Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples

Exposure to aerosols containing Legionella from artificially made water systems has been established as a primary cause of Legionnaires’ disease. In this study, we investigated an outbreak of L. pneumophila serogroup 1 sequence type 138 which occurred at a bath facility in 2022. The whole-genome seq...

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Autores principales: Nakanishi, Noriko, Komatsu, Shoko, Tanaka, Shinobu, Mukai, Kengo, Nomoto, Ryohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865471/
https://www.ncbi.nlm.nih.gov/pubmed/36677320
http://dx.doi.org/10.3390/microorganisms11010028
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author Nakanishi, Noriko
Komatsu, Shoko
Tanaka, Shinobu
Mukai, Kengo
Nomoto, Ryohei
author_facet Nakanishi, Noriko
Komatsu, Shoko
Tanaka, Shinobu
Mukai, Kengo
Nomoto, Ryohei
author_sort Nakanishi, Noriko
collection PubMed
description Exposure to aerosols containing Legionella from artificially made water systems has been established as a primary cause of Legionnaires’ disease. In this study, we investigated an outbreak of L. pneumophila serogroup 1 sequence type 138 which occurred at a bath facility in 2022. The whole-genome sequencing of isolates revealed that the colonization of L. pneumophila at the bath facility had occurred before 2013, and the patients had been exposed to multiple genetic lineages of the strain. Our study demonstrates the importance of performing a careful comparative genetic analysis of clinical and environmental isolates from LD outbreaks in order to effectively investigate and prevent future LD outbreaks.
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spelling pubmed-98654712023-01-22 Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples Nakanishi, Noriko Komatsu, Shoko Tanaka, Shinobu Mukai, Kengo Nomoto, Ryohei Microorganisms Brief Report Exposure to aerosols containing Legionella from artificially made water systems has been established as a primary cause of Legionnaires’ disease. In this study, we investigated an outbreak of L. pneumophila serogroup 1 sequence type 138 which occurred at a bath facility in 2022. The whole-genome sequencing of isolates revealed that the colonization of L. pneumophila at the bath facility had occurred before 2013, and the patients had been exposed to multiple genetic lineages of the strain. Our study demonstrates the importance of performing a careful comparative genetic analysis of clinical and environmental isolates from LD outbreaks in order to effectively investigate and prevent future LD outbreaks. MDPI 2022-12-22 /pmc/articles/PMC9865471/ /pubmed/36677320 http://dx.doi.org/10.3390/microorganisms11010028 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Nakanishi, Noriko
Komatsu, Shoko
Tanaka, Shinobu
Mukai, Kengo
Nomoto, Ryohei
Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples
title Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples
title_full Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples
title_fullStr Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples
title_full_unstemmed Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples
title_short Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples
title_sort investigation of a legionella pneumophila outbreak at a bath facility in japan using whole-genome sequencing of isolates from clinical and environmental samples
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865471/
https://www.ncbi.nlm.nih.gov/pubmed/36677320
http://dx.doi.org/10.3390/microorganisms11010028
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