po-Yun
Wang, Rou-Hsin
Lai, Yen-Her
Chen, Chien-Hung
Lin, Yen-Cheng
Chao, Jui-I
author_facet Lin, Yu-Wei
Su, Hung-Cheng
Raj, Emmanuel Naveen
Liu, Kuang-Kai
Chang, Chien-Jen
Hsu, Tzu-Chia
Cheng, Po-Yun
Wang, Rou-Hsin
Lai, Yen-Her
Chen, Chien-Hung
Lin, Yen-Cheng
Chao, Jui-I
author_sort Lin, Yu-Wei
collection PubMed
description Nanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based nanoprobe, nanodiamond (ND), which can be applied for targeting EGFR and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo. The optimal fluorescent intensities of ND particles were observed in the human lung cancer cells and nude mice under in vivo imaging system. The fluorescence signal of ND particles can be real-time detected in the xenografted human lung tumor formation of nude mice. Moreover, the ND-conjugated specific EGFR antibody cetuximab (Cet) can track the location and distribution of EGFR proteins of lung cancer cells in vitro and in vivo. ND-Cet treatment increased cellular uptake ability of nanocomposites in the EGFR-expressed cells but not in the EGFR-negative lung cancer cells. Interestingly, single ND-Cet complex can be directly observed on the protein G bead by immunoprecipitation and confocal microscopy. Besides, the EGFR proteins were transported to lysosomes for degradation. Together, this study demonstrates that ND-conjugated Cet can apply for targeting EGFR and monitoring tumorigenesis during lung cancer progression and therapy.
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spelling pubmed-98653322023-01-22 Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody Lin, Yu-Wei Su, Hung-Cheng Raj, Emmanuel Naveen Liu, Kuang-Kai Chang, Chien-Jen Hsu, Tzu-Chia Cheng, Po-Yun Wang, Rou-Hsin Lai, Yen-Her Chen, Chien-Hung Lin, Yen-Cheng Chao, Jui-I Pharmaceutics Article Nanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based nanoprobe, nanodiamond (ND), which can be applied for targeting EGFR and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo. The optimal fluorescent intensities of ND particles were observed in the human lung cancer cells and nude mice under in vivo imaging system. The fluorescence signal of ND particles can be real-time detected in the xenografted human lung tumor formation of nude mice. Moreover, the ND-conjugated specific EGFR antibody cetuximab (Cet) can track the location and distribution of EGFR proteins of lung cancer cells in vitro and in vivo. ND-Cet treatment increased cellular uptake ability of nanocomposites in the EGFR-expressed cells but not in the EGFR-negative lung cancer cells. Interestingly, single ND-Cet complex can be directly observed on the protein G bead by immunoprecipitation and confocal microscopy. Besides, the EGFR proteins were transported to lysosomes for degradation. Together, this study demonstrates that ND-conjugated Cet can apply for targeting EGFR and monitoring tumorigenesis during lung cancer progression and therapy. MDPI 2022-12-28 /pmc/articles/PMC9865332/ /pubmed/36678740 http://dx.doi.org/10.3390/pharmaceutics15010111 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Yu-Wei
Su, Hung-Cheng
Raj, Emmanuel Naveen
Liu, Kuang-Kai
Chang, Chien-Jen
Hsu, Tzu-Chia
Cheng, Po-Yun
Wang, Rou-Hsin
Lai, Yen-Her
Chen, Chien-Hung
Lin, Yen-Cheng
Chao, Jui-I
Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody
title Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody
title_full Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody
title_fullStr Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody
title_full_unstemmed Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody
title_short Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody
title_sort targeting egfr and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo using nanodiamond-conjugated specific egfr antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865332/
https://www.ncbi.nlm.nih.gov/pubmed/36678740
http://dx.doi.org/10.3390/pharmaceutics15010111
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