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The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR Spectroscopy

The water-soluble blue–green pigment marennine, produced and partly excreted by the diatom Haslea ostrearia, and known for a long time for its role in the greening of oysters, was isolated from the culture medium, purified, and analyzed by Nuclear Magnetic Resonance (NMR) in order to gain insight in...

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Detalles Bibliográficos
Autores principales: Zebiri, Ilhem, Jacquette, Boris, Francezon, Nellie, Herbaut, Mickaël, Latigui, Amina, Bricaud, Sullivan, Tremblay, Réjean, Pasetto, Pamela, Mouget, Jean-Luc, Dittmer, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865362/
https://www.ncbi.nlm.nih.gov/pubmed/36662215
http://dx.doi.org/10.3390/md21010042
Descripción
Sumario:The water-soluble blue–green pigment marennine, produced and partly excreted by the diatom Haslea ostrearia, and known for a long time for its role in the greening of oysters, was isolated from the culture medium, purified, and analyzed by Nuclear Magnetic Resonance (NMR) in order to gain insight into its chemical structure. The spectra show mainly carbohydrates of a complex composition, apparently highly branched, and with a mass in the order of 10 kDa. There are, in addition, some signals of aliphatic and, much weaker, aromatic groups that present aglycons. The latter might be responsible for the color. These carbohydrates are always associated with the blue–green color and cannot be separated from it by most treatments; they are interpreted as constituting the frame of the pigment. NMR after hydrolysis identifies the most abundant monosaccharides in marennine as galactose, xylose, mannose, rhamnose, and fucose.